Mrs Elmira Ahmadi, Dr Shahnaz Shahrbanian, Dr Mohamadreza Kordi, Dr Reza Gharakhanlou, Dr Gregory Pearcey,
Volume 0, Issue 0 (11-2019)
Abstract
Fatigue is one of the most common symptoms among individuals with multiple sclerosis (MS). The imbalance of inflammatory cytokines in the brain causes mental fatigue. It is believed that aerobic exercise may moderate the level of inflammation of cytokines and thus reduce mental fatigue. The present study was conducted to investigate the effect of swimming aerobic training on pro-inflammatory factors (interleukin 6) and anti-inflammatory factors (interleukin 10) to evaluate mental fatigue in the brain of female mice after EAE induction. In this research, 21 mice (8 weeks old, 18 to 20 grams) were divided into 3 groups: healthy control, EAE control, and EAE swimming aerobic exercise. The animals in the training group performed moderate-intensity swimming exercises for 4 weeks, 5 sessions per week, 30 minutes per session. The western blot method was used to analyze the research variables. One-way analysis of variance and Tukey's post hoc test were used for statistical analysis. The results showed that there was no significant difference in the amount of IL-6 between the EAE group and swimming training, but the amount of IL10 in the swimming training group showed a significant increase compared to the EAE group. However, the ratio of IL 10/IL 6 values between the swimming training group with EAE was also not significant. It seems that moderate-intensity swimming aerobic exercises, without change in the pro-inflammatory factor, along with driving towards anti-inflammatory factors, with an increase in IL10, can lead to an improvement in the balance of the inflammatory index and a reduction in fatigue between the EAE group and swimming exercise.
Samira Emadi, Shahnaz Shahrbanian, Mohammadreza Kordi, Mahdieh Molanouri Shamsi,
Volume 0, Issue 0 (11-2019)
Abstract
Disruption of the homeostasis of immune regulatory mediators Aryl hydrocarbon receptor (AhR) and Ornithine decarboxylase-1(ODC-1) is involved in the pathogenesis of multiple sclerosis (MS). Physical activities play a role in controlling and improving the symptoms of MS. This study was conducted in order to investigate the effect of 4 weeks of physical activity in an enriched environment on the gene expression of AhR and ODC-1 in the cerebellum tissue of an MS mice model. This experimental study was conducted on 30 female C57BL/6 mice. Mice were divided into 3 groups of 10 including 1) healthy control, 2) experimental model of MS and 3) physical activity in enriched environment. After induction of experimental autoimmune encephalomyelitis (EAE), the third group lived in the enriched environment for 4 weeks. Body weight and clinical score were recorded daily from induction until 28 days. The gene expression of AhR and ODC-1 in the cerebellum was measured by RT-PCR method. Statistical analysis of the data was performed using one-way analysis of variance at a significance level of less than 0.05. Activity in the enriched environment significantly decreased the gene expression of AhR compared to the EAE group (P=0/02) and also decreased the gene expression of ODC-1, but it was not statistically significant (P=0/28). Also, the clinical score improved significantly after activity in the enriched environment (P= 0/001). Lifestyle change and activity in the enriched environment led to the down regulation of the gene expression of AhR and ODC-1, and it improved the clinical score of animals.
