Cyanobacteria is a big group of monera kingdom, that high potential for product some natural product, such as nutritional, pharmacologic material and etc. Oscillatoria is a kind of filamentous cyanobacteria. This microorganism observed in Tyab – bay in all of seasons. Seawater medium (SWM) is the best media for isolation of Oscillatoria from natural environment. Enriched SWM with B12 and semi-solid Z8 medium (0/7% agarose) is too suitable for purification of condense culture. Maximum coefficient (µ) was in valance medium. Purification of Oscillatoria has down at during nine mounths. Oscillatoria was exposed to five treatments of light /dark periods and six different temperatures. Results obtained revealed that among the employed 30°С treatment has had the best effect on increase maximum coefficient (µ=0.602) and also another treatment among the employed 14/10 (light / dark) hours (µ =0.506). In this treatment temperature was 25 °С.

Keywords: Cyanobacteria, Oscillatoria, optimization

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