Effects of various CuCl2 trearments (0, 100, 300, 500 µM) at rosette stage on accumulation of Cu+2, Fe+2, Mg+2, and K+, and proline concentration in root and leaf of two canola (Brassica napus L.) cultivars (PF and Hyola) were studied under greenhouse conditions. In general, in response to copper chloride treatments, significant differences were observed between physiological behaviors of these cultivars. With increasing in CuCl2 concentration, the increases in copper and proline levels and decrease in Fe+2, Mg+2, and K+ concentrations were greater in PF (especially root) than that of Hyola. Thus, it seemed that PF had more ability against heavy metal stress, compared with Hyola. Therefore, from biochemical point of view, PF could be introduced as more tolerant cultivar to heavy metal stress, in comparison to Hyola.

Keywords: copper stress, mineral cations, proline, Canola cultivars

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