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- Azarnoosh Jafari, - Ameneh Assadi Barbariha, - Fereshteh Ghasemzadeh,
Volume 0, Issue 0 (6-2023)

   The present research is floristic study of Imamverdy, Dartum, Ghapagh, Garivan and Niestaneh villages located in 30 km south of Bojnourd (North Khorassan province) with 1442-1783 m altitude. The aim of study was to identify different species, medicinal plants, endemic, vulnerable and low risk species. For this the specimens were collected during March-November 2012 and identified using Flora of Iran and Flora Iranica. The results showed 133 species from 104 genera and 38 families that one of them belonged to Gymnosperms and 132 species were from Angiosperms. Among them, five families, 13 genera and 18 species were from monocotyledons while 32 families, 90 genera and 114 species were reported from dicotyledons.  Also, 40 medicinal plants were introduced from the studied region. Fabaceae, Brassicaceae, Poaceae, Asteraceae, Lamiaceae were the largest families and Astragalus, Poa, Vicia and euphorbia were reports as the largest genera. Erysimum koelzii, Astragalus khoshjailensis, Eryngium bungei, Acanthophyllum pachystegium, Sclerorachis platyrachis, Taraxacum hydrophyllum were endemic elements for Iran and Astragalus ackerbergensis was vulnerable species. Also, Rubia florida, Mentha longifolia, Fumaria vaillanti and Onopordum carmanicum were introduced as low risk species. The maximum and minimum percentage of chorotype were related to Irano-Turanian with 68.14% and Irano-Turanian, Sahra-Arabian regions with 0.7%. The maximum and minimum percentage of life form were reported for hemicryptophytes with 45.8% and cryptophytes with 3% which this result represented cold and arid climate and uncontrolled grazing in the studied region.
Zahra Noormohammadi, Bahar Ghasemzadeh, Farah Farahani,
Volume 5, Issue 1 (6-2018)

Aloe barbadensis is perennial, monocotyledonous, fleshy plant belongs to Aloaceae family. In this study, somoclonal variations of regenerated A. barbadensis plants were investigated. The plantlets of forth subculture transferred to the soil for further study. The genomic DNAs of 40 regenerated plantlets were extracted and genetic variations were studied using SPAR markers including RAPD and ISSR primers. The amounts of Aloe gel also were extracted from regenerated A. vera plants. Average percentage of polymorphism, Shannon index, Nei's genetic diversity and number of effective alleles based on RAPD data were higher than genetic parameters obtained from ISSR data. NJ cluster and STRUCTURE plot based on molecular markers grouped regenerated plants to distinct clusters. AMOVA analysis also showed a significant (P = 0.01) genetic distinction between studied groups. This result also confirmed differentiation of regenerated plants. The amount of Aloe gel in the four groups (based on clustering method) was compared by using analysis of variance (ANOVA). The results showed no significant (P = 0.746) differences between the amount of gel in four group. In total, our findings showed somaclonal variations on genomic level while no significant differences were observed in amount of gel among regenerated Aloe plantlets.

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