Showing 33 results for Plant
Kobra Gholipoor, Parto Roshandel,
Volume 5, Issue 4 (12-2018)
Abstract
In order to study the diverse effects of seed priming with 24-epibrassinolide (EBL) (0, 0.1, 1 mg/l) to increase tolerance of tomato plants (Lycopersicon esculentum Mill.) to salinity (0, 70 and 140 mM NaCl), the experiments were conducted as factorial based on completely randomized design at greenhouse condition. Results showed that salt stress significantly decreased the growth of tomato plants. However, priming with EBL (1 mg/l) significantly decreased the negative effects of NaCl (particularly at 140 mM). At this case, EBL increased the fresh and dry weights (<2 folds), total chlorophyll (+72%), carotenoids (+ 2 folds), free proline (+3 folds), electrolyte leakage (-18.6%), Na+/K+ in the roots (-57.5%) and shoots (-62%) compared to salinity control. Generally, it could be concluded that priming with epibrassinolide resulted in enhanced salt tolerance in tomato plants via increment in free proline, photosynthetic pigments and decrease in electrolyte leakage and Na+/K+ ratio in the tissues.
Maneezheh Pakravan, Sahar Moradpoor, Azizollah Jafari,
Volume 5, Issue 4 (12-2018)
Abstract
Vegetation is one of the most important parts of natural ecosystems that in addition to express of the growth conditions, it also shows the influence of ecological factors on the environmental condition. Investigations of plant diversity of a country are necessary for studyingthe ecosystem, pasture, plant gene bank, agriculture and medicine. On the one hand, identification of plant species of different regions provide feasibility of doing of next studies in the different fields of biological science. In this research the flora of Tang sorkh region in the Kohgiloyeh county from Kohgiloyeh-va boyerahmad province has been investigated. The area of mentioned region is 5000 hectares and it is located in 30 kilometers from the east of Yasuj. Maximum altitude of the area was between 1800-2800 m. Our research has started by collecting the essential information of the region. The plants of region have been collected and prepared for studying. several floras were used for identification the specimens and they are preserved in Alzahra University herbarium (ALUH). This investigation showed that in the Tang sorkh region, there are 46 families, 145genera, 172 species that include 6/97% of phanerophytes, 5/23% chamephyts, 43/60%hemicryptophytes, 13/95% geophytes, 28/48% therophytes. Analyses shows that most of species of region belongs to the Iran-o-Turanian region which contanins 5/69% of region’s flora. 23 species are endemic to Iran, 20 of which are rare species, 30 species are medicinal plant, 22 of which are poison. Totaly about 172 species has identified. 20 species are recorded for the first time from this province.
Katayoon Meimandi, Mohammad Mehdi Yaghoobi,
Volume 6, Issue 1 (5-2019)
Abstract
In this study, the cytotoxic effects of aqueous and ethanolic extracts of Sedum album L. on human stomach cancer cell line (AGS) and breast cancer cell line (MCF-7) were evaluated by MTT, BrdU and TUNEL assays. The results demonstrated that both extracts had antiproliferative and apoptotic effects in a dose-dependent manner. The MTT assay data revealed that the AGS cell underwent more cytotoxicity in comparison with the MCF-7 cell. It also revealed that ethanolic extract was more potent than aqueous extract. The BrdU assay results showed that the proliferation of AGS and MCF-7 cells was reduced to 50% and 43%, respectively, at the highest concentration of the aqueous extract. In addition, the ethanolic extract reduced the proliferation of AGS and MCF-7 cells to 75% and 60%, respectively. The AGS and MCF-7 cells underwent 52% and 12% apoptotic death upon treatment by the ethanolic extract as TUNEL assay showed. The aqueous extract induced 28% and 25% apoptosis in the AGS and MCF-7 cells, respectively. Both inhibition of proliferation and induction of apoptosis are desirable strategies for cancer treatment among researchers. Identification of S. album compounds and analyzing their effects in animal model of cancer can help us with understanding its anti-cancer properties.
Maryam Khezri,
Volume 6, Issue 1 (5-2019)
Abstract
Bacterial communities are able to form complex and three-dimensional biofilm structures. Biofilm formation is an ancient and integral component of the prokaryotic life cycle and a key factor for survival in diverse niches. In biofilms, bacterial lifestyle changes from free-floating cells to sessile cells. Presence in biofilms gives new traits to bacteria, which distinguish them from free cells. The presence of bacteria in biofilms results in high resistance to antimicrobial treatments and oxygen deficiency. Biofilms are formed in response to different environmental signals and many genes are involved in their production. Biofilms can be problematic in fluid transfer pipelines, on medical devices, as well as implants in the patients’ bodies. However, they can be applied for useful purposes such as treating industrial and agricultural wastewater, bioremediation of heavy metals and in air pollution biofilter systems. The potential of forming biofilms in pathogenic bacteria is an advantage for their survival in unfavorable conditions, and cause a lot of problems in their removal as the bacteria show more resistant to antibiotics and chemical pesticides in biofilms compared with free living cells. The ability to form biofilms in plant-beneficial rhizobacteria used for plant disease biocontrol, plant growth promotion and the improvement of agricultural products quality is an important advantage especially in their mass production and commercializing process. Considering the importance of bacterial biofilms in human life, this paper evaluated the importance of biofilms from different aspects.
Seyed Mousa Mousavi Kouhi, Maryam Moudi, Esmael Soltani Moghadam, Hedyieh Sarchahi Moghadam,
Volume 6, Issue 1 (5-2019)
Abstract
The identification of the characteristics of native halophytic species is very important for their practical application. The present study was carried out to identify the halophytic species of a desert and highly saline region around the city of Khosf, southern Khorasan Province, and to detect their general tolerance mechanisms (i.e. salt exclusion or inclusion). Sodium accumulation in the roots and shoots of seven species, including Launaea arborescens, Peganum harmala, Pteropyrum olivieri, Artemisia santolina, Zygophyllum eurypterum Boiss, Aerva javanica, Pulicaria gnaphalode, and their rhizosphere soil were determined. The bioconcentration of sodium from soil to roots (BCF), its translocation from roots to shoots (TF), and its accumulation in the shoots (AF) were then calculated. Results showed that the soil of the studied area was clay loam with a high EC of 65 ds/m, indicating its high salinity level. According to the values of sodium adsorption ratio (SAR) and the exchangeable sodium percentage (ESP) and regarding EC, the soil of studied region can be regarded as a saline-sodic soil. The value of TF in some species was higher than 1. However, none of the species had BCF and AF greater than 1, thus, none of them could be considered to be salt accumulator. Instead, it could be assumed that all of the studied species were salt, or at least, sodium excluders.
J-Avad Baharara, Tayebe Ramezani, Negar Saghiri, Farzane Salek,
Volume 6, Issue 2 (8-2019)
Abstract
In recent years using of silver nanoparticles due to its unique properties was increased. Medical plant, the Achillea biebersteinii is rich in anti-cancers compounds. The aim of this study was to investigate the cytotoxicity effects of AgNPs synthesized using Achillea biebersteinii extract on human ovarian cancer A2780 cells. Cytotoxic effects of AgNPs with MTT test was performed at 48 hours in concentration of 2, 4, 6, 8, 16 and 32 µg/ml. To study the cell death induced by AgNPs DAPI, acridine orange (AO)/ Propidium iodide (PI) staining and Annexin v/ Propidium iodide assay and activation of caspase 3 and 9 were assessed. Results: The results showed that the synthesized AgNPs decreased cell viability dose dependently, calculated inhibitory concentration of 50 % (IC50) was 4 µg/ml. The results from AO / PI, DAPI staining, Annexin V / PI showed that in treated cells the percentage of apoptotic cells compared with control increased. In addition, the treated cells showed increased activation of caspase 3/9. Hence silver nanoparticles induced cell death through the caspase dependent pathway. The results suggest that AgNPs synthesized using Achillea biebersteinii extract exert their cytotoxic effect by inducing apoptosis.
Fereshteh Heidargholinejad, Hossein Moradi, Mahnaz Karimi, Vahid Akbarpour,
Volume 6, Issue 2 (8-2019)
Abstract
Purslane (Portulaca oleracea L.) contains valuable secondary metabolites such as Dopamin, Noradrenaline and Omega-3. This plant is used in various medicinal, food and hygienic industries as well as the treatment of different diseases such as diabetes, heart disease and pain relief. Callus induced from medicinal plants are used to increase the production of secondary metabolities in cell suspension culture and gene transfer. The purpose of this experiment was the study of different concentrations of BAP and 2,4-D of two explants from leaf and shoot tips to produce callus. Leaf and shoot tip explants were used in MS with different concentrations of BAP at three levels (0, 1 and 2 mg/L) with 2,4-D at three levels (0, 0.5 and 1.5 mg/L). Results showed that interactions between hormones and explants were significant in the percentage of callus induction, fresh weight and callus diameter at 1% level. The best result which was the leaf explant with 100% callus induction, 121 mg fresh weight and 5.106 mm callus diameter was obtained by the combination of BAP 2 mg/L and 2,4-D 0.5 mg/L. Shoot tip explants with 75% callus induction, 106 mg fresh weight and 3.03 mm diameter was obtained by the application of 1 mg/L BAP and 0.5 mg/L 2,4-D.
Seyed Reza Safavi,
Volume 6, Issue 3 (10-2019)
Abstract
Scorzonera incisa, known as an endemic plant to Turkey, has been collected from West Azarbayejan Province, NW. Iran. The species is reported for the first time from the country; it is very similar to Scorzonera calyculata, which is distributed in the western half of Iran and east of Iraq. However, with regard to the particular shape of the achenes of Scorzonera incisa, this species could be separated from Scorzonera incisa. In this paper, the description, the image of the herbarium sample and the distribution map of this species are presented.
Fatemeh Derikvand, Eidi Bazgir, Mostafa Darvishnia, Hossein Mirzaei Najjafgholi,
Volume 6, Issue 4 (12-2019)
Abstract
Antioxidant enzymes play an important role in plant defense against pathogenic agents. Following the identification of the pathogen, plants produce active oxygen species (ROS) as one of their first defense responses. To maintain the balance of ROS levels and prevent their harmful effects, plants produce antioxidant peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX) and superoxide dismutase (SOD) enzymes. In the present study, the resistance of bean plants cultivars, namely Sadri, Paak, Darakhshan and Dorsa, to Xanthomonas axonopodis pv. phaseoli (Xap) were studied in greenhouse conditions. The catalase, peroxidase and ascorbate peroxidase enzyme activities were studied in healthy and Xap-infected bean cultivars Sadri and Derakhshan at 0, 24, 48, 72 hours and 20 days post inoculation by a completely randomized design with 5 treatments and 4 replications. The result showed that disease symptoms appeared in all tested cultivars. Derakhshan and Sadri cultivars, with 58.33 and 80.56 percentages of infected plants 20 days after inoculation, showed the least and highest infection rates, respectively. The highest catalase and peroxidase activities were recorded 24 and 48h post inoculation. These records reduced 48 and 72 hours post inoculation, respectively. The activities of these two enzymes in the susceptible cultivar were less than those in the semi-resistant one. The chlorophyll a and chlorophyll b contents of Xap-infected plants reduced significantly. The total chlorophyll content of uninfected Sadri and Darakhshan cultivars were 2.93 and 3.23 µg/g, respectively, which reduced to 1.96 and 2.14 µg/g of leaf tissue in infected plants, respectively. Based on these results, it is suggested that the Derakhshan cultivar should be planted in disease-susceptible regions as the semi-resistant cultivar.
Mehrnoush Daneshvar, Mahmood Maleki, Shahryar Shakeri, Amin Baghizadeh,
Volume 6, Issue 4 (12-2019)
Abstract
Phosphorus, the most essential nutrient for plants, becomes quickly unavailable for the plants in the soil. Phosphate solubilizing bacteria (PSB( can play an important role in providing Phosphorus for plants. In this study, the PSBs were screened from plant rhizosphere by Pikovskaya method. Then, the growth rate and phosphate solubilizing ability of 9 superior strains were measured at different temperatures and levels of salinity and pH. The best strain was identified by 16S rDNA gene sequence analysis. Finally, the genetic diversity of phosphate solubilizing strains were examined by RAPD markers. Results showed that 25 strains were capable of solubilizing insoluble phosphates among the 57 isolates studied. Of the nine superior strains, Cke1 had the highest solubilizing index with the average growth rate under all conditions and was introduced as the best PSB strain identified in the present study. 16S rDNA gene sequence analysis showed that this strain belonged to the Enterobacter genus. The results of genetic variation showed that all stains were divided into six groups and three strains that had the lowest similarity with other strains were placed in three separate groups. Given that Cke1 strain has the ability of solubilizing the insoluble phosphate in different stresses, it can be a good candidate for providing phosphorus at temperatures of 30 and 35 °C, 1.2% and 1.8% salinity levels and pH levels of 6 and 8 for the crops.
Maryam Masoudizadeh, Parzhak Zoufan, Saadat Rastegarzadeh,
Volume 6, Issue 4 (12-2019)
Abstract
The cement production activities leads to the release of different pollutants into the environment. This research was conducted to study the effects of dust particles released by Behbahan cement factory on the absorption of some nutrient elements and biochemical parameters in dominant herbaceous species grown in this area, including Bromus tectorum, Malva neglecta and Sinapis arvensis. Plant samples were transferred to the laboratory. Then, the concentrations of some nutrient elements, such as Fe, Cu, Zn, K, Mn, P and N, were assayed in plant and soil samples. Moreover, some biochemical parameters, such as photosynthetic pigments, total protein, soluble carbohydrates, proline, relative water content, leaf pH total ascorbate and air pollution tolerance indexes (APTI), were evaluated. Based on these results, it is supposed that three plant species are tolerant to air pollution with an APTI higher than 16. In addition, it seems that the dust released by the cement factory has not led to a toxic accumulation of the elements in the studied plants. The deficiency of Mn and P was determined for three plant species. On the basis of biochemical analysis, it is supposed that these plant species possibly use different strategies to tolerate the pollutants in this area.
َamjad Saedi, Hossein Moradi, Mahnaz Karimi,
Volume 6, Issue 4 (12-2019)
Abstract
Aloe vera L. is one of the most valuable plants in the pharmaceutical, cosmetic, sanitary and food industries. In vitro culture is used for commercial production and due to the abundant application of this plant, extensive research has been performed on the in vitro culture of Aloe vera. For this purpose, the present study was conducted at two stages. At the first stage, the best method of sterilization of explants derived from Aloe vera offshoots was investigated. At the second stage, the effect of the type of explants, the light condition (dark and bright) and the effect of BAP (Benzyl Amino Purine) and NAA (α-Naphthalene acetic acid)) growth regulators on regeneration and the amount of phenolic compounds were studied. A factorial experiment was executed on the basis of a completely randomized design with three replications. The best sterilization protocol was 0.1% mercuric chloride (for 2 minutes), 70% ethanol (for 30 seconds) and 15% sodium hypochlorite (for 5 minutes). The little white explant derived from the base of leaves, with the lowest percentage of phenol and the highest survival rate (67.5%) in darkness, was found to be the best candidate. MS medium supplemented with 0.75 mg / L BAP and 0.25 mg / L NAA resulted in the highest stem number (2.5) and stem length (42.107 mm), establishment percentage (73%), leaf number (6.33), leaf diameter (4.8 mm), chlorophyll b (9.216 mg/g) and carotenoids (4.81 mg/g). The highest content of chlorophyll a (56.07 mg/g) and total chlorophyll (61.35 mg/g) were found in samples treated with hormonal medium, supplemented with 1.5 mg / L of BAP with 0.5 mg / L of NAA. The maximum number (3) and average length (33.3 mm) of roots were observed in samples treated with the hormone-free medium.
Mehrdad Ghiasvand, Ali Makhdoumi, Maryam Moghaddam Matin, Jamil Vaezi,
Volume 8, Issue 2 (7-2021)
Abstract
Regarding the rising rate of esophageal cancer in some parts of Asia, including northern and northeastern regions of Iran, the identification of new anti-cancer compounds is essential to combat the disease. The relation between some plants metabolites and these endophytic microorganisms are well characterized. In the current study, the potentials of Ephedra endophyte bacteria for the inhibition of esophageal cancer cell line (KYSE-30) proliferation were investigated. A total of 54 endophyte bacteria (out of 70) were obtained from the sterilized surfaces of two medicinal plants, i.e., Ephedra intermedia and Ephedra foliata. Bacterial strains were then cultured in Tryptic soy broth (TSB) medium and, after 72 h incubation, the produced secondary metabolites were extracted by chloroform. Anticancer effects of secondary metabolites from theses bacteria on esophageal cancer cell line KYSE-30 were evaluated after 24, 48 and 72 h by MTT method. MTT assay results showed that only strain A1 had a cytotoxic effect on KYSE-30 cells. The IC50 amounts of this strain against KYSE-30 cell lines were equaled (µg/ml) to 346.4, 192.8 and 121.3 after 24, 48, and 72 hours, respectively. The molecular identification of strain A1 revealed that Microbacterium maritypicum (99.8% similarity) was the closest identified taxon to the strain studied. According to the promising ability of strain A1 to inhibit the growth of KYSE-30 cell line, the use of natural compounds produced by this bacterium to treat esophageal cancer was found to be applicable. However, more experiments are needed to confirm these results after purifying the ingredients, as well as conducting studies in animal cancer models.
Alireza Amini Hajiabadi, Asghar Mosleh Arani, Someh Ghasemi, Mohammad Hadi Rad, Shima Shabazi, Hassan Etesami,
Volume 8, Issue 2 (7-2021)
Abstract
Salinity stress is an important challenge for wheat production in the world. Plant growth promoting rhizosphere bacteria, isolated from halophytic plants, can increase the tolerance of crop plants to salinity by direct and indirect mechanisms. In this study, plant growth-promoting traits of bacterial strains (Bacillus safensis, Bacillus pumilus and Zhihengliuella halotolerans), isolated from the rhizosphere of several halophyte plants, were deterimined and their effects on some vegetative traits and ionic content of wheat plant irrigated with saline water ( 0.2, as control, 4, 8 and 16 dS/m) were measured. Result showed that all three bacteria were able to produce auxin, hydrogen cyanide, siderophore, 1-aminocyclopropane-1-carboxylic acid deaminase and soluble phosphate. The increase in salinity levels caused increase in the concentration of sodium and decrease in the concentration of potassium, calcium and phosphorus in wheat leaves, as well as decrease in stem length, shoot and root dry weight, root to shoot dry weight ratio and total biomass. In wheat plants irrigated with saline water and inoculated with the bacterial strains, sodium concentration decreased up to 17.7% and concentrations of potassium, calcium, phosphorus and potassium to sodium ratio increased up to 33, 25.7, 200.4 and 41%, respectively. The most efficient bacterium was found to be Z. halotolerans. All bacterial isolates also increased stem length, shoot and root dry weight, root to shoot dry weight ratio and total biomass by 17, 58.6, 137, 88 and 66 %, respectively. The results of this study showed that the plant growth-promoting bacteria of rangeland halophytic plants potentionally improve the growth indices of wheat plants in saline conditions. These results also showed that the rhizosphere of halophytic plants in rangelands can be a good source for the isolation of salinity-resistant bacteria to improve the resistance of wheat plants to salinity.
Asrin Arshi, Dr Azam Salimi, Maryam Chavoushi,
Volume 8, Issue 3 (10-2021)
Abstract
Tomato (Solanum lycopersicum) is one of the most important crops and a known host of broomrapes. The species of genus Orobanche are biotic stress factors restricting plant growth and crop production. Some natural compounds and chemicals affect this biological relationship. Therefore, in the present study, the effect of gallic acid and kiwi fruit extract on the biological relationship between broomrape and tomato plant was investigated. To this end, first, the symbiotic effect of the broomrape species, Orobanche aegytica, on growth parameters of tomato plant and the amount of soluble sugar and photosynthetic pigments were evaluated. Then, the effect of concentrations of gallic acid and kiwi fruit extract at different levels on this biological relationship was investigated by measuring the growth parameters of tomato and broomrape, photosynthetic pigments and the amount of soluble sugar in tomatoes. The results demonstrated that the broomrapes reduced the fresh weight and dry weight of the stem, leaves and roots of tomato, diminished the leaf area of the host plant, and decreased the amount of photosynthetic pigments and its soluble sugar content. Gallic acid treatment and kiwi fruit extract lessened the growth parameters of the broomrape species and its overall growth, in addition to increasing the growth of tomato plants. This process could be associated with the constraint of the biological relationship between the parasite and the host, as the soluble sugar content as well as the amount of the photosynthetic pigments of tomato plants were increased and the tomato plants remained highly resistant.
Mahdis Meraji Masouleh Moghaddam, Farhad Mashayekhi, Ziba Zahiri, Akram Eidi,
Volume 8, Issue 4 (12-2021)
Abstract
This study aimed to investigate the polymorphism of matrix metalloproteinase -3 (MMP-3) gene and its expression in the serum of infertile female patients received in vitro fertilization and embryo transfer (IVF-ET). To do so, 100 women with unsuccessful IVF-ET (IVF–) and 100 women with successful IVF-ET procedure and clinical pregnancy (IVF+) were included. Genetic polymorphism and serum concentration of MMP3 were investigated by ARMS-PCR and ELISA, respectively. The results showed no significant association between MMP-3 gene polymorphism and IVF-ET outcome among the two groups studied. However, a significant decrease in the concentration of MMP-3 serum in the IVF– group was observed in comparison with the IVF+ group (P=0.000002). Moreover, we showed that the serum MMP-3 levels in CC, AC and AA genotypes in the IVF– group were 33, 65.33 and 86 ng/ml, respectively. In conclusion, while there is no significant difference between MMP-3 promoter polymorphism and IVF-ET outcome between the IVF+ and IVF- groups, a significant decrease in MMP-3 serum levels in IVF- group was seen as compared with the IVF+ group. It could be also suggested that the CC genotype is associated with a decreased level of MMP-3 serum concentration and may be associated with IVF-ET failure.
Raheleh Ahmadpour, Roya Ghalavand, Saeed Reza Hosseinzadeh, Nezam Armand,
Volume 9, Issue 4 (12-2022)
Abstract
Successful germination is the main factor in proper establishment and seedling yield. Information on seed germination, in the face of temperature changes at the time of planting, is a way to identify more suitable cultivars for cultivation at the planting site. For this purpose, a factorial experiment was performed based on completely randomized design with 3 replications. This experiment was conducted to investigate the temperature changes on the germination and growth indicators of lentil cultivars at Khatam Al-Anbia University of Behbahan. The studied treatments included five lentil cultivars of Gachsaran, Kimia, Robat, Ziba and Bilehsevar, and the temperature levels were 20, 25 and 30 °C, similar to the autumn conditions of Khuzestan Province. The results showed that all the seedlings of investigated cultivars cultivated at temperatures of 20 and 30 °C had a significant decrease in all the investigated traits, i.e., germination percent, germination rate, germination vigor, seed vigor index, endosperm consumption, plumule length, radicle length, seedling length, plumule dry weight and radicle dry weight, in comparison with those seedlings cultivated in 25 °C. The study of the interaction of cultivar-temperature effects showed that Bilehsevar cultivar had the highest germination and growth indices at 25°C compared to other investigated cultivars. This increase in traits such as germination rate, germination vigor, seed vigor index, plumule dry weight and radicle length was more significant in Bilehsevar cultivar compared to other cultivars. The second rank of the highest germination and growth indices was assigned to Gachsaran cultivar under 25°C conditions, which had a significant increase compared to other cultivars (Kimia, Robat and Ziba). Considering the autumn cultivation of lentils and the changes of temperature in that season (temperature range of 20 to 30 °C) in the studied area, the use of Bilehsevar and Gachsaran cultivars under the temperature of 25 °C, due to their higher germination indicators compared to the other cultivars, i.e., Ziba, Kimia and Robat, could be recommended.
Azadeh Niknejad, Alireza Shafizadeh Esfandabadi, Fatemeh Abdollahi Sarvestani,
Volume 10, Issue 1 (6-2023)
Abstract
The emergence of new viruses has always been a threat to the health of people around the world, the latest example of it is the new strains of the coronavirus (SARS-CoV-2) and the resulting acute respiratory distress syndrome (ARDS). The current situation underscores the importance of rapidly producing low-cost stable vaccines that do not require refrigeration equipment for storage and transportation. However, most vaccines are not yet available in developing countries due to import costs and storage and transportation needs. Therefore, the vaccine must be affordable for developing countries so that vaccination can be carried out on a large scale. Herbal vaccines are more cost-effective than other types of vaccines and production methods and can be produced in large quantities. In addition, herbal vaccines have other benefits that are discussed in this article. However, given that an herbal medicinal product is to be used as a vaccine in a semi-processed form (such as mashed potatoes or tomato paste), specific regulatory reviews must apply to injectable vaccines. Products should also be applied to evaluate their side effects clinically. The current review article investigates the opportunities and challenges of producing plant-based vaccines to deal with diseases like Coronavirus disease (COVID-19(.
Azarnoosh Jafari, Ameneh Assadi Barbariha, Fereshteh Ghasemzadeh,
Volume 10, Issue 1 (6-2023)
Abstract
The present research, a floristic study was executed on Imamverdy, Dartum, Ghapagh, Garivan and Niestaneh villages, located 30 km south of Bojnourd (North Khorassan province) with altitudinal range of 1442-1783 m. The present study aimed to identify the specimens, their possible medicinal properties, endemism and concervation status. For this purpose, the specimens were collected during March-November 2012 and identified using Flora of Iran and Flora Iranica. The results showed the presence of 133 species from 104 genera and 38 families, one species belonged to Gymnosperms and the rest, 132 species, belonged to Angiosperms, of which five families, 13 genera and 18 species were monocotyledons and 32 families, 90 genera and 114 species were dicotyledons. Moreover, 40 species were found to have medicinal applications in the studied region. Fabaceae, Brassicaceae, Poaceae, Asteraceae and Lamiaceae were the largest families and Astragalus, Poa, Vicia and Euphorbia were reported as the largest genera in the region. Erysimum koelzii, Astragalus khoshjailensis, Eryngium bungei, Acanthophyllum pachystegium, Sclerorachis platyrachis, Taraxacum hydrophyllum were endemic species for Iran. Astragalus ackerbergensis was found to be in vulnerable status and Rubia florida, Mentha longifolia, Fumaria vaillanti and Onopordum carmanicum were found to be at low-risk status. The maximum and minimum percentages of chorotypes were related to Irano-Turanian with 68.14% and Irano-Turanian, Saharo-Arabian regions with 0.7%, respectively. The maximum and minimum percentage of life form were reported to be hemicryptophytes with 45.8% and cryptophytes with 3%, respectively. The results reflected the cold and arid climate and uncontrolled grazing in the studied region.
Ali Ganjeali, Reyhaneh Bahrami Totmaj, Parwaneh Abrishamchi,
Volume 11, Issue 3 (12-2024)
Abstract
The present study aimed to investigate the effect of some bio-elicitors and silver nitrate on the content of secondary metabolites in calli derived from leaf explants of silybum marianum. In this research, the best callus was achieved on MS medium containing KIN, thus this medium was chosen for further experiment. In the second experiment, leaf explants were cultured in a selected hormone-regulated medium. After 8 weeks of callus growth, bio elicitors including yeast Yarrowia lipolytica, fungus Aspergillus niger, and bacterium Pseudomonas putida in combination with silver nitrate as a non-living elicitor were added to the leaf-derived calli. Ten days after treatment, the phenolic compound content, antioxidant capacity, silymarin content, and PAL enzyme activity in the calli were measured. The results indicate that the simultaneous application of Yarrowia yeast and silver nitrate led to an improvement in the content of phenolic derivatives, silymarin, and PAL enzyme activity, and this was significant compared to other elicitors both with silver nitrate and without it. The researchers of this experiment suggest that the combined use of bio-elicitors especially Yarrowia lipolytica yeast, and silver nitrateinduces higher synthesis of medicinal metabolites in calli derived from leaf explants.
