Volume 10, Issue 4 (2-2011)
2011, 10(4): 989-998 |
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Mohamadian J. Isolation and purification of purple membrane from Halobacterium salinarum and study the spectrophotometry absorbance property, change pH and absorbance of microwave radiation. Journal title 2011; 10 (4) :989-998
URL: http://jsci.khu.ac.ir/article-1-1404-en.html
URL: http://jsci.khu.ac.ir/article-1-1404-en.html
1- Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
Abstract: (7990 Views)
Halophilic bacteria can not growth without NaCl and they have optimum growth in high salinity media. Halobacteria are chemoorganotroph and the most species are aerobic. Bacteriorhodopsin is a light driven proton pump that found only in halobacteria. In this study H. salinarum that isolated in previous research from saline environment in Iran were used for isolation of purple membrane. The method of Yucel et al (1995) was applied for isolation of purple membrane of H. salinarum. The stages of this method include dialysis and centrifuge at high speed. Spectrophotometery analysis at 560 nm and absorbance ratio 280/560 nm were used for conform the purity of purple membrane. The activity of purple membrane was assayed as change of pH in salt solution contains MgCl2 and KCl. By using of microwave radiation apparatus the ability of microwave absorbance were measured. The result of isolation of purple membrane indicated that the 280/560 absorbance ratio in this spectrum was 3/3 that confirm the purity of purple membrane. Extracted purple membrane from 4 liter of culture media was 35 milligram. This extracted PM could decrease of pH (0.15 units per 6 hours) and absorb 60 % of microwave radiation. In conclusion there is very diversity between halophilic archaea in Iran ecosystem and extracted PM is favorable for nanotechnology application
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