Nasrin Farasat, Masoud Sheidai, Hossein Riahi, Fahimeh Koohdar,
Volume 9, Issue 3 (12-2022)
Abstract
The genus Ulva L. (Sea lettuce), one of the most valuable marine macroalgae, is found in shallow coastal areas and in brackish and fresh water around the world. Ulva species have nutritional and medicinal values in addition to other applications, and contain various compounds such as lipids, proteins and carotenoids. These species have high phenotypic plasticity and varied in response to different environmental conditions. In this study, 38 populations of 10 species of Ulva from the coastal areas of the Persian Gulf and Oman Sea, from different areas of Bushehr, Hormozgan and Sistan and Baluchestan provinces, were studied and identified on the basis of morphological and anatomical traits. Different populations showed variations in characteristics such as height, shape and color of thallus, number of pyrenoids and cell dimensions. Metabolites such as total protein, total oil, chlorophyll a, b and carotenoids contents were measured and compared among the different populations. The results should that the percentage of protein was highest in U. prolifera of Shif Island from Bushehr province as compared with the Ulva species studied, with about 26.5% of dry weight, and the percentage of total oil was lowest in U. flexuosa of Hakhamanesh region from Hormozgan province as compared with the species studied, with about 4.8% of dry weight. These results show that different environmental conditions affect the morphological and anatomical structure of species and the content of their metabolites. Due to the high-protein and low-calorie content of the Ulva genus and the importance of finding new and sustainable resources for food and pharmaceutical industries, the species of this genus have valuable potentials.
Fatemeh Khakdan, Athar Sadat Javanmard,
Volume 9, Issue 3 (12-2022)
Abstract
Basil is an important medicinal plant, belonging to the Lamiaceae family. Basil is used for the treatment of different diseases such as stomachache, headache, constipation, fever and infections, as well as to reduce and regulate the blood sugar. Moreover, Basil is known for its antibacterial, antifungal and antioxidant properties. In this study, the impact of drought stress on phenols and flavonoids concentrations, as well as the antioxidant activities of leaf extract of three basil cultivars were investigated. Seeds of basil cultivars (mikhak, green, purple) were cultivated in sand-loamy soil and drought stresses (75%, 50% and 25% of the field capacity) were applied on six-leave plants. Experiments were conducted in a completely randomized design and three repeats. Total phenols, total flavonoids and flavonols concentrations of basil leaf extracts and their antioxidant activities were measured. The maximum values (with significant differences) of three groups of non-enzymatic antioxidants and the highest levels of antioxidant activities were observed for green cultivars under mild stresses (75% of the field capacity). It was observed that antioxidant activities were elevated by the increase of the concentration of the plant extract. Drought stress results in oxidative stress in basil plants. Phenols, flavonoids and flavonols are well-known as strong antioxidants have a role in the plant’s protection against the oxidative stress. In thrice cultivars of basil which were investigated, concentrations and abilities of these compounds to inhibit free radicals were decreased by the increase of the levels of drought stresses. It seems that both basil genotypes and drought stress levels affected the production of antioxidants studied and, consequently, have impacts on the plant inhibitory abilities against the oxidative stress.
Asghar Zamani,
Volume 9, Issue 3 (12-2022)
Abstract
The seed micromorphological characters of 15 taxa of Iranian Pyrus comprising 22 individuals were examined by Scanning Electron Microscopy (SEM) and Digital Microscopy (DM) for the first time in this survey. For the assessment of probable ecological effects, several individuals of some widespread species were studied. After collecting several samples of each wild taxa of Pyrus from different localities in a ten-year period, the specimens were identified by means of the examination of type specimens and different Floras. After the examination of the seeds surface, qualitative (e.g., shape and ornamentation of seeds including areolate, foveate, reticulate, colliculate, striate and undulate types) and quantitative characters (e.g., size of seeds, size of surface cells and thickness of cell walls) were assessed. Statistical analyses were performed on the data using SPSS software. According to these observations, seed morphological characters showed to have a considerable variation even among the different individuals of the same species, verifying complexity of the genus as revealed by the different approaches which were previously reported. However, some descriptive characters of seeds such as shape, size, surface ornamentation type, cell size and cell wall thickness are presented for the first time for the genus.
Raheleh Ahmadpour, Roya Ghalavand, Saeed Reza Hosseinzadeh, Nezam Armand,
Volume 9, Issue 4 (3-2023)
Abstract
Successful germination is the main factor in proper establishment and seedling yield. Information on seed germination, in the face of temperature changes at the time of planting, is a way to identify more suitable cultivars for cultivation at the planting site. For this purpose, a factorial experiment was performed based on completely randomized design with 3 replications. This experiment was conducted to investigate the temperature changes on the germination and growth indicators of lentil cultivars at Khatam Al-Anbia University of Behbahan. The studied treatments included five lentil cultivars of Gachsaran, Kimia, Robat, Ziba and Bilehsevar, and the temperature levels were 20, 25 and 30 °C, similar to the autumn conditions of Khuzestan Province. The results showed that all the seedlings of investigated cultivars cultivated at temperatures of 20 and 30 °C had a significant decrease in all the investigated traits, i.e., germination percent, germination rate, germination vigor, seed vigor index, endosperm consumption, plumule length, radicle length, seedling length, plumule dry weight and radicle dry weight, in comparison with those seedlings cultivated in 25 °C. The study of the interaction of cultivar-temperature effects showed that Bilehsevar cultivar had the highest germination and growth indices at 25°C compared to other investigated cultivars. This increase in traits such as germination rate, germination vigor, seed vigor index, plumule dry weight and radicle length was more significant in Bilehsevar cultivar compared to other cultivars. The second rank of the highest germination and growth indices was assigned to Gachsaran cultivar under 25°C conditions, which had a significant increase compared to other cultivars (Kimia, Robat and Ziba). Considering the autumn cultivation of lentils and the changes of temperature in that season (temperature range of 20 to 30 °C) in the studied area, the use of Bilehsevar and Gachsaran cultivars under the temperature of 25 °C, due to their higher germination indicators compared to the other cultivars, i.e., Ziba, Kimia and Robat, could be recommended.
Shiva Tabatabaie Roodsati, Alireza Iranbakhsh, . Mansoureh Shamili, Zahra Oraghi Ardabili,
Volume 9, Issue 4 (3-2023)
Abstract
Selenium, a non-essential element for plants, is essential for animals as well as human beings. Although the role of selenium in plants is yet to be properly understood, previous researches have shown that this element can affect plant growth and metabolism. In this study, the effect of foliar application of selenium nanoparticles (0, 5, 10, and 20 mg/L) and sodium selenate (0, 5, 10, and 20 mg/L) on the physiological and biochemical responses of bell pepper (Capsicum anumm L.) was investigated. The potential changes in various growth and biochemical indices were evaluated in response to the treatments. According to the results, selenium treatments at concentrations of 10 and 20 mg/L reduced the biomass accumulation in both roots and shoots. These treatments also increased the content of hydrogen peroxide and malondialdehyde. The foliar application of selenium led to the increase of the concentrations of soluble phenols, proline and thiols. The activity of antioxidant enzymes including catalase, peroxidase, ascorbate peroxidase, and polyphenol oxidase were increased in response to the selenium treatments. The protease activity displayed a similar upward trend following the selenium treatments.
Vajiheh Ganjeali, Monireh Cheniany, Parissa Taheri, Maryam Mazaheri-Tirani,
Volume 10, Issue 1 (6-2023)
Abstract
Wheat (Triticum aestivum L.), as one of the most important cultivated crops in the world and Iran, is constantly threatened by many diseases, including Fusarium contamination. Due to the unique characteristics of nanoparticles, copper oxide nanoparticles show high antibacterial and antifungal properties. The purpose of this research was to comparatively investigate the antifungal effects of copper oxide nanoparticles and its bulk form on the suppression of Fusarium calmorum in wheat. For this purpose, a pot experiment was done with four levels (10, 250, 500, and 1000 mg L-1) of nanoparticles and bulk form of copper oxide treatments in wheat (Roshan cultivar). The results showed that the maximum inhibition of root pathogenicity was observed in a high concentration of nanoparticles as compared to bulk form. The 250 and 500 mg L-1 concentrations of copper oxide nanoparticles caused the highest stem and root length and the highest dry weight of the aerial part and root, respectively. Treatment with 10 and 250 mg L-1 nanoparticles also increased the content of chlorophyll a, total chlorophyll, carotenoid, chlorophyll stability index, membrane stability coefficient, and relative leaf water content. While the content of chlorophyll b, malondialdehyde, hydrogen peroxide, and proline increased with the increasing concentration of both nanoparticle and bulk forms. The results of this research showed that the low and medium concentrations of nanoparticles were more successful in inhibiting the aforementioned fungus than the bulk form.
Sarehi Ebrahimi, Seyed Mehdi Razavi, Mansour Afshar Mohammmadian,
Volume 10, Issue 1 (6-2023)
Abstract
The increase of the entry of industrial effluents containing various types of heavy metals into water and soil leads to the ever-increasing spread of environmental problems. Therefore, it is necessary to find less expensive solutions to reduce the pollution. In this regard, in order to compare the effect of the removal of heavy elements on some of the morphological and physiological characteristics of C. alternifolius, Ch. zizanioides and Aloe vera plants, an experiment was conducted in the form of a randomized complete block design with three replications for 14 months at the University of Guilan. The results showed that under the treatment of industrial wastewater, the metal accumulation indices (MAI) of zinc, chromium, lead, copper, manganese, nickel, magnesium were higher in Ch. zizanioides and Aloe vera than those in C. alternifolius. In all three studied plants, the indices of root length, shoot length, total biomass and tolerance index (TI) had a significant decrease compared with those in the control plants, and Ch. zizanioides and A. vera plants had the lowest percentage of decrease. In addition, other investigated components including absorption index (UI), total soluble sugar, total phenol content, total flavonoid, total anthocyanin, total tannin, and DPPH free radical inhibition percentage increased in all three plants under industrial wastewater treatment. The highest percentage of increase in all investigated components, except DPPH free radical inhibition percentage, was observed in Ch. zizanioides. In general, it can be said that in terms of MAI and morphological and physiological characteristics, Ch. zizanioides and A. vera were more successful than the C. alternifolius. Therefore, it is recommended to plant the studied plants in the order of priority (Ch. zizanioides ˃ A. vera ˃ C. alternifolius) in soils contaminated with industrial effluents, in order to remediate the soil.
Azarnoosh Jafari, Ameneh Assadi Barbariha, Fereshteh Ghasemzadeh,
Volume 10, Issue 1 (6-2023)
Abstract
The present research, a floristic study was executed on Imamverdy, Dartum, Ghapagh, Garivan and Niestaneh villages, located 30 km south of Bojnourd (North Khorassan province) with altitudinal range of 1442-1783 m. The present study aimed to identify the specimens, their possible medicinal properties, endemism and concervation status. For this purpose, the specimens were collected during March-November 2012 and identified using Flora of Iran and Flora Iranica. The results showed the presence of 133 species from 104 genera and 38 families, one species belonged to Gymnosperms and the rest, 132 species, belonged to Angiosperms, of which five families, 13 genera and 18 species were monocotyledons and 32 families, 90 genera and 114 species were dicotyledons. Moreover, 40 species were found to have medicinal applications in the studied region. Fabaceae, Brassicaceae, Poaceae, Asteraceae and Lamiaceae were the largest families and Astragalus, Poa, Vicia and Euphorbia were reported as the largest genera in the region. Erysimum koelzii, Astragalus khoshjailensis, Eryngium bungei, Acanthophyllum pachystegium, Sclerorachis platyrachis, Taraxacum hydrophyllum were endemic species for Iran. Astragalus ackerbergensis was found to be in vulnerable status and Rubia florida, Mentha longifolia, Fumaria vaillanti and Onopordum carmanicum were found to be at low-risk status. The maximum and minimum percentages of chorotypes were related to Irano-Turanian with 68.14% and Irano-Turanian, Saharo-Arabian regions with 0.7%, respectively. The maximum and minimum percentage of life form were reported to be hemicryptophytes with 45.8% and cryptophytes with 3%, respectively. The results reflected the cold and arid climate and uncontrolled grazing in the studied region.
Mahmoud Bidarlord, Mohamad Dehdar Dargahi, Adel Jalili,
Volume 10, Issue 1 (6-2023)
Abstract
Solanum chenopodioides is introduced here as a new record for the flora of Iran from the coastal areas of the Caspian Sea, Guilan province. This species grows in Bujagh National Land-Sea Park, on sand dunes, along with other coastal psammophytic plants. This species belongs to the Morelloid clade. It differs from the related species S. nigrum, by its shrubby nature, perfect leaves margin, dull berries, bi-colored petals in the middle, and peduncle that bends a bit downwards in fruit. This plant is native to southern South America. It is introduced as a common weed all over the world; in addition, this species is considered an alien species for the flora of Iran. Transit of goods, especially wool, to the different parts of the world is one of the ways of the spread of this species all over the world. In addition to providing some pictures of the plant, some notes about the distribution and ecology of this species were also provided in this paper. The numerous reports of alien species from Bujagh National Park in recent years could be regarded as a credible warning for the stability of native vegetation of the area and existing ecosystems.
Ali Asghar Arjmandi, Hamid Ejtehadi, Farshid Memariani, Mansour Mesdaghi,
Volume 10, Issue 1 (6-2023)
Abstract
Understanding the different patterns of plant diversity and distribution along the environmental gradients is essential for managing ecosystems, especially in fragmented habitats due to intensive human pressure. The Darkash area is located in the western Aladagh Mountains in Khorassan-Kopet Dagh Floristic Province. The easternmost patches of the Hyrcanian montane forests grow in this area. The three main vegetation types in the area are shrublands/scrub forests, oak forests (Quercus castaneifolia), and subalpine vegetation. In this study, we aimed to evaluate the plant diversity in the understory of plant communities along the elevational gradient. We sampled 187 vegetation plots (2m x 2m) along a 1300m elevational gradient (in 100-m classes) using the stratified-random method and estimated the abundance and canopy cover of the species. Floristic characteristics and different diversity indices were measured. In total, 252 plant species belonging to 161 genera and 44 families were identified. Of these, 41 species were found to be endemic or semi-endemic. Hemicryptophytes and therophytes are the dominant life-forms in the study area. The Irano-Turanian elements (46.8%) make the major chorotype in the flora of the area, followed by Bi-regional (17.5%), Tri-regional (17.5%), Pluri-regional (11.5%), and Euro-Siberian (6.7%) elements. The shrubland understory has the highest Hill's and beta diversity indices, followed by the oak forest and the subalpine understory. The biodiversity changes sinusoidally along the elevation. In order to protect plant species, especially young oak seedlings, it is recommended to create a protective corridor along the elevational gradient in this area. This strategy is essential to protect plant diversity in these habitats.
Yasaman Salmaki, Mahya Khakpash,
Volume 10, Issue 2 (9-2023)
Abstract
Stachys sect. Satureoides includes four species characterized by being annual herbs having oblanceolate leaves, urceolate calyx in fruit and dense hairs at mouth and are distributed from Eastern and Southern Turkey, Syria, Northern Iraq to Western Iran. The main aim of present study is to determine the phylogenetic placement of S. sect. Satureoides based on Stachys melampyroides, as the only representative of this section in Iran. The present molecular study was conducted based on nuclear ribosomal ITS marker, using Maximum Parsimony and Bayesian Inference analyses. In total, 86 accessions (including nine outgroups and 77 ingroups) were included. Our results demonstrated that sect. Satureoides was placed in Olisia clade which is one of the most heterogenous and taxonomically challenging clades of Stachys as a paraphyletic genus. Moreover, not only sect. Satureoides was not found to be phylogenetically close to sect. Neurocalyx, as had been suggested by previous morphological studies, but also it was placed as a sister group of sect Fragilicaulis which was characterized by chasmophilus species with fragile stems and yellow flowers. Our results revealed that sect. Neurocalyx could be the closest group to the Satureoides+Fragilicaulis clade. In addition, based on the nrITS phylogenetic tree, annual species of Stachys showed affinity to perennial Stachys species. Hence, it seems that “Annual habit” have been evolved among their perennial ancestors, independently.
Elmira Shokoohi, Omid Sofalian, Ali Asghari, Saeid Khomari, Behrooz Esmaielpour, Hamed Aflatooni,
Volume 10, Issue 2 (9-2023)
Abstract
Chickpea is one of the most important plants of the legume family and is very important in the diet. In order to investigate the genetic diversity of chickpea, an experiment was conducted with 18 chickpea genotypes in the form of a completely to investigate the genetic diversity of chickpea, an experiment was conducted with 18 chickpea genotypes in the form of a completely randomized block design. After acclimatization of plants to cold, freezing treatment was applied at temperatures of -6, -8 and -10 and their 50% lethality temperature (LT50) was determined by probit transformation. Before and after the habituation stage, a leaf sample was taken and the relative content of leaf water, photosynthetic pigments, proline, soluble sugar, protein percentage, catalase, peroxidase, polyphenol oxidase and greenness index were measured. Genotype number 5 with the lowest LT50 (-8.86) and the highest survival percentage (80%) was the most resistant genotype and genotype 10 with the highest LT50 (-3.57) and the lowest survival percentage along with genotype 15 were recognized as the most sensitive genotypes. In order to evaluate genetic diversity, DNA extraction was utilized and 21 different ISSR primers were used in the investigation. The results showed the presence of polymorphism among the cultivars studied. A total of 101 clear bands were produced, of which 94 were polymorphic bands. Polymorphic information content (PIC) was in the range of 0.332 (initiator 7) to 0.049 (initiator 16). The amount of gene diversity was between 0.126 and 0.977 changes. Cluster analysis of genotypes was done using Jaccard similarity coefficient and UPGMA method
Abdollah Beyk-Khormizi, Siavash Hosseini Sarghein, Mohammad Reza Sarafraz-Ardakani, Seyed Mohammad Moshtaghioun, Seyed Mousa Mousavi-Kouhi,
Volume 10, Issue 2 (9-2023)
Abstract
Fennel is a medicinal plant; all of its parts were being used by humans in different ways. This plant is relatively sensitive to salinity. A factorial experiment as a randomized complete block design with three replications at the greenhouse level was executed to investigate the effect of vermicompost on the increase of salinity tolerance in four fennel populations (Mashhad, Urmia, Shiraz, and Bushehr) in the vegetative stage of the plant, emphasizing the evaluation of some osmotic and antioxidant protection indicators. Experimental treatments were designed with four levels of salinity (0, 40, 80, and 120 mM of NaCl) and two levels of vermicompost (0 and 5% v/v). After harvesting, the roots were separated from the stem to analyze the biochemical variables. Salt stress caused a decrease in the total soluble sugar and starch content in the shoot and an increase of those in the root of the studied populations. In addition, under stress conditions, the proline content of shoot and root, total free amino acid, total phenol, and activity of guaiacol peroxidase and catalase were increased in fennel populations, while total soluble protein and anthocyanin content were decreased. Vermicompost treatment increased the content of soluble carbohydrates, soluble protein, free amino acids, proline, total phenol, and anthocyanin, and decreased the activity of guaiacol peroxidase in the shoot, as well as the starch content in the roots of fennel populations under non-stressed and stressed conditions. Despite observing the complexity in the changes of the analyzed indicators which were dependent on the type of population and the dose of stress, our results showed that the application of vermicompost with a concentration of 5% can improve the osmotic and antioxidant protection in the studied populations of fennel under salinity stress.
Rana Valizadeh Kamran, Lamia Vojodi Mehrabani, Ali Abdoulzadeh Fard, Dr Alireza Tarinejad,
Volume 10, Issue 3 (12-2023)
Abstract
Rana Valizadeh Kamran1, Lamia Vojodi Mehrabani2, Ali Aryan1 & Alireza Tarinejad1
1
Corresponding author: Rana Valizadeh Kamran, rana.valizadeh@gmail.com
Abstract. Bioremediation is a promising strategy to reduce the concentration of heavy metals that their increase in the soil was the result of the development of industries and factories in the area, threatening the environment and human health. To investigate the effect of the heavy metal chromium and the reduction of its toxic effects by bacteria (at two levels of the absence of bacteria and the presence of bacteria in Hoagland's solution), a factorial experiment was conducted as a completely randomized design with three replications and the morphological, physiological traits and plant elements were measured in the applied treatments. The results showed that the experimental treatments did not affect plant yield traits, fresh weight, stem length, and leaf length. Leaf width, chlorophyll a, b, and plant phosphorus content decreased under chromium stress and increased with bacterial treatment. Hydrogen peroxide, malondialdehyde, ascorbate peroxidase, superoxide dismutase, catalase, proline, solid soluble substances, phenol, flavonoid, and anthocyanin, as well as the content of plant elements such as chromium, nitrogen, and potassium, increased due to the chromium treatment. Using bacteria in the culture medium containing chromium, significantly decreased the hydrogen peroxide and malondialdehyde, indicating a reduction in the oxidative stress. The non-enzymatic and enzymatic antioxidants of plats in the bacterial treatments increased, indicating bacteria's role in strengthening the plant's antioxidant system. The chromium content of the plant decreased after the use of bacteria. The results showed the positive effect of using chromium-purifying bacteria in the environment of plant cultivation in reducing the harmful effects of chromium heavy metal stress.
Atiqullah Sultani Ahmadzai, Hamid Ejtehadi, Mohammad Farzam, Maral Bashirzadeh, Farideh Attar, Mohammad Reza Joharchi,
Volume 10, Issue 3 (12-2023)
Abstract
The new species Onosma nuristanica is described and illustrated here on the basis of specimens collected from Parun in Nuristan and Chapa Dareh in Kunar, one of the eastern provinces of Afghanistan. An extensive review of the literature and a comparative study with specimens from several herbaria led to the determine of this new species. Our results represent significant divergence between studied specimens in terms of the most important morphological features, i.e., flower color, pedicel length, bract length filaments length, cauline shape and form. In this article, a detailed description of the new species and a diagnostic key for Onosma species thriving in Afghanistan are provided and the new species is compared morphologically with its closely related species. In addition, a distribution map is provided for the new species and its close relatives in the area.
Narjes Rezazadeh Moghadam, Asghar Zamani,
Volume 10, Issue 4 (3-2024)
Abstract
The family Verbenaceae, currently consists of Verbena and Phyla genera in different parts of Iran, especially northern provinces. The frame of this family has undergone significant changes in comparison with the previous references. For example, Vitex and Clerodendrum have been defined as the genera of this family in previous references. Accordingly, in this study, the relationship among these four genera has been evaluated using morphological and leaf anatomical characters of 20 samples. For this purpose, 67 qualitative and quantitative morphological and anatomical traits were used. The analysis of data was performed using R software ver. 4.3.1. For simultaneous analysis of quantitative and qualitative data, Factor Analysis of Mixed Data (FAMD) method was applied. The results of this study indicate the high value of some quantitative traits such as main vein xylem length, seed width, style length and blade width and some qualitative traits such as blade epidermal cells shape, stomata position, two arms of blade position in relation to each other, indumentum type, main vein epidermal cells shape, petal color, blade vascular bundles number, density of indumentum, leaf margin shape and stem branches length in the clustering of the genera. Totally, Analysis of data led to the separation of these genera. In accordance with the phylogenetic studies, Vitex and Clerodendrum show more affinity to each other and are separated from the currently native members of Verbenaceae in Iran, i.e. Verbena and Phyla.
Fatemeh Jafari, Ali Ganjeali, Elham Amjadi,
Volume 10, Issue 4 (3-2024)
Abstract
Nepeta binaludensis jamzad, as a medicinal plant, which is at risk of extinction due to irregular harvesting and damage of its habitat. Four treatments including: 1- inoculation with Azotobacter chooroccum, 2- inoculation with Bacillus cereus and Pseudomonas putida, 3- inoculation with a mix of three bacteria (A. chooroccum + B. cereus + P. putida) and 4 controls (without inoculation), in the form of a completely randomized design with 3 replications in two vegetative growth stages (ten and twenty weeks after sowing). Plant growth-promoting bacteria (PGPB) improved all the evaluated morphological characteristics, including the length and dry weight of the aerial part, total leaf area and root length compared to the control. Also, the results of biochemical investigations showed that in 10 and 20 weeks old plants, the application of PGPB could increase the phenolic and flavonoid compounds and also antioxidant activity. The contain of some elements such as calcium, potassium, phosphorus, iron and magnesium also increased as a result of inoculation with PGPB. Since the PGPB improved the growth and also increased the effective compounds of N. binaludensis plants, they can be introduced as useful bio fertilizers and considered as a good alternative to chemical fertilizers.
Dr Fateme Mousavi,
Volume 10, Issue 4 (3-2024)
Abstract
Quinoa seed (Chenopodium quinoa) is considered a unique food source due to its rich protein content and high antioxidant activity related to polyphenols. In the present study, with the aim of selecting the quinoa seeds to send to space, the response of protein content, phenol, flavonoid, antioxidant capacity, and the germination index of its seeds to simulated vacuum conditions of space was evaluated. The results showed a significant increase in the seed germination index for the vacuum-treated group compared to the control group. Total phenolic and flavonoid content was higher in vacuum-treated seeds compared to the control group. Vacuum conditions significantly increased the antioxidant capacity of quinoa seeds. The total seed protein content in the vacuum-treated and control groups was 25 and 35 mg/ml, respectively. The seed protein profile showed 13 distinct protein bands in the molecular weight range of 15 to 70 kilodaltons. The intensity of protein bands was significantly different between vacuum treatment and control groups. Structural changes in the seed pericarp as well as water and oil exit from the seeds under vacuum conditions can be the causes of different biochemical and physiological responses of quinoa seeds in the present study.
Saeideh Khamushi, Fatemeh Nejadhabibvash,
Volume 11, Issue 1 (6-2024)
Abstract
The aim of this study was to evaluate the changes in the essential oil chemical composition of Satureja bachtiarica Bunge were inoculated by spores of the arbuscular mycorrhizal inoculation Glomus caledonium and foliar application of zinc chelate levels (0, 2, 4 and 8 g/L) in 2019, at Zarrin Giah greenhouse of Urmia city in West Azerbaijan Province, in a randomized complete block design with three replications. Plants were harvested at full flowering stage. Essential oils were obtained by Clevenger apparatus and were analyzed by GC and GC/MS. Comparison of means by Duncan's multiple range test showed that inoculation with Glomus caldonium had no significant effect on the essential oil yield of Satureja bachtiarica flowering shoots, but the foliar application of zinc chelate had statistically significant effect. However, there was no difference among different concentrations of zinc chelate in terms of effect on essential oil yield. The treatments also changed the essential oil composition compared to the essential oil of the control group. These changes included the reduction of beta-caryophyllene, sabinen hydrate, borneol and carvacryl acetate compounds and the increase of citral and octyl phthalate in the plant inoculated with mycorrhiza, as well as the increase of carvacrol in the essential oil of plants treated with zinc chelate. Also, inoculation with mycorrhizal fungi and zinc chelate foliar application increased the amount of sesquiterpene & diterpene and monoterpene compounds, respectively.
Mrs Nasrin Mehdinejhad Moghadam, Dr Omid Sofalian, Dr Naser Zare, Dr Mohamad Sedghi, Dr Rahmatollah Karimizadeh, Dr Fatemeh Mohamadiazar,
Volume 11, Issue 1 (6-2024)
Abstract
Legumes are common crops in arid and semi-arid regions. This study was conducted to investigate three levels of salinity stress on 18 lentil cultivars in the greenhouse and laboratories of Mohaghegh Ardabili University in 2016-2016 in a factorial manner in the form of a randomized complete block design with three replications. The results of comparing average lentil genotypes were done with Duncan's method at the five percent probability level. Correlation was done in separate stress levels. The dendrogram of cluster analysis divided the genotypes into three groups in control and 60 mM conditions and two in 120 mM conditions. Genotypes 7 and 5 were in the top group in all groupings. The relationship between ISSR molecular markers and physiological traits was calculated among genotypes. All traits in three levels of salinity stress had a significant correlation with some ISSR markers. A total of 22 positive markers for physiological traits were identified at the control level, 9 positive markers at the moderate stress level, and 23 positive markers at the severe stress level. Among the 21 studied ISSR primers, the P8A11 marker had the largest number of gene loci related to the studied physiological traits.
