Showing 2 results for Choobineh
Kia Salimi, Ali Asghar Ravasi, Siroos Choobineh, Kamran Rakhshan ,
Volume 0, Issue 0 (11-2019)
Abstract
Abstract
Introduction and purpose: Structural remodeling of the heart following myocardial infarction often leads to heart failure, a condition characterized by impaired cardiac function and increased hemodynamic load. Inflammation, oxidative stress, and ventricular remodeling play key roles in the pathogenesis of heart failure. This study aimed to investigate the effects of combined administration of propionate and low-volume HIIT on inflammatory status and cardiac function in mice with heart failure.
Methodology: Forty male Wistar rats (8 weeks old, 200–250 g) were induced with HF by isoproterenol injection (130 mg/kg) and randomly assigned to five groups: (1) Control, (2) heart failure, (3) heart failure + Propionate, (4) heart failure + HIIT, and (5) heart failure + HIIT + Propionate. HIIT consisted of 10 one-minute bouts with a 1:1 work-to-rest ratio, performed over 6 weeks. Propionate was administered orally at a dose of 1000 mg/kg prior to each training session. Cardiac function was assessed by echocardiography, and cytokine levels were measured using ELISA. Data were analyzed using one-way ANOVA (p<0.05).
Findings: Both HIIT and propionate individually improved cardiac function and inflammatory markers. The combined intervention significantly increased ejection fraction (p = 0.038) and fractional shortening (p=0.023), reduced TNF-α (p=0.0061), and increased IL-10 levels (p=0.0007), yielding superior outcomes compared with individual treatments.
Discussion and conclusion: This study demonstrates that the combination of propionate supplementation with low-volume HIIT effectively improves inflammation and cardiac function in rats with heart failure.
Younes Sarkabood, Mohamadreza Kordi, Siroos Choobineh, Fatemeh Nasery,
Volume 0, Issue 0 (11-2019)
Abstract
Abstract
Introduction: Oxidative stress and neuroinflammation are key mechanisms in the pathophysiology of epilepsy. Physical activity plays a role in controlling and improving epileptic symptoms. This study aimed to investigate the effects of moderate-intensity continuous training (MICT) on the activity of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), the levels of HMGB1 and IL-10 proteins, and seizure severity in the hippocampus of epileptic rats.
Methods: Thirty-two male Wistar rats (6–8 weeks old; mean body weight: 226.625 ± 14.966 g) were randomly assigned to four groups: epilepsy, control, sham, and epilepsy + MICT. After completion of the 8-week training protocol, SOD and GPx activities were measured by ELISA, hippocampal HMGB1 and IL-10 levels were assessed by Western blotting, and seizure severity was evaluated using the Racine scale. Data were analyzed using one-way analysis of variance (ANOVA) and the Mann–Whitney U test in SPSS.
Results: Epilepsy induction led to decreased SOD (p < 0.02) and GPx (p < 0.001) activities, increased HMGB1 levels (p < 0.001), decreased IL-10 levels (p < 0.001), and increased seizure severity. MICT increased GPx activity (p < 0.048) and reduced HMGB1 levels (p < 0.001) in the training group compared with the epilepsy group. Although SOD activity (p = 0.953) and IL-10 levels (p = 0.198) did not change significantly, seizure severity was significantly reduced in the trained group.
Conclusion: Moderate-intensity continuous training appears to exert protective effects and reduce seizure severity in epileptic rats by strengthening the antioxidant defense system and attenuating neuroinflammation.
