Journal of Engineering Geology

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Komrud village of located on the slope is particularly susceptible to landslides. The slope stability analysis is of special importance because of landslides or slope failures can cause major damages life and financial. In studies to determine the factors and parameters affecting the slope is unstable. According to studies, one of the most important factor affecting landslides in this area is the force exerted by the acceleration of the earthquake. With this approach, the maximum possible acceleration of earthquakes in a 50-year period is estimated at the site. On the other hand, based on studies of the geological is determined profile of the slope geometry, physical and resistance parameters to the landslide susceptible. Then using the modeling software in FLAC 2D 5.00 elasto-plastic structural models, with failure criteria Mohr – Coulomb, stress-strain behavior of the soil has been examined to pseudo-dynamic method. A base position at the top of the slope is considered and displacement it horizontally to reach the maximum possible acceleration is calculated and recorded. The results of this study indicate that Horizontal displacement followed the landslide, can be occur below the maximum acceleration estimates.

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With regard to the increase of computing power in the past decades, finite element methods have been used to obtain the graphs of rotational moment curves which reflect non-linear effect in connections response. Several common semi-rigid connections are modeled and their behavioral properties are briefly reviewed, then the details related to a new semi-fixed connection have been provided. The behavioral properties like hardness, ultimate capacity and ductility are investigated and compared to other simulated connections. To perform non-linear analyses of connection, finite element software ABAQUS is used. In this simulation, it has been tried to have inter-component interactions according to reality as much as possible. Bolted connections are modeled exactly and the interaction among the bolt surface and hole is modeled as a hard friction with friction coefficient 0.3 with the ability of separating after loading. Also, fillet welds are modeled as a prism with triangular section. Where a groove weld is applied, since the strength in this type of welding is like base metal, two connection parts are stuck together. To mesh the element, C3D8R element is used. The proposed connection n1 has the most rigidity values among semi-rigid connections. Reducing the number of connection bolts has more reducing impact on connection rigidity value, so that with the half thickness of upper and lower sheets, rigidity rate is reduced only 9%, but with the half number of bolts, rigidity rate is reduced about 64%. Also the connection n3 have lowest rigidity rate and its rigidity amount is in the class of bolted connection in seat angle to web angle.

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Introduction
Microbial induced calcite precipitation (MICP) is one of the environment-friendly soil improvement methods that uses urease activity of the microorganisms to bound soil grains.
This method is based on three following steps:
1. Urea hydrolysis by urease activity of microorganisms and formation of ammonium and carbonate ions:
(2)
2. The reaction between carbonate and calcium ions and formation of calcium carbonate:
(2)
3. Bonding the soil particles by calcium carbonate.
One of the main challenges in use of MICP for soil improvement is the selection of proper injection method. An efficient injection method should lead to the construction of a homogeneous specimen beside of less used materials. In this study, a new method based on the theory of convection of liquids, for injection of bacteria and cementation solution is introduced.
Specimens are made according to the new injection method and their strength and homogeneities are tested. The obtained results are compared with the specimens which are made based on common injection method. Eventually, the success of the proposed injection method is investigated.
 
Material and methods
Gram-positive microorganism Sporosarcina Pasteurii No. 1645 (DSM 33) is provided from Persian type culture collection (PTCC). To make sand columns, Poly Vinyl Chloride (PVC) tubes were used with an internal diameter of 5cm and length of 12cm. Molds were placed vertically and a scouring pad and approximately 1 cm gravel as a filter are placed at the bottom of the column. Then the column packed with pure silica (Table 1). Finally, a scouring pad and approximately 1 cm gravel as a filter are placed at top of the column and mold were closed with a threaded Polypropylene layer on top and bottom with a hole for injection of bacteria and cementation solutions.
Table 1. Sand properties used in this study

Soil Type	Gs	γd	e	D10	D30	D60
Sw	2.6	1.84	41%	0.11 mm	0.43mm	0.85mm

In this study, a new multi-step method of injecting bacterial and cementation solutions is introduced. Injection of solutions is done after washing the sand column with distilled water. At the first step, 0.25 times of the void volume of soil, the bacterial solution is injected into the sand column. The bacteria allowed resting in the sand for 2 hours before the cementation solution was injected. After 2 hours, cementation solution is injected into the sand column by the amount of 0.25 times of pore volume of soil. The cementation solution consisted of 1.5 M urea and 3 M Calcium chloride. Again after 2 hours delay, bacterial solution and cementation solution are injected into sand column both by the amount of 0.25 times of pore volume of soil, same as aforementioned steps. In order to provide a comparison between the proposed injection methods of this study with conventional injection method, specimens are also made by the conventional method. In these specimens, bacterial solution and cementation solution are injected into the soil both by the amount of 1.5 times of pore volume of soil.
Results and discussion
To evaluate the homogeneity of the biologically improved sand specimens, the specimen is divided into 6 equal parts and the amount of calcium carbonate in each part is measured. It is found that calcium carbonate crystals are formed more homogenous in parts of specimens which are improved by new injection method (Figure 1). While specimens improved with conventional injection method are not homogeneous. The new injection method used in this study is based on the theory of convection in cementation and bacterial solution. Since the specific gravity of used cementation solution (3M urea and 1.5M calcium chloride solution) is 1.120 gr/cm3 and the specific gravity of ammonium chloride (which is the result of reaction between ammonium and chloride ions) is 1.031 gr/cm3, therefore a convection flow occurs in cementation solution after urease reaction (reaction 1) because of difference in specific gravity of two mentioned solutions. This convection flow causes a sustainable contact between cementation and bacterial solution in entire height of specimen.

Figure 1. Amount of calcium carbonate deposition along improved specimens by new and conventional injection method
To examine the efficiency of newly suggested injection method in this study, uniaxial compressive strength test (UCS) is performed on biologically improved sand specimens. Figure 2 shows stress-strain curves of specimens. The peak strength of specimens with conventional injection method is about 0.6 MPa. While the peak strength of biologically improved specimens prepared by new injection method is about 1.6 MPa. The reason for this difference in the obtained results is that when the volume of bacterial solution is more than the pore volume of soil, a part of bacteria solution in the first step of injection is removed. Then with an injection of cementation solution, more amounts of bacteria removes from the specimen before efficient placement of bacteria between soil particles. However, in new injection method the total volume of injection solutions (bacterial and cementation solutions) are equal to the pore volume of soil and this prevents the removal of bacteria from a porous medium.

Figure 2. Uniaxial stress-uniaxial strain curves of biologically improved specimens
Conclusion
In this study, the feasibility of using a new injection method for biological soil improvement is investigated based on the theory of convection with the aim to decrease the volume of bacteria and cementation solution. In this method, the final volume of bacterial and cementation solutions are reached the soil void volume in 4 consecutive injection steps. Specimens are made to investigate the efficiency of the proposed injection method. Also, specimens are made base on conventional injection method to provide the comparing possibility. Studying the precipitated calcium carbonate along the specimens show more homogeneity in ones prepared by proposed injection method in comparison to the specimens made by the conventional method. The obtained results of UCS tests are also showed that specimens made by new injection method have the more uniaxial strength (1.6 MPa) while the conventional method specimens are presented the strength of 0.6 MPa. Eventually, the proposed injection method of this paper implies less amount of bacterial and cementation solutions in a proper and efficient manner to bond the soil particles which leads to specimens with more strength, stiffness and homogeneity../files/site1/files/131/2Extended_Abstract.pdf
 

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Introduction
Improving the geotechnical characteristics of soils including superficial or deep soils has always been a challenge to geotechnical engineers. Therefore, various physical and chemical methods are used to improve different types of soils. In general, any physical, chemical, biological or combination of methods are used to change the characteristics of natural soil mass in order to achieve engineering goals which is defined in the "soil stabilization." Among different types of additives for soil stabilization, the use of pozzolans has been investigated by researchers because of their chemical compatibility with the environment and the cementation products due to chemical reactions. Todays, a lot of researches has been done on the use of natural or artificial zeolites as pozzolanic materials for the production of cement mixtures. This material, as a pozzolan, increases the speed of the pozzolanic reactions and reduces the density of cement products. However, many studies have been done to investigate the effect of zeolite and sepiolite on the resistance of cement products such as concrete, but so far, the use of these additives has been less considered for soil improvement. On the other hand, because of the compatibility of zeolite and sepiolite with the environment and their unique physiochemical properties, it is necessary to pay attention to these additives in order to improve the soil. Therefore, in this research, the effect of zeolite and sepillot additives with different percentages at different treatment times have been investigated to determine the elasticity modulus and hydraulic conductivity with focus on soil microstructure behavior.
Materials and methods
1. The properties of the soils
In this research, two types of soil including clayey sand (with 20% clay) and sandy clay (with 51% of clay) were used. The studied soils were a mixture of clay and sand of Firoozkouh (a typical type of sand located in north of Iran). Some physiochemical properties of zeolite and sepiolite are presented in Table 1.
Table 1. Physiochemical properties of zeolite and sepiolite used in this study

L.O.I.	Na2O	K2O	MgO	CaO	Fe2O3	Al2O3	SiO2		Chemical component
25.11	0.02	0.01	15.73	0.01	o.61	0.3	55.3		Sepiolite (%)S
11.94	0.13	-	0.87	2.45	1.26	13.54	69.74		Zeolite (%)

2. Experiments
The uniaxial compressive strength tests were performed at 0.1 mm/min according to ASTM D2166 standard. The stabilized soil samples were compacted at percentages of 0, 5, 10, 15, 20 and 25 in cylindrical molds (38mm × 76mm) in five layers to achieve the desired density. In order to investigate the effect of curing time, the samples were placed inside sealed containers and underwent the test at instantaneous, 7, 14, and 28 days and at the desired additive percentages. To investigate the effect of additives on the soil hydraulic conductivity, clayey sand soil with additives 5, 10, 15, 20, and 25% was prepared using dry mixing method. Then, the prepared mixture was poured from a specific height into the permeability mold with a height of 8.65 cm and diameter of 5 cm. In this way, the specific dry unit weight of all samples was obtained as 1.47 g/cm³, close to the minimum specific dry unit weight. In this research, concerning the considerable effect of fine-grained soils on hydraulic conductivity, falling head test was used to determine the permeability coefficient.
In order to the morphology of the clayey sand soil without additives and stabilized with additives 15% was examined through SEM test.
Discussion and results
1. Modulus of elasticity
In this study, after uniaxial tests in different percentages and ages, the stress-strain graphs were plotted and then the elasticity modulus was calculated. The results showed that, with increasing zeolite content, the modulus of elasticity has been increased and, with increasing curing time, except for a slight decrease, after 7 days, the modulus of elasticity increased. During the initial treatment (7 days), the hardness of the sandy clay soil decreased and then increased with increasing time. In general, hardness in both soils in the high percentages of zeolite is significantly is increased.
Also, the effect of sepiolite on the modulus of elasticity has been studied. The results indicate that with the increase in the percentage of additive and lengthening the curing time, the modulus of elasticity is increased. This increase in the stabilization of both sandy clay and clayey sand soil is almost the same. In addition, in the case of sepiolite modification, the elasticity of sandy clay and clayey sand is approximately equal to 5 times in comparison to the initial value of unstabilized soil. However, in zeolite, the modulus of elasticity in clayey sand soils is almost 2 times, and sandy clay is nearly 5 times higher.
2. Permeability
To investigate the effect of additives on the soil hydraulic conductivity, clayey sand soil with additives 5, 10, 15, 20, and 25% was prepared using dry mixing method. The samples were saturated in a short period and permeability test was carried out immediately. Permeability coefficient changes were mostly influenced by physical factors. Therefore, due to the fineness of both types of additives, the hydraulic conductivity decreases with increasing additive content. The amount of reduced hydraulic conductivity in sepiolite stabilization is greater than zeolite due to the structure of the sepiolite (fiber-shaped) compared to zeolite.
 
 
3. SEM imaging
In this study, attempts were made to examine the reasons behind the obtained results more carefully through SEM imaging.

c                                     b                              a
Figure 1. SEM image of non-stabilized clayey sand soil (a) soil stabilized with zeolite 15% (b) soil stabilized with sepiolite 15% (c) during the curing time of 28 days at magnifications 10000X
Figure 1a displays the SEM image of non-stabilized clayey sand soil. As can be seen in the figure, the soil structure is clear as layered and clay scales can be seen as laminated. Figure 1b demonstrates the SEM images of clayey sand soil stabilized with zeolite 15% during the curing time of 28 days. The sample has lost its layered structure in response to stabilization with zeolite during the curing time and changed into an integrated structure. This can be due to incidence of chemical reactions such as ion exchange and pozzolanic reactions in response to adding zeolite. Figure 1c demonstrates the SEM images of clayey sand soil stabilized with sepiolite 15% during the curing time of 28 days. As shown in the figure, the sepiolite has a fibrous-shaped structure that is longitudinally twisted. Also, with  curing time increase, complex structures have emerged that could be due to the occurrence of chemical reactions.
Conclusion
This study examined the effect of zeolite and sepiolite additives on strength parameter of clayey soils. Accordingly, uniaxial compressive strength test was performed on clayey sand and sandy clay soil at percentages of 0, 5, 10, 15, 20 and 25% of zeolite and sepiolite with instantaneous curing times of 7, 14 and 28 days. Further, permeability test was conducted at different percentages on stabilized clayey sand soil. Also, to investigate the effect of these materials on soil microstructure, SEM imaging was performed at 28 days. The results show that both additives increase the elastic modulus of clayey sand and sandy clay soils. Also, the results indicate a steady increase in the stiffness of the cured soil with sepiolite during processing time. However, reducing soil hardness can be seen in stabilizing with zeolite at lower rates and lower percentages. In permeability test, hydraulic conductivity decreases with increasing additive content. The rate of permeability reduction in sepiolite is higher than zeolite. SEM images show that chemical reactions create an integrated structure that ultimately increases uniaxial compressive strength and modulus of elasticity. Also, SEM imaging depicts physical changes along chemical reaction in soil stabilized with sepiolite. Ultimately, increasing soil strength resulting from additive alongside environmentally friendliness is recommended in superficial and deep improvement of soil../files/site1/files/144/Rajabi.pdf
 

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