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Volume 9, Issue 4 (10-2010)
Abstract

The effect of various CuCl2 concentrations at rosette stage on protein level, catalase and peroxidase activities of leaf and root of two canola cultivars were studied under greenhouse conditions. The experiment was conducted as factorial in completely randomized design with 4 replications and 4 treatments (0, 100, 300, and 500µM CuCl2). In general, protein concentration and catalaze and peroxidase activities were increased with increasing CuCl2 concentration. This enhance was more in PF cultivar than Hayola, and was more in root than leaf. Thus, it seems that PF cultivar has more ability in protein biosynthesis and antioxidant enzymes activation in compare with Hyola in stress conditions. Therefore, PF can be introduced as a cultivar with more tolerance to cupper stress, in compare with Hayola cultivar.

Volume 18, Issue 45 (6-2008)
Abstract

Effects of various CuCl2 trearments (0, 100, 300, 500 µM) at rosette stage on accumulation of Cu+2, Fe+2, Mg+2, and K+, and proline concentration in root and leaf of two canola (Brassica napus L.) cultivars (PF and Hyola) were studied under greenhouse conditions. In general, in response to copper chloride treatments, significant differences were observed between physiological behaviors of these cultivars. With increasing in CuCl2 concentration, the increases in copper and proline levels and decrease in Fe+2, Mg+2, and K+ concentrations were greater in PF (especially root) than that of Hyola. Thus, it seemed that PF had more ability against heavy metal stress, compared with Hyola. Therefore, from biochemical point of view, PF could be introduced as more tolerant cultivar to heavy metal stress, in comparison to Hyola.

Volume 18, Issue 56 (9-2006)
Abstract

Methods of plants tissues and organs culture were used for micropagation, production of plants free from contamination especially viral contaminations preparation of plants for transforming, improvements and cloning. Culture of Canola has developed recently in Iran and is used for vegetable oil and protein. Our target in this study was micropropagation, induction of calli and plantlet regeneration by tissue culture. Explants of Pioneer cultivars from Canola were cultured in MS medium with different concentrations of PGRs (plant growth tegulators) such as IBA (Indol butyric acid), BA (benzyl adenin), 2,4-D (2,4 dichlorophenoxy acetic acid. Results of repeated experiments indicated that in MS medium with 2 mg/lit BA, and 1-2 mg/lit IBA plus 1 mg/lit BA induced white calli that become organogen when diameter reached to 5-6 mm. MS medium with 2 mg/lit BA and different concentrations of 2,4-D (2-10 mg/lit) induced yellow calli that were alike embryogen calli and either longitudinal or transversal sections proved embryogenic structures in primary steps in them. In MS medium with out of 2 mg/lit IBA induced brown calli that grew slow and died.

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