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A Gorzi, H Rajabi, R Gharakhanlou, M.r Dehkhoda, M Hedayati,
Volume 15, Issue 13 (9-2017)
Abstract

The purpose of this study was to investigate the effects of 8 weeks of resistance training (RT) on activity of total and A12 Type of acetyl cholinesterase in Soleus muscles of rats. 16 male wistar rats provided from Razi institute (age: 10 weeks and weight: 172.415±7.090 gr), were randomly divided to 2 groups (Control; n=8 and RT; n=8). Training group carried out 8 weeks (5 session/week) of resistance training on 1-meter height ladder (divided by 26 stairs) with loading 30% of their body weight (suspended from the tail) in the first week which was gradually increased to 200% in the last week. Training included 3 sets of 4 reps with 3 min rest between sets. 48 hrs after last session of training, Sol muscles of animals moved out under sterilized situation by cutting on posterio-lateral side of hind limb. For separating AchE subunits, we used from homogenization and electrophoresis (0/06 non-denaturaing Polyacrilamide). Acetyl cholinesterase activity was measured by Elisa kit. Independent t-test showed that there was no significant differences between training and control groups in both total (p=0.262) and A12 forms (p=0.246) of AchE in soleus muscle. The reason for insignificant differences in acetyl cholinesterase of soleus might be indicative of no complete involvement of this muscle in this type of training and therefore no responsiveness of acetyl cholinesterase activity of this muscle following resistance training. However, this should be studied in future with higher volume and intensity of training. Because the increases in AchE content by training is not excluded yet.


A Gorzi, R Jazaei, A Rahmani, A Bahari,
Volume 17, Issue 18 (12-2019)
Abstract

Determining the best rest interval durations between resistance exercise sets for adaptation is very important. This study investigated the effect of different rest intervals duration between resistance exercise (RE) sets on the gene expression of CGRP and IGF-1. Forty two male Wistar rats were randomly divided in to 7 groups. The resistance exercise included one session of climbing on one meter ladder with 26 steps. Exercise included 4 sets of 5 repetitions with an overload of 150 percent of the rat's body weight and 30, 60, 90, 120, 150 and 180 seconds rest intervals. The qRT-PCR technique was used to evaluate the gene expression of CGRP and IGF-1. The gene expression of CGRP in soleus muscles following RE with rest intervals of 30 (P=0.001), 60 (P=0.001) and 90 seconds (P=0.001) were significantly lower than 120, 150 and 180 seconds. However, there were no significant differences in the gene expression of IGF-1 among all groups (P=0.12). Based on our results, it seems that these rest interval domains can be addressed as a cut point of gene expression for the strength and hypertrophy developing process at the cellular level. 

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