Showing 354 results for Type of Study: Original Article
Fereshteh Mohamadhasani Javar, Mehdi Rahimi,
Volume 10, Issue 2 (9-2023)
Abstract
Heavy metal contamination (HMs) of water and soil is the most serious problem caused by industrial and mining processes and other human activities. Mycoremediation is a biotechnological method that employs fungi to remove toxic contaminants from the environment in an efficient and cost-effective manner. Pleurotus species are considered to be the most popular and widely cultivated species worldwide, and this may be due to their low production cost and high yield. It has been indicated that Pleurotus species may improve plant growth in metal-contaminated soils through enhancing nutrition uptake or by alleviating toxicity of the metals. In this experiment, the fungus was grown in vitro in liquid and solid media for 3 weeks on five different concentrations (0, 15, 30, 45, 60 ppm) of five heavy metals (Cd, Zn, Ni, Pb, Mn) as sulphate and the effect of these metal on radial growth, biomass production and metal content of fungal biomass were determined. Based on the results, this fungus showed a great variety of tolerance against the metals, as that growth parameters were increased in the case of two metals contamination (Zn and Mn) and inhibition of growth was observed even at the low concentrations of nickel, cadmium and lead (15 mg/liter). The amount of metal accumulation in the fungal mycelium also increased with the increase of the metal concentration in the culture medium. In this research, for the first time, the growth parameters and the amount of accumulation of heavy metals in axenic conditions have been investigated and described.
Abdollah Beyk-Khormizi, Siavash Hosseini Sarghein, Mohammad Reza Sarafraz-Ardakani, Seyed Mohammad Moshtaghioun, Seyed Mousa Mousavi-Kouhi,
Volume 10, Issue 2 (9-2023)
Abstract
Fennel is a medicinal plant; all of its parts were being used by humans in different ways. This plant is relatively sensitive to salinity. A factorial experiment as a randomized complete block design with three replications at the greenhouse level was executed to investigate the effect of vermicompost on the increase of salinity tolerance in four fennel populations (Mashhad, Urmia, Shiraz, and Bushehr) in the vegetative stage of the plant, emphasizing the evaluation of some osmotic and antioxidant protection indicators. Experimental treatments were designed with four levels of salinity (0, 40, 80, and 120 mM of NaCl) and two levels of vermicompost (0 and 5% v/v). After harvesting, the roots were separated from the stem to analyze the biochemical variables. Salt stress caused a decrease in the total soluble sugar and starch content in the shoot and an increase of those in the root of the studied populations. In addition, under stress conditions, the proline content of shoot and root, total free amino acid, total phenol, and activity of guaiacol peroxidase and catalase were increased in fennel populations, while total soluble protein and anthocyanin content were decreased. Vermicompost treatment increased the content of soluble carbohydrates, soluble protein, free amino acids, proline, total phenol, and anthocyanin, and decreased the activity of guaiacol peroxidase in the shoot, as well as the starch content in the roots of fennel populations under non-stressed and stressed conditions. Despite observing the complexity in the changes of the analyzed indicators which were dependent on the type of population and the dose of stress, our results showed that the application of vermicompost with a concentration of 5% can improve the osmotic and antioxidant protection in the studied populations of fennel under salinity stress.
Mostafa Hosseinpour, Mohammad Mohammadi, Gholam Reza Ghezelbash,
Volume 10, Issue 3 (12-2023)
Abstract
Biological cementation is a new process in which urea hydrolysis bacteria or free urease enzyme decompose urea and increases the pH of the environment and chemical interactions in the presence of calcium ions to form calcite. Nowadays, nano-calcite is widely used in engineering, such as increasing the strength of soil and concrete, as well as in medicine, such as drug delivery and cancer treatment. This study aimed to investigate the laboratory conditions for producing nano-calcite particles with appropriate quality, size and purity by Sporosarcina pasteurii enzyme extract for use in medical and engineering studies. This investigation aimed to make calcite by S. pasteurii enzyme extract and optimize influential factors in calcite production. For this purpose, the bacterium S. pasteurii was cultured in nutrient broth containing urea and nickel, and upon reaching the appropriate time, the cells were separated and washed. Then, their enzyme extract was prepared by sonication, and calcite precipitation was studied in different amounts of urea, calcium chloride, enzyme and temperature. The quality of produced calcite crystals and their ratio compared to other crystals were investigated by XRD and SEM analyses. According to the results of XRD analysis, it was found that in 0.5 M urea and 0.25 M calcium chloride, the highest amount of calcite is produced with 96%, and the least side products are produced. Examining the particle size histogram in the sample containing 0.5 M urea and 0.25 M calcium chloride revealed that the range of particles were between 50 and 100 nm. The nature and type of crystals were studied by electron microscopy, and EDX analysis showed the presence of calcium, oxygen, and carbon. According to the results, it was found that by the increase of the concentrations of urea and calcium, the range of particle size became larger. Also, the percentage of calcite produced in low urea and calcium chloride concentrations is higher than those in high concentrations.
Siamak Yari, Sayeed-Hikmatullah Anis, Mohammad-Hossein Mohammadi-Mahdiabadi-Hasani,
Volume 10, Issue 3 (12-2023)
Abstract
Cisplatin (CP) is one of the most effective chemotherapeutic drugs, however, its clinical use is limited due to possesing nephrotoxicity, hepatotoxicity and hemotoxicity side effects. α-lipoic acid (ALA) is known as a powerful antioxidant with anti-inflammatory properties. The aim of this study was to evaluate the effect of ALA on cisplatin-induced hemotoxicity. In this experimental study, 35 NMRI mice were divided into five groups (7 mice/group) of Group 1 (Control), group 2 (CP), group3 (CP+ALA low dose), group 4 (CP+ALA high dose) and group 5 (ALA). ALA was administrated orally for 7 consecutive days and CP was injected intraperitoneally. At the end of the experiment, blood samples were collected and blood parameters were analyzed. Spleens belong to different groups were evaluated histologically. The data were revealed that erythrocyte count, White blood cells count and hemoglobin level were increased significantly in group2 in comparison with other groups. However, ALA administration improved CP induced changes in blood parameters. CP treatment induced histological changes such as increase in megakaryocytes count and accumulation of hemosiderin and collagen in red palp of spleen. ALA treatment improved CP induced histological alteration in groups 3 and 4. Results of the present study showed that ALA exerts protective effects on CP-induced hemotoxicity and histological alteration of spleen.
Rana Valizadeh Kamran, Lamia Vojodi Mehrabani, Ali Abdoulzadeh Fard, Dr Alireza Tarinejad,
Volume 10, Issue 3 (12-2023)
Abstract
Rana Valizadeh Kamran1, Lamia Vojodi Mehrabani2, Ali Aryan1 & Alireza Tarinejad1
1
Corresponding author: Rana Valizadeh Kamran, rana.valizadeh@gmail.com
Abstract. Bioremediation is a promising strategy to reduce the concentration of heavy metals that their increase in the soil was the result of the development of industries and factories in the area, threatening the environment and human health. To investigate the effect of the heavy metal chromium and the reduction of its toxic effects by bacteria (at two levels of the absence of bacteria and the presence of bacteria in Hoagland's solution), a factorial experiment was conducted as a completely randomized design with three replications and the morphological, physiological traits and plant elements were measured in the applied treatments. The results showed that the experimental treatments did not affect plant yield traits, fresh weight, stem length, and leaf length. Leaf width, chlorophyll a, b, and plant phosphorus content decreased under chromium stress and increased with bacterial treatment. Hydrogen peroxide, malondialdehyde, ascorbate peroxidase, superoxide dismutase, catalase, proline, solid soluble substances, phenol, flavonoid, and anthocyanin, as well as the content of plant elements such as chromium, nitrogen, and potassium, increased due to the chromium treatment. Using bacteria in the culture medium containing chromium, significantly decreased the hydrogen peroxide and malondialdehyde, indicating a reduction in the oxidative stress. The non-enzymatic and enzymatic antioxidants of plats in the bacterial treatments increased, indicating bacteria's role in strengthening the plant's antioxidant system. The chromium content of the plant decreased after the use of bacteria. The results showed the positive effect of using chromium-purifying bacteria in the environment of plant cultivation in reducing the harmful effects of chromium heavy metal stress.
Ali Jahangiri Zarkani, Mousa Keshavarz, Adnan Shahdadi,
Volume 10, Issue 3 (12-2023)
Abstract
Barnacles are bottom-dwelling crustaceans that live on a variety of substrates, including rocks, mollusk shells, corals, sponges, mangrove roots and leaves, and the body surface of turtles and whales. The present study deals with the species Microeuraphia permitini on the shores of Bandar Abbas in two stations of mangrove forest and rocky beach in a period of two months, and has investigated the morphometrics of the specimens, while considering the effect of the lunar cycle on reproduction, temperature and wind speed of the environment. Five longitudinal parameters (height, basal length and width, opercular length and width) were recorded. The results showed that 90% and 70% of the samples of both stations had eggs in the second day (new moon) and eighth days of lunar months, respectively, and in the twelfth day of the lunar month (full moon) all samples contained eggs. The results showed that there is a significant difference between the height and base length of the barnacle, but the difference between the opercular length, opercular width and base width is not significant. In addition, in both of mangrove and rocky stations, there was the highest correlation coefficient between opercular length and width (r=0.84 and r=0.78, respectively). The lowest correlation coefficient in mangrove station was between base length and opercular width (r=0.5) and in rocky station between height and base width (r=0.2). The wind pattern was variable during this period and the approximate decrease of the north wind was seen during the sampling period. Air temperature changes were seen between minimum 30 °C and maximum 39 °C. In addition to the tidal cycle, wind and temperature can affect the spawning of the species as an environmental stress factor.
Zeinab Mollaie, Leila Karami, Elham Rezaee, Gilda Karimi,
Volume 10, Issue 3 (12-2023)
Abstract
It has been found that the second isoform of COX enzyme known as COX-2 plays an important role in inflammation and rheumatoid arthritis and osteoarthritis. Thus, designing COX-2 inhibitors to treat inflammation is among the most important goals of researchers. In this study, the inhibitory effect of 3 new imidazole derivatives on COX-2 was evaluated by in silico approach. Molecular docking was done using Autodock Vina and the best binding mode of inhibitors was used as input of molecular dynamics (MD) simulation. MD was performed using Gromacs software for 120 ns. Then, structural and thermodynamic analyzes (ΔGbinding) and prediction of physicochemical properties were performed. RMSD data showed the compounds reached a good equilibrium and had favorable stability during simulation. Also, the RMSF showed that due to binding of inhibitors, the fluctuations of complexes decreased and the active site residues had the lowest amount. Rg, SASA and DSSP analysis showed that the protein structure did not change significantly. It was also found that Ser530 and Tyr355 residues play a more effective role in hydrogen bond formation. Physicochemical parameters determined the good drug-likeness properties for all compounds. Structural and thermodynamic analyzes (MM-PBSA) and IC50 data indicate the favorable inhibitory effect of compound 5b.
Atiqullah Sultani Ahmadzai, Hamid Ejtehadi, Mohammad Farzam, Maral Bashirzadeh, Farideh Attar, Mohammad Reza Joharchi,
Volume 10, Issue 3 (12-2023)
Abstract
The new species Onosma nuristanica is described and illustrated here on the basis of specimens collected from Parun in Nuristan and Chapa Dareh in Kunar, one of the eastern provinces of Afghanistan. An extensive review of the literature and a comparative study with specimens from several herbaria led to the determine of this new species. Our results represent significant divergence between studied specimens in terms of the most important morphological features, i.e., flower color, pedicel length, bract length filaments length, cauline shape and form. In this article, a detailed description of the new species and a diagnostic key for Onosma species thriving in Afghanistan are provided and the new species is compared morphologically with its closely related species. In addition, a distribution map is provided for the new species and its close relatives in the area.
Dr Seyed Reza Hashemi, Eng Negin Akhondpor, Dr Ayoub Farhadi, Eng Elnaz Arabiyan,
Volume 10, Issue 4 (3-2024)
Abstract
The aim of this study was to investigate the effective genes on apoptosis (BAX and Bcl2) in liver and intestinal cells of broiler chickens fed silver nanoparticles coated on clinoptilolite under acute heat stress induction. 450 d old broiler chicks (Cobb 500) were used in five treatments and six repetitions, and 15 pieces were used in each experimental unit in the form of a completely random design. Experimental diets were: 1) control or basal diet 2) basal diet supplemented by 1% clinoptilolite 3) basal diet supplemented by 1% clinoptilolite coated with 0.5% nanosilver 4) basal diet supplemented by 0.15% organic acid and 5) basal diet supplemented by 1% clinoptilolite coated with 0.5% nanosilver and 0.15% organic acid. Silver nanoparticles coated on clinoptilolite were investigated using XRF and FTIR techniques. In order to induce heat stress, the birds were affected by heat stress for one week in the last week of the breeding period, and on the last day of the stress, liver and intestine samples were obtained to check gene expression. The results of this experiment show that the treatments of clinoptilolite and silver nanoparticles coated on clinoptilolite have an increasing effect on the expression of Bcl2 and Bax, while this effect was not seen in the organic acid treatment. In conclusion, it could be said that if silver nanoparticles are used in feeding livestock and poultry, it is better to use organic acid supplements to reduce the side effects of silver nanoparticles.
Elham Ahmadi, Nasim Aghaei Delche,
Volume 10, Issue 4 (3-2024)
Abstract
Continuous environmental monitoring is one of the important principles of management and is environmental assessment axis, and molluscs are one of the biological indicators of ecosystem quality. For this purpose, snails and slugs were collected and identified in the east of Tehran province which during it according to the availability, habitat diversity and geomorphological differences in more than 3251 samples from five microclimates were collected and identified. Specimens were gathered by hand, sweeping net and other techniques from aquatic plants, rotten underwood, shrubs and weeds, their geograical and regional properties were recorded and transfered to laboratory. Then through available morphological identification keys, specimens were identified as 19 species belonging to 13 genera and 10 families based on morphometric characteristics, including (shape and size of operculum, aperture, dextral or sinistral profile of their shell, pneumostome, keel, radula, renal ridge and reproduction system) and the comparison of photos and the identification key of these taxons were prepared and discussed:
Bithynia tentaculata (Linnaeus, 1758), Physella acuta (Draparnaud,1805), Lymnaea truncatula (Müller, 1774), , Lymnaea palusris (Müller, 1774) , Lymnaea pereger (Müller, 1774) , Lymnaea gedrosiana (Annandale and Prashad, 1919), Planorbis planorbis (Linnaeus, 1758), Gyraulus euphraticus(Mousson, 1874), Gyraulus convexiusculus (Hutton, 1849), Succinea putris (Linnaeus, 1758), Helicella krynickii (Krynicki, 1833), Helicella derbentina(Krynicki, 1836), Caucasotachea atrolabiata (Krynicki, 1833), * Monacha cartusiana (Müller, 1774), Oxychilus herzi (Boettger, 1889), Agriolimax agrestis=Deroceras reticulatum (Müller, 1774), *Theba pisana (Müller, 1774), Parmacella ibera Eichwald, 1841, Planorbis carinatus Müller, 1774.
Two species marked with an asterisk are new records for Iranian mollusc fauna. In this study, among the samples collected, the largest families in terms of species diversity are Planorbidae with four species (Planorbis planorbis, Gyraulus euphraticus, Gyraulus convexiusculus, Planorbis carinatus), Lymnaeidae with four species (Lymnaea truncatula, Lymnaea gedrosiana, Lymnaea palustris, Lymnaea pereger) and Helicidae with four species: Helicella krynickii, Helicella derbentina, Caucasotachea atrolabiata, Theba pisana. The highest and lowest species diversity among the identified species with 18 and 5 species are related to stations 4 and 5 of the investigated areas respectively. The whole collection will be kept permanently in the Zoology Museum of Iranian Research Institute of Plant Protection.
Miss. Hanieh Ghandchi, Mrs. Reihaneh Ramezani, Mrs. Zahra Moosavinejad,
Volume 10, Issue 4 (3-2024)
Abstract
Recently, milk exosomes have attracted much attention from researchers due to their availability and efficiency in cosmetic products and also as drug delivery nanocarriers. Since it is very important to find a simple and efficient method to purify these vesicles, in this research some methods of exosome isolation from bovine milk such as ultracentrifugation, using PEG polymer and several commercial kits were discussed and characterized. Detection of exosomes has been done using DLS and electron microscopy.
In ultracentrifugation, as the most common method of exosome isolation, the number of particles in the electron microscope images was estimated to be very low (5 ± 2 particles), while in the microscopic images of the Exosun kit, a large number of exosomes (150 ± 30 particles) was visible. In PEG precipitation, the average diameter of the particles in DLS results was 263 nm and more than the ultracentrifugation, Exocib and Anaexo kit, where the diameter of the particles was 176 nm, 142 nm, and 123 nm, respectively. The average diameter of the particles in the microscopic images of the Exosun kit was 30-70±10 nm, and DLS results confirmed the small size of the isolated particles. Considering the large number of small particles (≥ 30nm) in the microscopic results of the exosun kit, other methods may not have been able to isolate these small particles. Finally, although all the studied methods were able to isolate exosome from milk, more extensive studies are necessary to make a more accurate comparison and to introduce a standard method for isolating exosome from bovine's milk.
Narjes Rezazadeh Moghadam, Asghar Zamani,
Volume 10, Issue 4 (3-2024)
Abstract
The family Verbenaceae, currently consists of Verbena and Phyla genera in different parts of Iran, especially northern provinces. The frame of this family has undergone significant changes in comparison with the previous references. For example, Vitex and Clerodendrum have been defined as the genera of this family in previous references. Accordingly, in this study, the relationship among these four genera has been evaluated using morphological and leaf anatomical characters of 20 samples. For this purpose, 67 qualitative and quantitative morphological and anatomical traits were used. The analysis of data was performed using R software ver. 4.3.1. For simultaneous analysis of quantitative and qualitative data, Factor Analysis of Mixed Data (FAMD) method was applied. The results of this study indicate the high value of some quantitative traits such as main vein xylem length, seed width, style length and blade width and some qualitative traits such as blade epidermal cells shape, stomata position, two arms of blade position in relation to each other, indumentum type, main vein epidermal cells shape, petal color, blade vascular bundles number, density of indumentum, leaf margin shape and stem branches length in the clustering of the genera. Totally, Analysis of data led to the separation of these genera. In accordance with the phylogenetic studies, Vitex and Clerodendrum show more affinity to each other and are separated from the currently native members of Verbenaceae in Iran, i.e. Verbena and Phyla.
Fatemeh Jafari, Ali Ganjeali, Elham Amjadi,
Volume 10, Issue 4 (3-2024)
Abstract
Nepeta binaludensis jamzad, as a medicinal plant, which is at risk of extinction due to irregular harvesting and damage of its habitat. Four treatments including: 1- inoculation with Azotobacter chooroccum, 2- inoculation with Bacillus cereus and Pseudomonas putida, 3- inoculation with a mix of three bacteria (A. chooroccum + B. cereus + P. putida) and 4 controls (without inoculation), in the form of a completely randomized design with 3 replications in two vegetative growth stages (ten and twenty weeks after sowing). Plant growth-promoting bacteria (PGPB) improved all the evaluated morphological characteristics, including the length and dry weight of the aerial part, total leaf area and root length compared to the control. Also, the results of biochemical investigations showed that in 10 and 20 weeks old plants, the application of PGPB could increase the phenolic and flavonoid compounds and also antioxidant activity. The contain of some elements such as calcium, potassium, phosphorus, iron and magnesium also increased as a result of inoculation with PGPB. Since the PGPB improved the growth and also increased the effective compounds of N. binaludensis plants, they can be introduced as useful bio fertilizers and considered as a good alternative to chemical fertilizers.
Dr Fateme Mousavi,
Volume 10, Issue 4 (3-2024)
Abstract
Quinoa seed (Chenopodium quinoa) is considered a unique food source due to its rich protein content and high antioxidant activity related to polyphenols. In the present study, with the aim of selecting the quinoa seeds to send to space, the response of protein content, phenol, flavonoid, antioxidant capacity, and the germination index of its seeds to simulated vacuum conditions of space was evaluated. The results showed a significant increase in the seed germination index for the vacuum-treated group compared to the control group. Total phenolic and flavonoid content was higher in vacuum-treated seeds compared to the control group. Vacuum conditions significantly increased the antioxidant capacity of quinoa seeds. The total seed protein content in the vacuum-treated and control groups was 25 and 35 mg/ml, respectively. The seed protein profile showed 13 distinct protein bands in the molecular weight range of 15 to 70 kilodaltons. The intensity of protein bands was significantly different between vacuum treatment and control groups. Structural changes in the seed pericarp as well as water and oil exit from the seeds under vacuum conditions can be the causes of different biochemical and physiological responses of quinoa seeds in the present study.
Saeideh Khamushi, Fatemeh Nejadhabibvash,
Volume 11, Issue 1 (6-2024)
Abstract
The aim of this study was to evaluate the changes in the essential oil chemical composition of Satureja bachtiarica Bunge were inoculated by spores of the arbuscular mycorrhizal inoculation Glomus caledonium and foliar application of zinc chelate levels (0, 2, 4 and 8 g/L) in 2019, at Zarrin Giah greenhouse of Urmia city in West Azerbaijan Province, in a randomized complete block design with three replications. Plants were harvested at full flowering stage. Essential oils were obtained by Clevenger apparatus and were analyzed by GC and GC/MS. Comparison of means by Duncan's multiple range test showed that inoculation with Glomus caldonium had no significant effect on the essential oil yield of Satureja bachtiarica flowering shoots, but the foliar application of zinc chelate had statistically significant effect. However, there was no difference among different concentrations of zinc chelate in terms of effect on essential oil yield. The treatments also changed the essential oil composition compared to the essential oil of the control group. These changes included the reduction of beta-caryophyllene, sabinen hydrate, borneol and carvacryl acetate compounds and the increase of citral and octyl phthalate in the plant inoculated with mycorrhiza, as well as the increase of carvacrol in the essential oil of plants treated with zinc chelate. Also, inoculation with mycorrhizal fungi and zinc chelate foliar application increased the amount of sesquiterpene & diterpene and monoterpene compounds, respectively.
Dr. Atta Mouludi-Saleh, Dr. Soheil Eagderi, Dr. Hadi Poorbagher, Dr. Hamid Reza Esmaeili,
Volume 11, Issue 1 (6-2024)
Abstract
This study was conducted to investigate the ecological niche overlap of two sympatric species, Paracapoeta trutta and Capoeta damascina in the Iranian part of the Sirvan River, Persian Gulf basin. For this purpose, during the winter of 2021, sampling was done from eight stations (each with three repetitions). After identification, samples were released into the same sampling sites. Then some 10 environmental factors including depth, water velocity, river width, pH, temperature, electrical conductivity (EC), total dissolved solids (TDS), Altitude, slope, and dissolved oxygen were measured and recorded. Investigating the habitat suitability indices of P. trutta were recorded as pH 7.5-8, temperature about 10°C, TDS 150mg/liter, EC 400μS/cm, altitude 1150 m, depth 15cm, river width about 9m, velocity 1 m/s, slope 2% and dissolved oxygen 9mg/l and for C. damascina as pH 6-6.5, temperature about 10°C, TDS 180 to 200mg/l, EC 300μS/cm, altitude 1150m, depth 25cm, river width about 6m, velocity 0.5 m/s, the slope is 2% and the dissolved oxygen 8mg. The results of the ecological niche overlap showed that two species based on studied environmental factors had the highest overlap value for dissolved oxygen (0.646) and the value for temperature (0.293). The average ecological niche overlap was 0.432 for the two species.
Mr Ali Alimoradi, Dr Jamileh Salaramoli, Dr Mahsa Daneshmand, Dr Raham Armand,
Volume 11, Issue 1 (6-2024)
Abstract
Calyx of eggplant is recognized as agricultural byproducts containing significant levels of phenolic, flavonoid, and anthocyanin compounds that facilitate the reduction of silver ions, enabling the eco-friendly production of silver oxide nanoparticles through a green synthesis approach. The utilization of silver oxide nanoparticles has garnered considerable interest in various industries due to their versatile applications and environmentally sustainable nature. Various analytical techniques such as ultraviolet- visible spectrophotometry, X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and dynamic light scattering were employed to confirm the formation of silver oxide nanoparticles and characterize their properties. The antioxidant, antibacterial, and antifungal activities of the silver oxide nanoparticles and the aqueous extract of calyx of eggplant were evaluated through assays including 1,1-diphenyl-2-picrylhydrazyl radical scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical inhibition, disc diffusion, minimum inhibitory concentration and minimum bactericidal concentration. The absorption peak at 422 nm confirmed the presence of silver oxide nanoparticles, with a particle size of approximately 140 nm determined by DLS analysis and a spherical shape observed through SEM imaging. The biological activities of the silver oxide nanoparticles, including their antioxidant properties and antimicrobial effects were found to be superior to those of the aqueous calyx of eggplant extract.
Mrs Nasrin Mehdinejhad Moghadam, Dr Omid Sofalian, Dr Naser Zare, Dr Mohamad Sedghi, Dr Rahmatollah Karimizadeh, Dr Fatemeh Mohamadiazar,
Volume 11, Issue 1 (6-2024)
Abstract
Legumes are common crops in arid and semi-arid regions. This study was conducted to investigate three levels of salinity stress on 18 lentil cultivars in the greenhouse and laboratories of Mohaghegh Ardabili University in 2016-2016 in a factorial manner in the form of a randomized complete block design with three replications. The results of comparing average lentil genotypes were done with Duncan's method at the five percent probability level. Correlation was done in separate stress levels. The dendrogram of cluster analysis divided the genotypes into three groups in control and 60 mM conditions and two in 120 mM conditions. Genotypes 7 and 5 were in the top group in all groupings. The relationship between ISSR molecular markers and physiological traits was calculated among genotypes. All traits in three levels of salinity stress had a significant correlation with some ISSR markers. A total of 22 positive markers for physiological traits were identified at the control level, 9 positive markers at the moderate stress level, and 23 positive markers at the severe stress level. Among the 21 studied ISSR primers, the P8A11 marker had the largest number of gene loci related to the studied physiological traits.
Bahare Asgari, Dr Ali Mohammadi, Dr Bahare Attaran,
Volume 11, Issue 1 (6-2024)
Abstract
The aim of the present work was to produce a stable nanoemulsion containing clove (Syzygium aromaticum) essential oil (PCLO) and evaluate its antimicrobial activity. The effect of the preparation technique, the type of surfactant and the ratio of surfactant to oil was evaluated to optimize the preparation formula of oil-in-water nanoemulsion. The optimized formula prepared by low energy production method containing 4% w/w PCLO and 12% w/w mixed surfactant (SDS + Tween 80) produced a clear and stable nanoemulsion for 90 days with an average particle diameter below 150 nm. The antibacterial activity of pure PCLO and its nanoemulsions (NCLO) was investigated by disk diffusion, agar well, and broth methods in 3 indicator bacteria of gastrointestinal infections, Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium. Minimum inhibitory concentration (MIC) and bacteriocidal concentration (MBC) as well as dynamic killing time were determined in the tested bacteria. Strong antibacterial activity of PCLO and NCLO was revealed in the concentration range of 1000-2000 ppm. The killing kinetics study showed that during the first 15 minutes of exposure to NCLO at the MIC concentration, there was a rapid and extensive reduction in the amount of viable microorganisms. The presented data, considering the optimal performance of antimicrobial substances in food, cosmetics and chemical industries, can help in the rational design of nanoemulsion-based essential oil delivery systems.
Dr Nader Chaparzadeh, Dr Leila Zarandi-Miandoab, - Mina Ali-Pashaei Dehkhargani,
Volume 11, Issue 1 (6-2024)
Abstract
Texture quality is an important factor in evaluating of fruits. The cut surfaces of the apple fruit turn brown because of the oxidation of phenolic compounds, triggered by polyphenol oxidase enzyme. Preventing the oxidation of phenolic substances and changing color of fruit are very important in the food industry. Due to the adverse effects of chemical additives on human health and increasing consumer preference for natural alternative compounds makes an interesting market for natural plant ingredients. This study was conducted to investigate the effect of some chemical and natural compounds including sodium metabisulfite, and citric acid, aromatic waters (sweats) of chicory, licorice and lemongrass on polyphenol oxidase enzyme activity of apple fruits. The activity of polyphenol oxidase was evaluated spectrophotometrically using pyrogallol as substrate. The optimum temperature and pH values were 32 °C and 7, respectively. It was found that the enzyme activity decreased due to use of natural compounds, sodium metabisulfite and citric acid. In conclusion, polyphenol oxidase activity can be reduced to prevent of fruits browning by using suitable natural compounds instead of chemicals.
