@ARTICLE{Nabiuni, author = {KAKAEI, MARYAM and Nazari, Zahra and Karimzadeh, latifeh and Nabiuni, M and }, title = {Effect of honey bee venom on in vitro maturation of preantral follicles in NMRI mice}, volume = {13}, number = {1}, abstract ={The in-vitro culture of  immature ovarian follicles is used to examine the factors that regulate follicle development and may ultimately provide options  for  reproductive  infertility. Normal follicular development of an oocyte that is capable of fertilization and embryonic development depends upon a complex sequence of cellular interactions within the follicle . Honey Bee Venom (HBV) contains some biological active components which has been demonstrated it,s promoting effect on ovulation up to 70 % in rats . The aim of this research is study of the effect of HBV on in-vitro maturation of different type of preantral follicles. In this research, unmature follicles were taken from 14 day-old mice and cultured in &alpha-minimal essential medium (&alpha-MEM) and divided to small, medium and large preantral follicles and treated with 1µg/ml BV. Then diameter of follicle was measured until 4th days. Finally, morphological appearance of mature oocytes was studied. Studies of follicle diameters and flowcytometry showed HBV led to an increase in follicle diameters (p }, URL = {http://jsci.khu.ac.ir/article-1-1555-en.html}, eprint = {http://jsci.khu.ac.ir/article-1-1555-en.pdf}, journal = {Journal title}, doi = {}, year = {2013} }