Investigation the anticancer effect of caffeic acid in carbon nanotube with and without chitosan coating on human cervical cancer HELA cell line

Azdeh Mohamadgholi

The aim of this study was to investigate the effect of cofactor-bearing chitosan-coated and non-chitosan carbon nanotubes on induction of apoptosis of cervical cancer HELAcell line by measuring the expression of Bax and Bcl-2 genes as well as cell growth and proliferation. MTT test was used for cell proliferation assay. Expression changes of Bax and Bcl-2 genes were analyzed by Real Time PCR. The MTT results showed that the carbon nanotube coated with caffeic acid carrier chitosan and without caffeic acid carrier and crude carbon nanotube and caffeic acid induce apoptosis and cell toxicity. The results showed that caffeic acid with a chitosan-coated carbon nanotube carrier increased Bax expression and decreased Bcl-2 gene expression, so that the expression ratio of Bcl-2 / Bax compared to free caffeic acid to significantly increase. The results show that the death of cancer cells treated with caffeic acid with chitosan-coated carbon nanotube carries an increase in Bcl-2 / Bax expression ratio and thus induces apoptosis in HELA cancer cells. . Also, the use of caffeic acid as a drug and carbon nanotube-coated carbon nanotube carriers of caffeic acid can be considered as a promising strategy in the treatment of cervical cancer.

The effect of sowing date and seed pretreatment with growth regulators on morphophysiological traits and yield of wheat (Triticum aestivum L.) under late spring cold stress conditions

Esmaeil Gholinezhad

Objective: This research was conducted to investigate the effects of sowing date and different seed priming treatments on morpho-physiological traits and grain yield of Simin wheat cultivar under late spring cold stress conditions in West Azerbaijan province.
Methods: The experiment was carried out as a factorial arrangement in a randomized complete block design with three replications during the 2024-2025 growing season. The first factor included two sowing dates (November 21 and December 22) and the second factor consisted of seed priming treatments.
Results: The results indicated that the first sowing date showed significant superiority in all traits and led to a 38% increase in grain yield compared to the second sowing date. Among priming treatments, melatonin was identified as the most effective treatment with 37% and 42% increase in grain yield in the first and second sowing dates, respectively. In contrast, salicylic acid treatment in the second sowing date caused 92% reduction in grain yield ratio and 75% reduction in biomass production efficiency.
Conclusion: Optimal sowing date along with the application of priming treatments such as melatonin and gibberellic acid can be recommended as an effective strategy for improving grain yield and late spring cold stress tolerance in wheat.

Nova Biologica Reperta

Investigation the anticancer effect of caffeic acid in carbon nanotube with and without chitosan coating on human cervical cancer HELA cell line

The effect of sowing date and seed pretreatment with growth regulators on morphophysiological traits and yield of wheat (Triticum aestivum L.) under late spring cold stress conditions