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Parisa Mohammadi, Sepide Ameri Toorzani, Khadije Kiarostami, Sara Gheravi, Zahra Felahti,
Volume 2, Issue 1 (6-2015)

Total petroleum hydrocarbons (TPHs) is one of the most important environmental challenges. Phytoremediation of oil-polluted soil depends on microorganisms of the rhizosphere. This study attempts to evaluate the microbial impact of Zea mays rhizosphere during a 60 day period using three different treatments: control soil, oil-polluted soil and oil-polluted soil including fertilizer (NH4No3, K2HPO4). Then, the rhizosphere bacteria were identified. The results showed that CFU per gram of control soil was higher than other treated soils, an indication of oil stress on microbial population. In addition, more bacterial diversity was observed in soils and different bacteria were isolated from the soils. Finally, the most resistant bacteria to the oil pollution were assayed. The assay of isolates indicated that all of those separated from the oil-polluted soil have the ability to produce biosurfactant in different quantities. Further investigation must be carried out to optimize the degradation of oil by these isolates. 

Maryam ٍebrahimi, Khadijeh Kiarostami, Zahra Nazem Bokaee,
Volume 5, Issue 4 (3-2019)

Melissa officinalis is a medicinal plant belong to  lamiacea family.  This plant has been cultivated in many parts of the world due to its therapeutic effects. This study was conducted to improve antioxidant activities of proliferated shoots from tissue culture. The seeds of plant   were cultured in MS hormone free medium in order to obtain   seedlings. Then the  shoots were transfer to MS medium supplemented with 2 mg/ L  BAP.After 45 days salicylic acid (SA) was added to the medium at concentrations of 0, 50, 100 and 200 µM. The antioxidant activity and phenolics, flavonoids and rosmarinic acid content in proliferated shoots were evaluated 4,7,10 and 14 days after treatment. As a result the highest free radical scavenging and reducing power activity, as well as the highest value of total phenolic   and rosmarinic acid were observed in shoots   after 14 days of treatment with 100 µM salicylic acid. The elevated level of superoxide anion scavenging activity      and total flavonoids        were obtained from the shoots treated with 50 µM salicylic acid for 14 days. Over all treatment with100 µM salicylic acid for 14 days was better in order to antioxidant activity and compounds with recognized antioxidant activity.

Parichehr Hanachi, Hojat Sadeghi Ali Abadi, Nasim Ghorbani, Roshanak Zarringhalami, Khadijeh Kiarostami,
Volume 8, Issue 1 (6-2021)

From ancient times, plants have been regarded as therapeutic agents, in addition to their usage as food. Plants are rich sources of antioxidant and phenolic compounds. Lavandula angustifolia and Melissa officinalis are medicinal herbs rich in antioxidant compounds. The aim of this study was to compare the antioxidant and anticancer properties of Lavandula angustifolia and Melissa officinalis extracts using aqueous, ethanol and methanol solvents, to select the best extraction methods and solvents and to evaluate the cytotoxic effect of the extracts on HeLa, OVCAR-3 and MCF-7 cancer cell lines. Methanol, aqueous and ethanol extracts were obtained from the dried leaves of the plants and the antioxidant activities of each extract were measured by DPPH and FRAP methods. Finally, the anticancer effects of the extracts on HeLa, OVCAR-3 and MCF-7 cancer cell lines were evaluated by MTT assay in order to identify the most efficient extract. Comparing the results of total antioxidant assay showed that the highest amount belonged to the ethanol extract of Melissa officinalis and Lavandula angustifolia methanol extracts using lyophilization method. The IC50 value of ethanol extract of Melissa officinalis was equal to 0.028 mg/ml on OVCAR-3 cells, which was the best result obtained in comparison with other solvents, and the ethanol extract of Lavandula angustifolia with IC50 = 2.07 mg/ml on MCF-7 cells was the most effective extract among the others. In HeLa cell-line, methanol extract of Lavandula angustifolia with IC50 = 7.36 mg/ml showed the highest cytotoxicity. In this study, for the first time, the effects of different extracts of Lavandula angustifolia and Melissa officinalis on MCF-7, OVCAR-3 and HeLa cancer cells were evaluated and the results showed that ethanol and methanol extracts of these plants had better toxic effect on cancer cells.

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