دوره 6، شماره 4 - ( 10-1398 )
جلد 6 شماره 4 صفحات 445-435 |
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Zadeh-Hosseingholi E, Chaparzadeh N, Mahmudi Aghdam S. Isolation and characterization of plant growth-promoting bacteria from Syrian bean caper (Zygophyllum fabago) rhizosphere
. NBR 2020; 6 (4) :435-445
URL: http://nbr.khu.ac.ir/article-1-3046-fa.html
URL: http://nbr.khu.ac.ir/article-1-3046-fa.html
زاده حسینقلی الهه، چاپارزاده نادر، محمودی اقدم سمیرا. جداسازی و شناسایی باکتری های محرک رشد از ریشه سپهر گیاه اسپندک. یافته های نوین در علوم زیستی. 1398; 6 (4) :435-445
دانشگاه شهید مدنی آذربایجان ، nchapar@azaruniv.ac.ir
چکیده: (3342 مشاهده)
برخی از ریزوباکتری ها اثرات مفید بر رشد گیاهان دارند. اسپندک (Zygophyllum fabago) یک علف هرز با ارزش دارویی است. در تحقیق حاضر باکتری های ریشه سپهر این گیاه جداسازی و شناسایی شدند. خصوصیات مرتبط با تحریک رشد گیاه مانند انحلال فسفات و روی، تولید ایندول استیک اسید و فعالیت ضد قارچی جدایه ها مورد برر سی قرار گرفت. سپس جدایه ها جداگانه به گیاه تلقیح شده و پس از حصول اطمینان از استقرار آن ها در ریشه، کارایی آن ها در افزایش رشد گیاه در شرایط گلخانه ای سنجیده شد. نتایج شناسایی بیوشیمیایی و مولکولی نشان داد که 5 جدایه حاصل متعلق به جنس های Bacillus، Pseudomonas، Pantoea و Brevibacterium بودند. همه 5 جدایه درجاتی از توانایی در تحریک رشد گیاه را نشان دادند. از میان جدایه ها، فقط جدایه متعلق به جنس Bacillus توانست وزن خشک گیاهان را به طور معنی داری افزایش دهد. میزان انحلال فسفات این جدایه برابر با 440 میکروگرم در میلی لیتر و میزان تولید اسید آن در محیط کشت بیش از سایر جدایه ها بود. جدایه فوق با داشتن قدرت انحلال عنصر روی، ایندول استیک اسید را به مقدار 89/3 میلیگرم در میلی لیتر تولید کرد. با این وجود این جدایه فعالیت ضد قارچی بر علیه دو قارچ بیماری زای گیاهی Aspergillus niger و Botrytis cinerea را نشان نداد.
نوع مطالعه: مقاله پژوهشی |
موضوع مقاله:
میکروبیولوژی
دریافت: 1396/10/12 | ویرایش نهایی: 1398/12/5 | پذیرش: 1398/8/13 | انتشار: 1398/10/18 | انتشار الکترونیک: 1398/10/18
دریافت: 1396/10/12 | ویرایش نهایی: 1398/12/5 | پذیرش: 1398/8/13 | انتشار: 1398/10/18 | انتشار الکترونیک: 1398/10/18
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