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Volume 18, Issue 58 (8-2004)

In this investigation, quantitative analysis of glycyrrhizic acid (GA) in roots and calluses from different populations of Glycyrrhiza glabra L. were performed by HPLC. For this purpose roots and seeds were collected from localities of Abadeh (Fars), Djupar (Kerman) and Veyssian (Lorestan). For callus production, root explants of ten days old seedlings were maintained on Murashige and Skoog medium with 2,4-D (0.5 mg.l-1), IAA (0.5 mg.l-1) and BAP (0.1 mg.l-1). After 42 days, the growing calluses were excised and used for GA analysis. High amounts of GA were found in roots but no significant differences were observed between roots from different populations contrary to calluses. Between calluses of different origin, the highest content of GA (still remaining very small compared with the roots one) was observed in callus of G.glabra L. var. glabra of Fars. The lowest level of GA was noticed in calluses of G.glabra L. var. glandulifera of Fars, which have shown the maximum of growth in vitro.

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