Showing 5 results for Protein
Volume 8, Issue 2 (10-2009)
Abstract
In order to consideration and distinguishing of Mondell pine species (Pinus eldarica) and its two natural generated morphotypes, needles samples were collected from three years old seedlings in similar conditions and then extracted. Protein band patterns of the samples were studied and compared using electrophoresis method on polyacrylamide gel in the SDS-PAGE system. 12 morphological traits related to seedlings and mature individuals of the three pines, compared with each other. The amount of total protein also obtained using Bradford procedure and content of chlorophyll a and b measured by spectrophotometer with Harborne method. One dimensional electrophoresis of proteins showed perfectly similar isozyme patterns for each type (i.e. Mondell pine, Ball-shaped and Conical-shaped pines). Furthermore the amount of total protein and chlorophyll a and b contents of samples did not showed significant difference using ANOVA test. But 11 features of morphological measured traits were more in Mondell pine than the new generated pines. These results indicate the nonexistence of biochemical differences among these pines in spite of obvious structural and morphological differences.
Volume 9, Issue 4 (10-2010)
Abstract
The effect of various CuCl2 concentrations at rosette stage on protein level, catalase and peroxidase activities of leaf and root of two canola cultivars were studied under greenhouse conditions. The experiment was conducted as factorial in completely randomized design with 4 replications and 4 treatments (0, 100, 300, and 500µM CuCl2). In general, protein concentration and catalaze and peroxidase activities were increased with increasing CuCl2 concentration. This enhance was more in PF cultivar than Hayola, and was more in root than leaf. Thus, it seems that PF cultivar has more ability in protein biosynthesis and antioxidant enzymes activation in compare with Hyola in stress conditions. Therefore, PF can be introduced as a cultivar with more tolerance to cupper stress, in compare with Hayola cultivar.
Hajar Azarin, ,
Volume 12, Issue 3 (11-2012)
Abstract
In this study, gammaruses were collected from southern coast of Caspian Sea in Bandar Anzali. Samples were divided into three groups control, dried and frozen. These treatments were examined with several experiments such as protein, fat, sah and moisture in three time after a week, two weeks and a month in three replications. The results showed that the Analyze- Variance among the groups had significant differences in the average amount of protein, ash and moisture, but for fat no significant difference interaction between time and maintenance. In additional, by comparing of these factors by the Duncan test, in the dried samples greatest amount of protein (43/13%), fat (5/78%) and ash (23/25 %) were higher than those of in control samples, (protein: 12/36%, fat: 0/41%, ash: 5/82% and moisture: 75/67%) while control samples had higher moisture than dried samples. In all samples, control treatment had lowest fat (0/41%) and protein (12/36%), while dried samples had lowest moisture.
Farzaneh Zandi, Ramin Hossini, Neda Soltani, , ,
Volume 12, Issue 3 (11-2012)
Abstract
Oil pollutions as a consequence of increasing consumption of petroleum, have a tremendous effect on water and soil ecosystems and their microflora. In this study the diversity in physiological parameters, including growth rate, photosynthesis, chlorophyll-a and phycobiliproteins content, between cyanobacterial strains flora isolated from oil-polluted and non-polluted areas, were assessed. To this end, strains were isolated and purified. The experiments were carried out on logarithmic phase cultures of the isolates in suitable liquid media. The chlorophyll and phycobiliproteins contents were determined using a colorimetric method. Photosynthesis was measured by Oxyview apparatus. Growth rates were calculated based on dry weights. Under laboratory conditions, the photosynthesis activity and the chlorophyll content of isolates from oil-polluted sites were significantly lower than non-polluted isolates. On the other hand, the growth rate average of strains from oil-polluted sites was significantly higher than non-polluted strains. Also despite the higher phycobiliproteins content in non-polluted isolates, there was no significant difference in any of these pigments between the two groups. Altogether the results showed that in response to petroleum stress, the cyanobacteria restore the decrease in their chlorophyll content and photosynthesis activity by increasing their biomass. In fact the response is the reflects of restructuring cyanobacterial flora from sensitive autotrophic species to oil-pollution resistant mixotrophic species in the polluted ecosystems that in addition to photosynthesis are able to use crude oil as an energy source for their growth requirements, hence overcome the energy loss due to reduction of photosynthesis and even increase growth rate than non-oil-pollution isolates.
Volume 18, Issue 56 (9-2006)
Abstract
The mechanism of soybean(Glycine max L.cv.Gorgan 3) response to drought stress was evaluated. The plants were grown in pots under 23 ± 2°C temperature and 14000 lux light intensity and 14hr photoperiod. When first leaf appeard, three irrigation treatments occurred that consisted as one every day(control), once in three(mild stress) and in five days(sever stress).During 15 days the plants were irrigated an amount of 150 ml. The effect of irrigation treatment on the soluble sugar, total protein, proline, phenolic compound, chlorophyll content and nitrate reductase activity was studied.The results showed that in the root soluble sugar and proline content in mild and sever stress significantly increased while in the stem and the leaf these compound considarably increased under sever stress the total protein ,chlorophyll a,b and nitrate reductase activity in leaf especially decreased in sever stress. Also the decrease phenolic compound in leaf under this condition was significant
