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Showing 10 results for Cancer

Zahra Eslami Samarin, Mohammad Tahmaseb, Abbas Ghaderi,
Volume 2, Issue 3 (12-2015)

Breast cancer is the most common cancer worldwide and is the second leading cause of death in women after lung cancer. Micro RNAs (miRNAs) are among endogenous factors which are involved in many types of cancers, including breast cancer. Single nucleotide polymorphism (SNP) in the miRNAs, might change their biological activities such as their effects on oncogenes and tumor suppressor genes. Therefore some of miRNA’s SNPs are associated with the risk of different types of cancer, including breast cancer. The aim of this study was to evaluate the correlation between SNP rs11614913 in mir196a2 and the risk of breast cancer. SNP rs11614913 in hsa-miR-196a2 analyzed in 100 breast cancer cases and 100 controls in women living in southern Iran. Polymorphism was identified by the PCR-RFLP method. The PCR product was digested with TaaI restriction enzyme which produced two bands with the length of 187bp and 196bp. The data from this case –control study were analyzed using odds ratios (ORs) with 95% confidence intervals (CIs) to reveal the associations of SNPs in miRNAs with breast cancer susceptibility. The C allele frequencies in patients and controls for miR-196a2 were 0.74 and 0.75, respectively. The genotype frequencies of TT, TC, and CC were 0.05, 0.42, and 0.53 for the patients and 0.06, 0.38, and 0.56 for the controls, respectively. Statistical analysis showed that the genetic frequencies for this SNP were in Hardy-Weinberg equilibrium (p>0.05). The results of this study indicated no significant association between SNP rs11614913 in mir196a2 and the risk of breast cancer. 

Parvin Pishva, Mohammad Tahmaseb, Abbass Ghaderi,
Volume 3, Issue 1 (6-2016)

Lung cancer is the most common cause of death worldwide and result in 1.4 million deaths per year. Lung cancer is the fifth most common cancer in Iran, with rates of 4.7 to 9.2 per 100 thousand populations. Despite the low incidence, survival is not promising. Identification of biological markers and their application in diagnosis of cancer could lead to early diagnosis and therefore longer survival rate in patients. In this study, we evaluated the association of SNP rs11614913 in mir-196a2 with risk of non-small cell lung cancer (NSCLC) in population of southern Iran. SNP rs11614913 in miR-196a2 was assayed in 95 lung cancer patients and 100 controls. Polymorphism was determined by RFLP-PCR. The PCR product was digested with TaaI (5’ ACN^GT) which generated two bands (187bp and 196bp) in TT, three bands (187bp, 196bp and 383bp) in TC and one bandn(383bp) in CC individuals. Analysis of electrophoretic pattern of digestion products revealed that the frequencies of C allele for SNP rs11614913 miR-196a2 were 0.695 in patients and 0.76 in controls. As a result, the genotype frequencies of TT, TC and CC were 0.074, 0.463 and 0.463 in patients and 0.06, 0.36 and 0.58 in controls. The statistical analysis indicates the presence of Hardy-Weinberg equilibrium between the two alleles of the gene in the population studied (p>0.05). Based on the results in this study, no significant association between SNP rs11614913 and susceptibility to NSCLC was found. However, male participants who possess TC/TT genotypes showed high risk for NSCLC compared to those possessed CC genotypes (recessive genetic model).

Hesane Hassanpour, Mohammad Shokrzadeh Lamuki, Reza Tabari, Fatemeh Rezaee, Fatereh Rezaee,
Volume 5, Issue 2 (9-2018)

With the increase of cancer disease and the side-effects of the current treatments, researchers are attempting to find methods with less side-effect. The fern Adiantum capillus-veneris L. has triterpenoid compounds which have anti-tumor characteristics. This study aims to investigate the fatal effect of the extract of this plant on breast cancer (MCF-7) and normal (MRC-5) cell lines using MTT method. A. capillus-veneris specimens were collected from Marzoon Abad Village and were dried at the temperature of 40° C by the use of Soxhlet and Ethanol % 96. Hydroalcoholic extract was produced from the aerial and underground parts of this plant and 3 fractions, i.e., Hexane, Chloroform and Ethyl acetate, were prepared from the extract. The compounds of the plant extract were identified by GC-Mass. The results demonstrate the presence of polyphenolic compounds, terpenoid, fatty acids, wax, alkaloid, N-oxide and fibers, with strong antioxidant effect. The results of the MTT test proved that this extract had a dose-depending fatal effect on the MCF-7 cells and is capable of eradicating the cancer cells. On the other hand, its effect on cancer cells is more than its effect on normal cells. Also, IC50 in both of cell lines induced by the extracts of aerial and underground parts showed a significant difference. The fatal characteristics of the extract are comparable with the cisplatin anti-cancer drug.
Sajjad Farrokyar, Javad Baharara, Tayyebe Ramzani,
Volume 5, Issue 3 (12-2018)

Nowadays natural compounds are increasingly used in various fields since they cause fewer adverse side effects than their chemical counterparts. In addition, preparation and production of natural compounds are comparatively simple. The present study aimed to evaluate the anti-cancer effects of brittle star methanol extract on MCF-7 cells. Anti-cancer effects of brittle star methanol extract were examined on the MCF-7 cell line. Moreover, the toxicity of this compound was assessed using cell apoptosis methods, ethidium bromide/acridine orange (EB/AO) staining and Annexin V Apoptosis Detection Kit. According to the MTT assay, the methanolic extract of brittle star could prevent proliferation in a time- and dose-dependent manner. The calculated inhibitory concentration that decreased the cell-viability by half (IC50) was estimated at 150 µg/ml. On the other hand, results of EB/AO staining were indicative of increased apoptotic cells in the treatment group. In addition, results of Annexin V/PI protocol demonstrated a significant enhancement in the percentage of late apoptotic cells (29.02%) in the treatment groups (150 µg/ml) and the percentage of necrotic cells were 2.77% for a period of 24 hours. According to the results, the methanolic extract of Persian Gulf brittle star induced apoptosis in the MCF-7 cell line. These effects could be attributed to the presence of certain active biological compounds, such as Saponins, Naphthoquinones and Carotenoids. Furthermore, our findings suggested that brittle star might have beneficial effects on cancer management. Therefore, this natural marine compound could be used as a complementary element in the prevention and treatment of cancer.
Katayoon Meimandi, Mohammad Mehdi Yaghoobi,
Volume 6, Issue 1 (5-2019)

In this study, the cytotoxic effects of aqueous and ethanolic extracts of Sedum album L. on human stomach cancer cell line (AGS) and breast cancer cell line (MCF-7) were evaluated by MTT, BrdU and TUNEL assays. The results demonstrated that both extracts had antiproliferative and apoptotic effects in a dose-dependent manner. The MTT assay data revealed that the AGS cell underwent more cytotoxicity in comparison with the MCF-7 cell. It also revealed that ethanolic extract was more potent than aqueous extract. The BrdU assay results showed that the proliferation of AGS and MCF-7 cells was reduced to 50% and 43%, respectively, at the highest concentration of the aqueous extract. In addition, the ethanolic extract reduced the proliferation of AGS and MCF-7 cells to 75% and 60%, respectively. The AGS and MCF-7 cells underwent 52% and 12% apoptotic death upon treatment by the ethanolic extract as TUNEL assay showed. The aqueous extract induced 28% and 25% apoptosis in the AGS and MCF-7 cells, respectively. Both inhibition of proliferation and induction of apoptosis are desirable strategies for cancer treatment among researchers. Identification of S. album compounds and analyzing their effects in animal model of cancer can help us with understanding its anti-cancer properties.


Mina Maleki, Mohammad Pooya Naghshbandi, Zahra Hajihassan,
Volume 6, Issue 4 (1-2020)

Magentotactic bacteria are the types of bacteria capable of orientation in an external magnetic field because of the ability to produce structures called magnetosomes. Magnetosomes, nanometer-scale structures, are present in most of the magnetotactice bacteria. They are intracellular organelles composed of magnetic iron mineral crystals individually surrounded by a phospholipid layer. Because of the unique features of magnetosomes, magentotactic bacteria have become the fascinating subject of research in many research and applied fields of study, including robotics, medicine, biology, environment and geology. In this review, we have tried to introduce magentotactic bacteria, the formation of magnetosomes and their structures, in order to highlight the importance of these bacteria. Finally, some applications of these bacteria in different areas of research, e.g. targeted drug delivery, cancer treatment and removal of heavy metals from water, were described in order that a better understanding of their applications could be obtained.
Parichehr Hanachi, Hojat Sadeghi Ali Abadi, Nasim Ghorbani, Roshanak Zarringhalami, Khadijeh Kiarostami,
Volume 8, Issue 1 (6-2021)

From ancient times, plants have been regarded as therapeutic agents, in addition to their usage as food. Plants are rich sources of antioxidant and phenolic compounds. Lavandula angustifolia and Melissa officinalis are medicinal herbs rich in antioxidant compounds. The aim of this study was to compare the antioxidant and anticancer properties of Lavandula angustifolia and Melissa officinalis extracts using aqueous, ethanol and methanol solvents, to select the best extraction methods and solvents and to evaluate the cytotoxic effect of the extracts on HeLa, OVCAR-3 and MCF-7 cancer cell lines. Methanol, aqueous and ethanol extracts were obtained from the dried leaves of the plants and the antioxidant activities of each extract were measured by DPPH and FRAP methods. Finally, the anticancer effects of the extracts on HeLa, OVCAR-3 and MCF-7 cancer cell lines were evaluated by MTT assay in order to identify the most efficient extract. Comparing the results of total antioxidant assay showed that the highest amount belonged to the ethanol extract of Melissa officinalis and Lavandula angustifolia methanol extracts using lyophilization method. The IC50 value of ethanol extract of Melissa officinalis was equal to 0.028 mg/ml on OVCAR-3 cells, which was the best result obtained in comparison with other solvents, and the ethanol extract of Lavandula angustifolia with IC50 = 2.07 mg/ml on MCF-7 cells was the most effective extract among the others. In HeLa cell-line, methanol extract of Lavandula angustifolia with IC50 = 7.36 mg/ml showed the highest cytotoxicity. In this study, for the first time, the effects of different extracts of Lavandula angustifolia and Melissa officinalis on MCF-7, OVCAR-3 and HeLa cancer cells were evaluated and the results showed that ethanol and methanol extracts of these plants had better toxic effect on cancer cells.

Nina Alizadeh, Shokufeh Malakzadeh,
Volume 8, Issue 2 (7-2021)

The aim of this study was to investigate the interaction modification of curcumin complex molecule (CUR) in beta- and gamma-cyclodextrin (β-CD and γ-CD) carriers with chitosan (CS) nanoparticles for targeted drug delivery and to compare their performance. The targeted drug delivery system includes the therapeutic agent of the CS nanoparticles targeting section of the same drug and the CD carrier system. Calculations of the relationships of the formation of modified complexes and their application were performed using UV-vis spectroscopic data analysis. In this study, spectroscopic spectrum diagrams were drawn to prove the optimization of molecular structure in the modified complexes. Data analysis was performed using their respective equations. The cationic polysaccharide CS, with the presence of amino agents and alcohols along the polysaccharide chains, enables it to form a covalent bond with the complexes and increase the solubility of cyclodextrin. CS nanoparticles strengthen the hydrogen bond by hydrogen bonding and van der Waals hydrogen interactions of the hydroxyl cyclodextrin group with the hydroxyl phenolic group of the drug molecule CUR. Modification of the γ-CD complex with CS shows the strongest interaction with CUR. Both CUR complexes are in the CD-CS host system to transfer the charge from the drug to the carrier and the therapeutic agent. CS nanoparticles have the property of targeted delivery systems for anticancer drugs because the CS external field can be used to direct the drug to specific target cells. The γ-CD-CS host system is the best host as a carrier and therapeutic agent for CUR due to its high solubility and strong interaction.
Leila Gholami, Farnoosh Attari, Mahmood Talkhabi, Fatemeh Saadatpour,
Volume 10, Issue 1 (6-2023)

Breast cancer is the most common cause of death from cancer among women. The triple-negative breast cancer (TNBC) is the most invasive subtype, and chemotherapy is the only therapy option. Cancer cells preferably utilize the glycolysis pathway even with proper oxygen availability, and this activation plays a great role in tumorigenesis. Therefore, glycolysis targeting can be an effective strategy for cancer treatment. Here, the apoptotic effect of a glycolysis inhibitor named dichloroacetate (DCA) on TNBC cells MDA-MB-231 was assessed, and the expression of anti-apoptotic genes and oncogenic miRNAs was evaluated. MTT assay showed that DCA reduces cell viability in a dose-dependent manner with the IC50 concentration of 50 mM. Annexin/PI assay demonstrated that DCA due to DCA treatment. Finally, the expression of anti-apoptotic genes Bcl2l1 and Mcl1 and oncogenic miRNAs miR21 and miR27a decreased due to DCA treatment. Our results confirmed that DCA, as a glycolysis inhibitor, leads to apoptosis induction in TNBC cells because of reducing expression of viability genes and miRNAs.

Negar Khorasani, Javad Baharara, Khadijeh Nejad Shahrokhabadi,
Volume 10, Issue 2 (9-2023)

Pancreatic cancer is one of the most deadly and aggressive cancers; Fluorouracil induces apoptosis and cell cycle arrest in cancer cells. In the present study; the effect of Fluorouracil on different stages of the cell cycle and the expression of genes involved in the internal pathway of apoptosis in the AsPC-1 cell line (human pancreatic cancer) were investigated. In order to do so, MTT assay was used to evaluate the cytotoxic effect of Fluorouracil on AsPC-1 cell proliferation; The type of induced cell death and cell cycle changes were investigated by flow cytometry; changes in the expression level of genes (BAX, Bcl-2, APAF-1, Caspase-3, Caspase-9, p53, p21) were examined by Real-time PCR. Quantitative data were analyzed at the significant level of (p<0.05). The MTT assay results showed that Fluorouracil decreased AsPC-1 cell proliferation in a concentration-dependent manner. The results of flow cytometry analysis showed that increased percentage of apoptotic cells in the treated cells; Fluorouracil induces S phase cell cycle arrest in AsPC-1 cells and reduced distribution in the G1 phase. The Real-time PCR results in treated cells showed an increase in the expression of genes in the mitochondrial apoptotic pathway as well as genes effective in regulating the cell cycle. Fluorouracil reduces cell proliferation and induces apoptosis by increasing the expression of genes involved in the Intrinsic apoptotic pathway in AsPC-1 cells; Fluorouracil also caused cell cycle arrest in these cells by regulating the (p53, p21) genes.

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