Volume 18, Issue 56 (9-2006)
Abstract
Methods of plants tissues and organs culture were used for micropagation, production of plants free from contamination especially viral contaminations preparation of plants for transforming, improvements and cloning. Culture of Canola has developed recently in Iran and is used for vegetable oil and protein. Our target in this study was micropropagation, induction of calli and plantlet regeneration by tissue culture. Explants of Pioneer cultivars from Canola were cultured in MS medium with different concentrations of PGRs (plant growth tegulators) such as IBA (Indol butyric acid), BA (benzyl adenin), 2,4-D (2,4 dichlorophenoxy acetic acid. Results of repeated experiments indicated that in MS medium with 2 mg/lit BA, and 1-2 mg/lit IBA plus 1 mg/lit BA induced white calli that become organogen when diameter reached to 5-6 mm. MS medium with 2 mg/lit BA and different concentrations of 2,4-D (2-10 mg/lit) induced yellow calli that were alike embryogen calli and either longitudinal or transversal sections proved embryogenic structures in primary steps in them. In MS medium with out of 2 mg/lit IBA induced brown calli that grew slow and died.
