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Seyed Milad Hashemi, Somayeh Salmani , Mohammad Hossein Majles Ara,
Volume 2, Issue 4 (3-2016)

It is clear that DNA concentration and biological detection devices have many applications in the fields of genetics and biotechnology. For this goal, some similar devices have been designed working with the laser light which the disadvantages of them are high prices and the use of significant amounts of sample volume. But this designed device which works with LED light has very cheap manufacturing cost. The amounts of DNA used in this device are very low, about a few micro-liters and measurement of small, fast and portable it is also the advantages of these devices. The Data recorded as voltage versus time and the Fourier transform the frequency domain. From the frequency curve, by using the Byrlambrt it is possible to obtain the relative concentrations of DNA, viruses and assays measuring DNA damage and many other applications. By use of this device, the concentration of DNA colored with three different pigments was measured.

Akbar Forghani, Amir Hossein Forghani, Maryam Altafi, Kazem Hashemi Majd, Omid Sofalian,
Volume 8, Issue 3 (10-2021)

Most of agricultural lands in Iran are located in arid and semi-arid regions and are considered as saline soils. In order to investigate the interaction of salinity as well as potassium and calcium on the growth and yield of tomato plants, a factorial experiment was perfected in the form of randomized complete blocks, in hydroponic conditions, with three replicates per treatment. Experimental factors include salinity at three levels (0, 20, and 40 mM NaCl), potassium content form chloride, nitrate (0 and 15 mM), and calcium from chloride, and nitrate (with 0 and 10 mM (. The studied growth factors, including plant height, stem diameter, number of leaves, flowers and fruits and leaf chlorophyll decreased with increase NaCl. Treatment plants with Ca (NO3)2 at 40 mM NaCl, increased the shoot and root dry weight by 55% and 95%, respectively. In addition, application of Ca (NO3)2 in the medium with maximum salinity concentration resulted in an increase of 75% in chlorophyll content. The analysis of data showed that the increase of salinity was accompanied with increase sodium content level of tomato plants. However, the root potassium was observed to decrease. On the contrast to the root, potassium content showed no change in the organs from the root upwards. Also, the use of Ca (NO3)2 with 40 mM NaCl reduced Na+ content by 23% compared with plants treated only with 40 mM NaCl. According to the results, it seems that application of Ca (NO3)2 may improve chlorophyll content, dry weight, and modulate ion hemostasis and decreased the negative of salt stress in tomato plants.

Mohsen Alizadeh, Nezam Armand, Maryam Rahimi, Shokoufeh Haji Hashemi,
Volume 9, Issue 1 (3-2022)

Seaweed extract can improve the physicochemical properties of the soil and has a favorable effect on the plant growth and development due to having high nutrient content, high water holding capacity, plant growth regulators and beneficial microorganisms. The aim of this experiment was to investigate the effect of Ascophyllum nodosum extract, a brown alga, on the morphophysiological characteristics of bean plant (Phaseolus vulgaris) under water stress. The experiment was performed in a Completely Randomized Factorial Design with three replications. Experimental treatments included 4 concentrations of foliar application of seaweed extract (0, 0.2, 0.4 and 0.6%) and 3 levels of water stress including no stress, mild stress and severe stress (irrigation at 75, 50 and 25% of field capacity, respectively). The results showed that the interaction effects of stress and algal extract on the evaluated traits were not significant. However, the seaweed extract significantly increased the leaf area, number of the leaves, leaf length, surface and length of the roots, root dry weight, the content of chlorophyll a and b and the total chlorophyll content. Application of algal extract at levels of 0.2 and 0.4% caused a significant increase in the evaluated parameters as compared with the control plants. The evaluation of water scarcity stress effects showed a significant reduction in the all studied characteristics in accordance with increasing water stress level from 75% to 25% of field capacity. The results of this study showed that foliar application of seaweed extract significantly increased some morphological and photosynthetic properties, while water stress significantly reduced the investigated parameters.

Dr Seyed Reza Hashemi, Eng Negin Akhondpor, Dr Ayoub Farhadi, Eng Elnaz Arabiyan,
Volume 10, Issue 4 (3-2024)

The aim of this study was to investigate the effective genes on apoptosis (BAX and Bcl2) in liver and intestinal cells of broiler chickens fed silver nanoparticles coated on clinoptilolite under acute heat stress induction. 450 d old broiler chicks (Cobb 500) were used in five treatments and six repetitions, and 15 pieces were used in each experimental unit in the form of a completely random design. Experimental diets were: 1) control or basal diet 2) basal diet supplemented by 1% clinoptilolite 3) basal diet supplemented by 1% clinoptilolite coated with 0.5% nanosilver 4) basal diet supplemented by 0.15% organic acid and  5) basal diet supplemented by 1% clinoptilolite coated with 0.5% nanosilver and 0.15% organic acid. Silver nanoparticles coated on clinoptilolite were investigated using XRF and FTIR techniques. In order to induce heat stress, the birds were affected by heat stress for one week in the last week of the breeding period, and on the last day of the stress, liver and intestine samples were obtained to check gene expression. The results of this experiment show that the treatments of clinoptilolite and silver nanoparticles coated on clinoptilolite have an increasing effect on the expression of Bcl2 and Bax, while this effect was not seen in the organic acid treatment. In conclusion, it could be said that if silver nanoparticles are used in feeding livestock and poultry, it is better to use organic acid supplements to reduce the side effects of silver nanoparticles.

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