Volume 21, Issue 26 (12-2023)
RSMT 2023, 21(26): 89-102 |
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salehpour M, sarlak A, samadi H. The Effect Of Eight Weeks Of Endurance Training On The Expression Of Glutathione Peroxidase 4 And Malondialdehyde In The Soleus Muscle Of Aged Aale Wistar Rats. RSMT 2023; 21 (26) :89-102
URL: http://jsmt.khu.ac.ir/article-1-584-en.html
URL: http://jsmt.khu.ac.ir/article-1-584-en.html
Shahid Rajaee Teacher Training University , salehpour57@gmail.com
Abstract: (2528 Views)
Introduction: The increase of iron due to aging affects the process of ferroptosis, and the final product of lipid peroxidation, MDA, is the central regulator of ferroptosis. Therefore, the aim of the research was to determine the effect of eight weeks of endurance training on the expression of GPX4 and MDA in the soleus muscle of aged male rats.
Materials and Methods: This study is of an experimental type. 20 healthy rats were divided into two groups of 10 each: training group and control group. The training was done incrementally for eight weeks. 48 hours after the last training session, the soleus muscle of mice was surgically removed and the amount of variables was measured by gene expression and ELISA methods.
Results: There was no significant difference in the level of GPX4 gene expression in the soleus muscle of exercise group rats compared to control group rats (p=0.4079). But the amount of MDA in the training group was significantly reduced compared to the control group (p=0.0028).
Conclusion: According to the findings of the present research, it seems that the effect of endurance training in this research shows a decrease in ferroptosis. The absence of a significant difference in the level of GPX4 gene expression is a sign that endurance training with this intensity does not affect it.
Materials and Methods: This study is of an experimental type. 20 healthy rats were divided into two groups of 10 each: training group and control group. The training was done incrementally for eight weeks. 48 hours after the last training session, the soleus muscle of mice was surgically removed and the amount of variables was measured by gene expression and ELISA methods.
Results: There was no significant difference in the level of GPX4 gene expression in the soleus muscle of exercise group rats compared to control group rats (p=0.4079). But the amount of MDA in the training group was significantly reduced compared to the control group (p=0.0028).
Conclusion: According to the findings of the present research, it seems that the effect of endurance training in this research shows a decrease in ferroptosis. The absence of a significant difference in the level of GPX4 gene expression is a sign that endurance training with this intensity does not affect it.
Type of Study: Research |
Subject:
sport physiology
Received: 2023/06/3 | Accepted: 2023/10/14 | Published: 2023/12/31
Received: 2023/06/3 | Accepted: 2023/10/14 | Published: 2023/12/31
References
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2. Safarkhanlou H, Rezaei Ghahroodi Z. The evolution of the elderly population in Iran and the world. Statistics J. 2017;.16-8:(3)5
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10. Huang Y, Wu B, Shen D, Chen J, Yu Z, Chen C. Ferroptosis in a sarcopenia model of senescence accelerated mouse prone 8 (SAMP8). International Journal of Biological Sciences. 2021;17(1):151. [DOI:10.7150/ijbs.53126]
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13. Sahra Gard H, Rajabi H. Comparison of acute and chronic effects of high-intensity interval training in warm and normal environments on the serum levels of glutathione peroxidase and HSP72 in active men. Physiology of exercise and physical activity journal. 2015;8(1):1201-12[in Persian ].
14. Yang WS, SriRamaratnam R, Welsch ME, Shimada K, Skouta R, Viswanathan VS, et al. Regulation of ferroptotic cancer cell death by GPX4. Cell. 2014;156(1-2):317-31. [DOI:10.1016/j.cell.2013.12.010]
15. Weiland A, Wang Y, Wu W, Lan X, Han X, Li Q, et al. Ferroptosis and its role in diverse brain diseases. Molecular neurobiology. 2019;56(7):4880-93. [DOI:10.1007/s12035-018-1403-3]
16. Ju J, Song Y-n, Wang K. Mechanism of Ferroptosis: A Potential Target for Cardiovascular Diseases Treatment. Aging and disease. 2021;12(1):261. [DOI:10.14336/AD.2020.0323]
17. Fazelzadeh M, Fallah Mohammadi Z. The effect of 4 weeks of plyometric exercises on changes in the serum levels of brain-derived nutritional factor, MDA and SOD in active men. Physiology of exercise and physical activity journal. 2016;9(2):1447-54[in Persian ].
18. Liu T, Cui Y, Dong S, Kong X, Xu X, Wang Y, et al. Treadmill Training Reduces Cerebral Ischemia-Reperfusion Injury by Inhibiting Ferroptosis through Activation of SLC7A11/GPX4. Oxidative Medicine and Cellular Longevity. 2022. [DOI:10.1155/2022/8693664]
19. Son YH, Lee S-M, Lee SH, Yoon JH, Kang JS, Yang YR, et al. Comparative molecular analysis of endurance exercise in vivo with electrically stimulated in vitro myotube contraction. Journal of Applied Physiology. 2019;127(6):1742-53. [DOI:10.1152/japplphysiol.00091.2019]
20. Moradi, Habibi, Shakerian, Radiant. The effect of continuous and periodic aerobic exercise on malondialdehyde, dopamine and glutathione peroxidase levels in the hippocampus of rats with pseudo-Parkinsonism. Jundishapur Medical Scientific Journal. 2020;19(2):187-201[in Persian ].
21. Lu Y, Wiltshire HD, Baker JS, Wang Q. Effects of High Intensity Exercise on Oxidative Stress and Antioxidant Status in Untrained Humans: A Systematic Review. Biology. 2021;10(12):1272. [DOI:10.3390/biology10121272]
22. Yu Y, Niu R, Li R, Wang J, Cao Q, Sun Z. EFFECTS OF PHYSICAL EXERCISE ON FLUORIDE-INDUCED OXIDATIVE STRESS IN THE LIVER OF MICE. Fluoride. 2021;54(1):43-57.
23. Ebrahimi M, Huanlu DF, Hedayati DM. The effect of different time periods of endurance training on the activity of antioxidant enzymes in rat serum. Researcher's Journal. 2013;18(1):16-22[in Persian ].
24. Ighodaro O, Akinloye O. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria journal of medicine. 2018;54(4):287-93. [DOI:10.1016/j.ajme.2017.09.001]
25. Song X, Wang X, Liu Z, Yu Z. Role of GPX4-mediated ferroptosis in the sensitivity of triple negative breast cancer cells to gefitinib. Frontiers in oncology. 2020;10:597434. [DOI:10.3389/fonc.2020.597434]
26. Xiang W, Yi X, Xue-Hai Z, Ding-Sheng J. Posttranslational Modifications in Ferroptosis. Oxidative Medicine and Cellular Longevity. 2020;2020. [DOI:10.1155/2020/8832043]
27. Forcina GC, Dixon SJ. GPX4 at the crossroads of lipid homeostasis and ferroptosis. Proteomics. 2019;19(18):1800311. [DOI:10.1002/pmic.201800311]
28. Radak Z, Chung HY, Goto S. Systemic adaptation to oxidative challenge induced by regular exercise. Free Radical Biology and Medicine. 2008;44(2):153-9. [DOI:10.1016/j.freeradbiomed.2007.01.029]
29. Devi SA, Kiran TR. Regional responses in antioxidant system to exercise training and dietary vitamin E in aging rat brain. Neurobiology of aging. 2004;25(4):501-8. [DOI:10.1016/S0197-4580(03)00112-X]
30. Babak HM, Seyedreza AH, Reza KM, Taimaz D. The effect of eight weeks of aerobic training with spirulina supplementation on the plasma levels of MDA, SOD and TAC in men with type 2 diabetes [in Persian ].
31. Zahra SA, Masoume H. The effect of aerobic training on renal levels of apelin and nitric oxide in kidney tissue of male rats exposed to zinc oxide nanoparticles [in Persian ].
32. Fatot, Jalali, Taghian, Farzaneh. The effect of eight weeks of aerobic training with nano eugenol supplementation on pancreatic tissue UPC3 gene expression and MDA serum levels in diabetic rats. Research in sports science and medicinal plants. 2020;1(1):79-89[in Persian ].
33. Fazelzadeh, Mohammadi F, Zia. The effect of 4 weeks of plyometric training on changes in serum levels of brain-derived nutritional factor, MDA and SOD in active men. Journal of Exercise Physiology and Physical Activity. 2016;9(2):14 47-54([in Persian ].
34. Ameri G, Govari F, Nazari T, Rashidinejad M, Afsharzadeh P. The adult age theories and definitions. Hayat Journal. 2002;8(1):4-13.
35. Safarkhanlou H, Rezaei Ghahroodi Z. The evolution of the elderly population in Iran and the world. Statistics J. 2017;.16-8:(3)5
36. Aram S, Ezatollah. Elderly issues in Iran. Social Science Quarterly. 1992;1(1.2):113-28[in Persian ].
37. Mirzai, Qahfarkhi S, Mehri. Demography of the elderly in Iran based on the censuses of 1325 to 1385. Salamand: Iranian Journal of Geriatrics. 2007;2(3):326-31 [in Persian ].
38. Allen T, Anderson E, Langham W. Total body potassium and gross body composition in relation to age. Journal of Gerontology. 1960;15:348-57. [DOI:10.1093/geronj/15.4.348]
39. Curcio F, Ferro G, Basile C, Liguori I, Parrella P, Pirozzi F, et al. Biomarkers in sarcopenia: a multifactorial approach. Experimental gerontology. 2016;85:1-8. [DOI:10.1016/j.exger.2016.09.007]
40. Morley JE, Baumgartner RN, Roubenoff R, Mayer J, Nair KS. Sarcopenia. Journal of Laboratory and Clinical Medicine. 2001;137(4):231-43. [DOI:10.1067/mlc.2001.113504]
41. Rosenberg IH. Sarcopenia: origins and clinical relevance. The Journal of nutrition. 1997;127(5):990S-1S. [DOI:10.1093/jn/127.5.990S]
42. Behrad, Hamed, farmer a, Borujani B, Asadi, subspecies, et al. Sarcopenia disease and recovery strategies: a review article. Journal of Faculty of Medicine, Tehran University of Medical Sciences. 2020;78(10):632-43[in Persian ].
43. Huang Y, Wu B, Shen D, Chen J, Yu Z, Chen C. Ferroptosis in a sarcopenia model of senescence accelerated mouse prone 8 (SAMP8). International Journal of Biological Sciences. 2021;17(1):151. [DOI:10.7150/ijbs.53126]
44. Freiberger E, Sieber C, Pfeifer K. Physical activity, exercise, and sarcopenia-future challenges. Wiener Medizinische Wochenschrift. 2011;161(17):416-25. [DOI:10.1007/s10354-011-0001-z]
45. Mou Y, Wang J, Wu J, He D, Zhang C, Duan C, et al. Ferroptosis, a new form of cell death: opportunities and challenges in cancer. Journal of hematology & oncology. 2019;12(1):1-16. [DOI:10.1186/s13045-019-0720-y]
46. Sahra Gard H, Rajabi H. Comparison of acute and chronic effects of high-intensity interval training in warm and normal environments on the serum levels of glutathione peroxidase and HSP72 in active men. Physiology of exercise and physical activity journal. 2015;8(1):1201-12[in Persian ].
47. Yang WS, SriRamaratnam R, Welsch ME, Shimada K, Skouta R, Viswanathan VS, et al. Regulation of ferroptotic cancer cell death by GPX4. Cell. 2014;156(1-2):317-31. [DOI:10.1016/j.cell.2013.12.010]
48. Weiland A, Wang Y, Wu W, Lan X, Han X, Li Q, et al. Ferroptosis and its role in diverse brain diseases. Molecular neurobiology. 2019;56(7):4880-93. [DOI:10.1007/s12035-018-1403-3]
49. Ju J, Song Y-n, Wang K. Mechanism of Ferroptosis: A Potential Target for Cardiovascular Diseases Treatment. Aging and disease. 2021;12(1):261. [DOI:10.14336/AD.2020.0323]
50. Fazelzadeh M, Fallah Mohammadi Z. The effect of 4 weeks of plyometric exercises on changes in the serum levels of brain-derived nutritional factor, MDA and SOD in active men. Physiology of exercise and physical activity journal. 2016;9(2):1447-54[in Persian ].
51. Liu T, Cui Y, Dong S, Kong X, Xu X, Wang Y, et al. Treadmill Training Reduces Cerebral Ischemia-Reperfusion Injury by Inhibiting Ferroptosis through Activation of SLC7A11/GPX4. Oxidative Medicine and Cellular Longevity. 2022. [DOI:10.1155/2022/8693664]
52. Son YH, Lee S-M, Lee SH, Yoon JH, Kang JS, Yang YR, et al. Comparative molecular analysis of endurance exercise in vivo with electrically stimulated in vitro myotube contraction. Journal of Applied Physiology. 2019;127(6):1742-53. [DOI:10.1152/japplphysiol.00091.2019]
53. Moradi, Habibi, Shakerian, Radiant. The effect of continuous and periodic aerobic exercise on malondialdehyde, dopamine and glutathione peroxidase levels in the hippocampus of rats with pseudo-Parkinsonism. Jundishapur Medical Scientific Journal. 2020;19(2):187-201[in Persian ].
54. Lu Y, Wiltshire HD, Baker JS, Wang Q. Effects of High Intensity Exercise on Oxidative Stress and Antioxidant Status in Untrained Humans: A Systematic Review. Biology. 2021;10(12):1272. [DOI:10.3390/biology10121272]
55. Yu Y, Niu R, Li R, Wang J, Cao Q, Sun Z. EFFECTS OF PHYSICAL EXERCISE ON FLUORIDE-INDUCED OXIDATIVE STRESS IN THE LIVER OF MICE. Fluoride. 2021;54(1):43-57.
56. Ebrahimi M, Huanlu DF, Hedayati DM. The effect of different time periods of endurance training on the activity of antioxidant enzymes in rat serum. Researcher's Journal. 2013;18(1):16-22[in Persian ].
57. Ighodaro O, Akinloye O. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria journal of medicine. 2018;54(4):287-93. [DOI:10.1016/j.ajme.2017.09.001]
58. Song X, Wang X, Liu Z, Yu Z. Role of GPX4-mediated ferroptosis in the sensitivity of triple negative breast cancer cells to gefitinib. Frontiers in oncology. 2020;10:597434. [DOI:10.3389/fonc.2020.597434]
59. Xiang W, Yi X, Xue-Hai Z, Ding-Sheng J. Posttranslational Modifications in Ferroptosis. Oxidative Medicine and Cellular Longevity. 2020;2020. [DOI:10.1155/2020/8832043]
60. Forcina GC, Dixon SJ. GPX4 at the crossroads of lipid homeostasis and ferroptosis. Proteomics. 2019;19(18):1800311. [DOI:10.1002/pmic.201800311]
61. Radak Z, Chung HY, Goto S. Systemic adaptation to oxidative challenge induced by regular exercise. Free Radical Biology and Medicine. 2008;44(2):153-9. [DOI:10.1016/j.freeradbiomed.2007.01.029]
62. Devi SA, Kiran TR. Regional responses in antioxidant system to exercise training and dietary vitamin E in aging rat brain. Neurobiology of aging. 2004;25(4):501-8. [DOI:10.1016/S0197-4580(03)00112-X]
63. Babak HM, Seyedreza AH, Reza KM, Taimaz D. The effect of eight weeks of aerobic training with spirulina supplementation on the plasma levels of MDA, SOD and TAC in men with type 2 diabetes [in Persian ].
64. Zahra SA, Masoume H. The effect of aerobic training on renal levels of apelin and nitric oxide in kidney tissue of male rats exposed to zinc oxide nanoparticles [in Persian ].
65. Fatot, Jalali, Taghian, Farzaneh. The effect of eight weeks of aerobic training with nano eugenol supplementation on pancreatic tissue UPC3 gene expression and MDA serum levels in diabetic rats. Research in sports science and medicinal plants. 2020;1(1):79-89[in Persian ].
66. Fazelzadeh, Mohammadi F, Zia. The effect of 4 weeks of plyometric training on changes in serum levels of brain-derived nutritional factor, MDA and SOD in active men. Journal of Exercise Physiology and Physical Activity. 2016;9(2):14 47-54([in Persian ].
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