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Behnam Hamzehee, Maryam Nobakht, Younes Asri, Gholamreza Bakhshi Khaniki,
Volume 4, Issue 4 (3-2018)
Abstract

The genus Crataegus L. has a wide distribution in Iran. Induviduals of Crataegus meyeri Pojark. show morphological variations in different regions. In this research, the effects of climatic variations on morphological traits of C. meyeri were studied. Seventy five specimens collected from nine provinces of Iran were measured in two sets of quantitative and qualitative traits. Statistical analysis of climatic data and morphological traits showed that wind and temperature were the most effective factors affecting the variety of morphological traits including leaf blade of short shoot and flowering shoot, indumentum of fruit surface and fruit shape. Relative humidity has also been correlated with the shape of the fruit base. Quantitative traits of leaf blade of short shoots and flowering shoots showed the highest correlation and leaf blade of elongate shoots with the least statistical correlation with climatic data.
Somayeh Keypour, Hossein Riahi, Mostafa Ebadi, Ali Borhani, Mohammad Reza Asef Shayan, Naser Safaie,
Volume 5, Issue 4 (3-2019)
Abstract

The genus Ganoderma belongs to Polyporales, well-known for its medicinal properties and as a plant pathogen. The aim of this study was to identify the genetic variation between populations of two Ganoderma lucidum Karst. complex isolates using RAPD. For this purpose, four populations of G. resinaceum Boud. and eight populations of G. lucidum collected and purified during 2011-2012, were studied for the first time in Iran. Ten random primers were used for RAPD reaction. The result for RAPD-PCR studies of G. lucidum complex showed a wide range of diversity, as scores of 61.48 and 40.16 were estimated for the inter-specific diversity of G. lucidum and G. resinaceum, respectively. Cluster analysis confirmed the separation of two species populations in different clusters. In the analysis of genetic structure, two probable groups (K = 2) were identified in the studied germplasm which was also confirmed by the results of bar plot.
 

Mitra Mohammadi Bazargani,
Volume 5, Issue 4 (3-2019)
Abstract

Epilobium minutiflorum belongs to the genus Epilobium with important medicinal properties. In this study, the natural variation of phytochemical compounds of E. minutiflorum at different altitudes has been investigated. Aerial parts of plants were collected at flowering stage from natural habitats at different altitudes of 2387, 2569 and 2813 masl. The plant extracts were obtained with 80% methanol and several phytochemical properties were measured. The properties included the total flavonoid content (TFL), total phenol (TPH), antioxidant capacity (AOX) and anthocyanin content (ACY) measured by the AlCl3, the Folin-Ciocalteu, the Ferric reducing antioxidant power (FRAP) and the differential PH methods, respectively. In addition, phytochemical components were identified by the GC/MS method. The results of photometric analysis indicated that the population of Shemshak, located at the highest altitude showed the highest content of TFL, TPH and AOX with 30.39 mg/gr DW, 938.91 mg/gr DW and 77.77 mM/gr DW, respectively. The TFL, TPH and AOX values showed a positive correlation with altitude. The results of GC/MS analysis revealed the presence of 41 compounds in E. minutiflorum, which were separated into flavonoids, phenolic acid and its derivatives, steroids and terpenes groups. Shemshak population showed the highest value in all identified compounds. A positive correlation was also observed between altitude and 30 compounds identified by the GC/MS method including mainly flavonoids and phenolics. The results of this study indicated that environmental factors at higher altitudes may influence the elevation of the flavenols and phenol content in E. minutiflorum. The identification of these influential factors on phytochemical content at higher altitudes requires further studies.

 
 

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