en Evaluating the effect of ozone in reducing the toxicity of corn contaminated with ochratoxin A in Wistar rats علوم جانوری Animal Biology مقاله پژوهشی Original Article <div dir="ltr" style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:14.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt">Objective</span></b><span style="font-size:9.0pt">: Ozone treatment has been recognized as an effective approach to significantly reduce mycotoxin levels, including ochratoxin A (OTA), in agricultural products. This study aimed to evaluate the safety of untreated and ozone-treated OTA-contaminated corn (OCC) through a sub-chronic toxicity assessment in rats.</span><br /> <b><span style="font-size:9.0pt">Method</span></b><span style="font-size:9.0pt">: Male rats were randomly assigned into one control group and three experimental diet groups. The experimental groups received untreated OCC or ozone-treated OCC through oral administration for a 30-day period. Clinical signs, survival, hematological parameters, serum biochemical indices, and histopathological alterations of liver and kidney tissues were examined to evaluate potential toxicological effects. </span></span></span></span><br /> <b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Results</span></span></b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">: No mortality or overt clinical abnormalities were observed during the experimental period. Rats fed untreated OCC exhibited significant decreases in white blood cell (WBC) counts and marked elevations in alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) levels. Histopathological evaluation revealed OTA-induced lesions in both liver and kidney tissues. In contrast, rats fed ozone-treated OCC showed reduced biochemical alterations and attenuated histopathological damage compared with those receiving untreated OCC.</span></span></div> <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:14.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt">Objective</span></b><span style="font-size:9.0pt">: Ozone treatment has been recognized as an effective approach to significantly reduce mycotoxin levels, including ochratoxin A (OTA), in agricultural products. This study aimed to evaluate the safety of untreated and ozone-treated OTA-contaminated corn (OCC) through a sub-chronic toxicity assessment in rats.</span><br /> <b><span style="font-size:9.0pt">Method</span></b><span style="font-size:9.0pt">: Male rats were randomly assigned into one control group and three experimental diet groups. The experimental groups received untreated OCC or ozone-treated OCC through oral administration for a 30-day period. Clinical signs, survival, hematological parameters, serum biochemical indices, and histopathological alterations of liver and kidney tissues were examined to evaluate potential toxicological effects. </span></span></span></span><br /> <b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">Results</span></span></b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">: No mortality or overt clinical abnormalities were observed during the experimental period. Rats fed untreated OCC exhibited significant decreases in white blood cell (WBC) counts and marked elevations in alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) levels. Histopathological evaluation revealed OTA-induced lesions in both liver and kidney tissues. In contrast, rats fed ozone-treated OCC showed reduced biochemical alterations and attenuated histopathological damage compared with those receiving untreated OCC. </span></span><br /> &nbsp;</div> Biochemisty Fungi toxins, Histopathology, in vivo toxicity, wistar rat Biochemisty Fungi toxins, Histopathology, in vivo toxicity, wistar rat 0 0 http://nbr.khu.ac.ir/browse.php?a_code=A-10-1676-1&slc_lang=en&sid=1 Maryamsadat Mesbahi bidgoli Maryamsadat Mesbahi bidgoli maryam.mesbahi@isfahan.pnu.ac.ir 100319475328460011625 100319475328460011625 No Department of Biology, Payame Noor University, ،Tehran, Iran Department of Biology, Payame Noor University, ،Tehran, Iran Mohammad Fazilati Mohammad Fazilati mfazilati@pnu.ac.ir 100319475328460011626 100319475328460011626 No Department of Biology, Payame Noor University, Tehran, Iran Department of Biology, Payame Noor University, Tehran, Iran Anosheh Rahmani Anosheh Rahmani .rahmani@standard.ac.ir 100319475328460011627 100319475328460011627 Yes Department of Food, Halal and Agricultural Products, Food Technology and Agricultural Products Research Center, Standard Research Institute (SRI), Karaj, Iran. Department of Food, Halal and Agricultural Products, Food Technology and Agricultural Products Research Center, Standard Research Institute (SRI), Karaj, Iran. Habibollah Nazem Habibollah Nazem h_nazem@pnu.ac.ir 100319475328460011628 100319475328460011628 No Department of Biology, Payame Noor University, Tehran, Iran Department of Biology, Payame Noor University, Tehran, Iran