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Showing 8 results for Nasri

Nasrin Parvaresh, Mojtaba Mortazavi, Masoud Torkzadeh-Mahani,
Volume 4, Issue 1 (6-2017)
Abstract

Cutinase belongs to the family of serine hydrolases which are capable of hydrolysis of esters and small polyester such as cutin. The cutin of plants is composed of hydroxy and epoxy fatty acids and is substantially imperm-eable to water and only the plant pathogenic bacteria are capable of degrading it. Initially, in order to identify the extent of cutin in cucumber (Cucumis sativus' C. sativus'), golden apples (Golden Delicious apple 'Malus domestica'), red ap-ples (Red Delicious apple 'Malus domestica'') and tomatoes (Solanum lycopersicum Mill. Commun ' S. lycopersicum'), their fruits were obtained. Cutin was extracted from the fruit skin of each studied specimen and compared by chloro-form-methanol method. Then, isolation of enzyme-producing strains was performed by the use of the specific medium containing cutin and enzyme activity assay. DNA of the specimens was extracted, and PCR were performed with univ-ersal primers for 16s DNA. The remainder was devoted to bioinformatic analysis, identification and registration of samples in the gene bank. The results showed that the percentage of extracted cutin in red apple was more than other samples and as a result, red apple are expected to be more resistant against diseases and pests. Also, six cutinase-prod-ucer strains of Klebsiella and Enterobacter were isolated with the help of enzyme activity and special culture medium containing cutin, and their 16s DNA region coding sequences were recorded in the GenBank.


Nasrin Nikmehr, Soheil Eagderi, Pariya Jalili,
Volume 4, Issue 1 (6-2017)
Abstract

Kura bleak, Alburnus filippii, a member of the family Cyprinidae, is found in the rivers of Aras and Sefidrud, and the Anzali lagoon of Iran. The current study was conducted to provide a detailed osteological description of this sp-ecies since there was not adequate information in this regard. In order to attain this goal, 20 specimens were collected from the Ahar Chay River by electrofishing device, and fixed in 10% buffered formalin. The specimens were cleared, stained with alizarin red S and alcian blue for osteological examination and a detailed description of its skeletal structure was provided. The results showed that this species can be distinguished from other members of this genus in Iran by a combination of osteological characters, including having pointed process of the maxillae, dorsal tilting of the coronoid process, protruding of the anterior margin of the hyomandibular, fan-shaped anterior part of the basihyal, pointed end of the pre-vomer posteriorly, small fourth infraorbital and 11 pterygiophors in the dorsal fin.


Mohsen Fatemi, Nasrin Mollania, Madjid Momeni-Moghaddam, Fatemeh Sadeghifar,
Volume 6, Issue 1 (5-2019)
Abstract

New properties of nano-materials have made nanotechnology the leading part of biology and medical sciences. Due to their various biomedical properties, iron-based magnetic nanoparticles (MNPs) have been highly considered by biological researchers. Nowadays, increasing resistance to antibiotics is a major problem in treating clinical infections. Finding new antibacterial agents is therefore essential for the treatment of resistant strains. In this study, the iron oxide MNPs were produced using culture-medium supernatant of a newly isolated bacterium to investigate the inhibitory effects of the NPs on strains with a major role in clinical infections. Biosynthesis of iron oxide MNPs were detected by UV-Vis spectroscopy and the average size of particles was estimated by dynamic light scattering technique. The anti-bacterial activity of these NPs against E. coli and S. aureus was investigated using methods for the calculation of bacterial sensitivity coefficient. In the presence of NPs, the highest sensitivity coefficient value was observed for E. coli in 1xMIC concentration. On the other hand, S. aureus showed the lowest value. The death rate of the two strains in contact with NPs followed the first order kinetic equation and the survival rate decreased with the increase of exposure time. The results of this study as well as the high functionality of iron oxide MNPs, make its application desirable in the prevention and treatment of clinical infections.

 


Mojtaba Mortazavi, Nasrin Parvaresh, Masoud Torkzadeh,
Volume 6, Issue 1 (5-2019)
Abstract

Cutin is a polymer that is constructed in plants by the condensation and oxidation of fatty acids and plays a key role in the protection of plants against pathogens. Cutinase is a hydrolase enzyme that breaks down the cutin. The purpose of this study was to extract cutin from red apples with oxalate buffer, cutinase enzyme activity assay in LB culture, and bioinformatic analysis. To attain these purposes the cutinase-producing strains that had previously been isolated were inoculated in culture medium containing cutin. After initial culture, the bacteria were cultured in LB medium and cutinase activity was measured using the p-Nitrophenyl butyrate. In order to execute bioinformatic analysis, the isolated sequences of six cutinase-producing bacteria were analyzed based on computational data bases and their phylogenetic trees were prepared. Then, the similarities in the sequences of a large number of cutinase-producing samples were analyzed by drawing the phylogenetic tree. The results showed the separation of cutinase-producing prokaryotes from cutinase-producing eukaryotes. Then, the sequence of 16S rDNA of these cutinase-producing samples as well as the samples we had prepared were evaluated and their phylogenetic relationships were determined. This analysis showed that the new sequence stood alongside the bacterial samples. Thus, our cutinases may be similar with these bacterial cutinases in structure and function.
 

 
 


Manoochehr Nasri, Fariborz Ghojoghi,
Volume 6, Issue 4 (1-2020)
Abstract

In order to investigate the differences in the shape of the big-head and two morphotypes of Phytophagous fish by geometric morphometric technique, 30 samples of each group, with a mean standard length of 25 ± 3 cm, were prepared. After fish were anesthetized by 1% Clove powder extract and stabilized in formalin buffer 4%, 6-megapixel digital images of the left profile of the specimens were prepared. A total of 17 morphological landmarks were digitized by tpsDig2 software and the tps files were prepared. All non-shaped data were removed from landmark coordinates by generalized procrustes analysis. After the approval of regression between shape space and tangent space, data were analyzed using Principal Component (PCA), Canonical Variation (CVA) and Cluster Analyzes (CA) methods in PAST and TPS series. According to the results, the head length and height, the size of dorsal fin, the length of pre ventral distance, the height of caudal peduncle and the degree of posterior position of the pectoral fin in bighead carp, are some of the distinctive morphological features among species, mainly related to their ecological niche (specially feeding strategies). In the case of silver carp variants, the posterior position of pectoral fin base in silver carp-R that is close to bighead carp, may be due to hybridization between bighead carp and silver carps.
 


Nasrin Esmailnejad, Jalil Khara, Mehrdad Akhgari,
Volume 6, Issue 4 (1-2020)
Abstract


To investigate the effect of herbicide Trifluralin and the role of magnetized water on enzymatic activities and content of proline and growth substances including gibberellin (GA3) and cytokinin (6- benzyl amino purine), an experiment was conducted using squash (Cucurbita pepo L. var. Shiraz Hybrid F1) seedlings. Four levels of trifluralin (0, 5, 15 and 25 ppm) and irrigation by distilled and magnetized water were applied in a completely randomized design in 3 replicates at Urmia University in 2016. Different levels of trifluralin were added to pots 2 days after planting. Seedlings were treated by modified Hoagland solution with diurnal temperature 30:18 °C, relative humidity of 70-80 percent and light period of 16:8 hr, during a 5-week growing period in growth chamber. The effect of magnetized water and trifluralin on ornithine amino-transferase (OAT), proline dehydrogenase (PDH) and content of proline in both shoots and roots as well as GA3 and cytokinin was significant (p>0.01) according to ANOVA. The content of proline and OAT in roots and shoots as well as GA3 and cytokinin increased significantly, although the PDH of roots and shoots decreased under the influence of magnetized water. The content of proline and OAT increased and GA3, cytokinin and the activity of PDH declined by the increase of the levels of trifluralin. Overall, it can be concluded that the growth and biochemical indices of squash plants were improved by magnetized water under toxicity of herbicide trifluralin.
 

Atta Mouludi-Saleh, Soheil Eagderi, Shima Latif-Nejad, Manoochehr Nasri,
Volume 7, Issue 2 (7-2020)
Abstract

This study was conducted to understand the morphological variation of four populations of Schizothorax pelzami from Iranian inland waters. For this purpose, a total of 81 specimens were collected from the Bidvaz, Cheshmeh-Ali, Kalat and Aal rivers. After anesthesia, all specimens were fixed into buffered formalin and transferred to the laboratory. Then, the left sides of all individuals were photographed. To extract the body shape data, 14 landmarks-points were defined and digitized on 2D image using TpsDig2 software. To eliminate size, direction and position from data, the Generalized Procrustes Analysis (GPA) was performed. Then, PCA, MANOVA/CVA and cluster analysis were used for further investigation. The results showed that there was no significant difference but some overlapping among the studied populations on the basis of PCA analysis was observed. The CVA graph separated the studied populations from on another. The main differences in body shape of the studied populations were related to the position of the snout, the depth of head and body, and the length of caudal peduncle. The observed changes can be considered to be an adaptation to their habitat, which has been influenced by different environmental parameters.
 
 
Atta Mouludi-Saleh, Dr Soheil Eagderi, Keyvan Abbasi, Manochehr Nasri,
Volume 8, Issue 4 (1-2022)
Abstract


 
Atta Mouludi-Saleh1, Soheil Eagderi1, Keyvan Abbasi2 & Manoochehr Nasri3
1Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran; 2Inland Waters Aquaculture Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization, Bandar Anzali, Iran; 3Department of Fisheries Science and Engineering, Faculty of Agriculture and Natural Resources, Lorestan University, Khorramabad, Iran
Correspondent author: Soheil Eagderi, soheil.eagderi@ut.ac.ir
 
Abstract: Due to the presence of taxonomic ambiguity regarding Petroleuciscus ulanus and Alburnus atropatenae and the tendency to treat them as synonyms of each other, this study was conducted to validate these two species by comparing their morphometric and meristic characters in the Mahabad-Chai River, Urmia Lake basin. A total of 64 specimens of P. ulanus and 68 specimens of A. atropatenae were sampled and were then investigated for 17 morphometric and 7 meristic characters. Measurements were standardized to eliminate the effects of size due to allometric growth pattern, then their normality and abnormality were examined. Standardized data were analyzed using t-test, Mann-Whitney, principal component analysis (PCA) and discriminative function analysis based on P-value of Hotelling's t-test (DFA/Hotelling's T-test) methods. The results showed that the two species were significantly different in 11 morphometric and 2 meristic traits (P<0.05). DFA analysis also showed significance differences between the two studied species. Our results refute the synonymy hypothesis of Petroleuciscus ulanus and Alburnus atropatenae on the basis of morphometric and meristic traits. Therefore, based on the results of this study, the taxonomic identities of these two species are valid.
 

 

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