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Showing 2 results for Shabani

Maryam Abbasi, Nader Shabanipour,
Volume 5, Issue 4 (3-2019)
Abstract

In this study, the morphology and organization of the retina of Caspian kutum and fish response to ambient light as retinomotor reaction was investigated. The Rutilus frisii subsp. kutum is an anadromous fish and important native fish specimen of Caspian Sea. The specimens were obtained from Shahid Ansari Teleost Reproduction and Culture center (Guilan province, Iran). For light and dark adaptation fish were left in dark and light at least 30 minute prior to death. The eye retina were dissected out and processed for histological and SEM studies. The eye retina of Caspian kutum like most vertebrates, was composed of ten layers. Two types of photoreceptor cells including rod and short single cone were identified. Sagittal and transverse retinal sections did not show any particular patterns of cone arrangement. The retinomotor response observed in adapted retina to light and dark conditions revealed that Caspian kutum strongly reacts to changing light conditions. A remarkable pigment index indicated kutum relied mostly on rod rather than cone vision. The movement of pigment granules and slight change of cone myoid showed that the fish could recognize colors.


Farhad Mashayekhi, Somaye Shabani, Soheila Talesh Sasani, Prof Zivar Salehi,
Volume 7, Issue 4 (2-2021)
Abstract

Olig1 and Olig2, two transcription factors, play regulatory function in the differentiation and specification of oligodendrocyte progenitor cells (OPCs). In this study the effects of electromagnetic fields (EMF) on total protein concentration ( TPC ) and Olig1 and Olig2 expression in the cerebral cortex of mouse was examined. Twenty-one Balb/c mice were separated into three groups: control, EMF and Sham groups (n=7 for each group). The mice were placed inside the solenoid for a daily EMF exposure of 50 Hz, 1 mT for 6 h/day, 7 days/week for 10 days. The Sham group was also located in the same coil with no exposure. Mice were anesthetized after the final exposure session and their cerebral cortex were collected. TPC and the expression of Olig 1 and Olig2 were studied by Bio-Rad protein assay and western blot, respectively. The cerebral cortex samples were removed for further analysis. There was no significant difference in TPC in the EMF treated cortical samples as compared with those from the SHAM and control groups. It was also shown that the expression of Olig1 and Olig2 was increased in the EMF treated cortical extracts as compared with those in controls and SHAM groups. Therefore, it could be concluded that EMF enhances Olig1 and Olig2 expression in the mice cerebral cortex. Moreover, as Olig1 and Olig2 plays important role in the development of oligodendrocyte progenitor cells, it can be deduced that EMF may affect OPC differentiation by increasing the expression of Olig1 and Olig2. Further studies are needed to clarify the extent of EMF impact on oligodendrocyte differentiation.
 
 

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