TY - JOUR T1 - Effects of 50 Hz alternative electromagnetic fields, using solenoid coils on Balb/C mice limb buds development in in vitro TT - بررسی آثار میدان الکترومغناطیسی بر رشد جوانه‌های اندام حرکتی فوقانی وتحتانی جنین موش نژاد Balb/C در شرایط in vitro JF - jsci JO - jsci VL - 18 IS - 50 UR - http://jsci.khu.ac.ir/article-1-1158-en.html Y1 - 2005 SP - 315 EP - 327 KW - مورفولوژیکی وهیستولوژیکی نمونه‌ها N2 - Over the past two decades, electeromagnetic fields (EMFs) have been used effectively to treat diseases, such as musculoskeletal and chondrogenic disorders. Proper application of EMFs is presumed to stimulate osteogenesis. To investigate the effects of EMFs on limb bud development in in vitro, an organ culture system, using Eagle’s minimum essential medium (Eagle’s MEM), supplemented with 15% human embryo cord serum, was used. Three test groups of amputated Balb/C mouse limb buds (n=70), included experimental (E) exposed to EMFs, sham (sh) exposed groups with no exposure to EMFs and control (C). Limb buds of two groups (E and sh) were amputated from mouse embryos, on day 11.5 of gestation and cultured for 2 days the group C was removed, on day 13.5. Morphological and histological examinations showed significant changes occured in experimental groups, comparing with sham and control groups. In both fore and hind limbs, development in proximo-distal (P-D) and anterio-posterior (A-P) axes was significant (P M3 ER -