Showing 2 results for Follicle
Maryam Kakaei, Zahra Nazari, Latifeh Karimzadeh, M Nabiuni,
Volume 13, Issue 1 (5-2013)
Abstract
The in-vitro culture of immature ovarian follicles is used to examine the factors that regulate follicle development and may ultimately provide options for reproductive infertility. Normal follicular development of an oocyte that is capable of fertilization and embryonic development depends upon a complex sequence of cellular interactions within the follicle . Honey Bee Venom (HBV) contains some biological active components which has been demonstrated it,s promoting effect on ovulation up to 70 % in rats . The aim of this research is study of the effect of HBV on in-vitro maturation of different type of preantral follicles. In this research, unmature follicles were taken from 14 day-old mice and cultured in &alpha-minimal essential medium (&alpha-MEM) and divided to small, medium and large preantral follicles and treated with 1µg/ml BV. Then diameter of follicle was measured until 4th days. Finally, morphological appearance of mature oocytes was studied. Studies of follicle diameters and flowcytometry showed HBV led to an increase in follicle diameters (p<0.001). Amount of GV was 53 %(control group) and 31.4 (treatment group)that was significative (p<0.001). Developing up to meiosis I was 30% (control group) and 43.2% (treatment group) and developing up to meiosis II was 15% (control) and 22.3% (treatment group). In brief, we are confirmed that honey bee venom has led to significant changes in in-vitro maturation of preantral follicles and consequently, better performance for fertilization.
A Gharzi,
Volume 15, Issue 1 (4-2015)
Abstract
Formation of feather and hair follicles during embryonic stage is nearly similar but whether they possess a similar structure and organization in mature stage as well? The aim of the present research is to study similarities and differences of these two follicles in adult rats and pigeons. To fulfill this task, after removing follicles from the skin, the follicles processed for light and transmission electron microscopy. The results provided here demonstrated that these two follicle types are similar in having the dermal papilla, epidermal matrix and epidermal stem cells. Moreover, the production of hair and feather fibers in them is almost the same. One of differences seen between these follicle is that, unlike the hair follicle in the feather follicle a distinct compartment, called the pulp, is observed at the top of the dermal papilla. At the center of this pulp as well as the dermal papilla a blood vessel exists, a character which never seen in the hair follicle. Another difference observed is related to the thickness of the basement membrane exists at the interface between the papilla and epidermal matrix. This membrane in feather follicle is much thicker than its hair counterpart. In addition, at the basal region of the feather follicle there is a distinct population of stem-like cells which could not be distinguished in the hair follicle. Based on results provided here it seems that the feather in birds and the hair in mammals have been adapted for different biological tasks.
