Dr Sara Zare Karizak, Mis Zaynab Teimouri, Dr Abdosaleh Zar,
Volume 0, Issue 0 (11-2019)
Abstract
Introduction
High-Intensity exercise is accompanied by inflammation and oxidative stress. This study aimed to investigate the effect of the FIFA test with curcumin supplementation on serum MDA, TAC, and CRP in male football assistant referees.
Method
In this semi-experimental study, 20 football assistant referees (mean age 28.56±3.75 years, height 179±4.21 cm, weight 72.78±7.08 kg) were selected voluntarily then were randomly assigned to the curcumin + FIFA test and the FIFA test groups. The test + supplement group, in addition to FIFA tests, received 5 grams of curcumin daily (2.5gram at morning and 2.5 gram at evening) for five days. Blood sampling was performed in three stages (before supplementation, two and a half day before, immediately after, and 48 hours after the FIFA Interval Physical Fitness Test) and serum levels of variables were measured using the spectrophotometric method. Repeated-measures ANOVA and Bonferoni post hoc tests were performed in SPSS26.
Results
There was significant increase in serum CRP, MDA, and TCA in both groups in the first post-test (P=0.000, P=0.041, P=0.008, P=0.009, P= 0.048, P=0.014). Also, in the second post-test of the test group, the increase in CRP and MDA levels remained significant (P = 0.000, P = 0.022). However, TAC became a non-significant increase (P=0.160), while its increase in the test + supplement group was still significant (P=0.013), and in the test + supplement group, MDA became a non-significant increase compared to the pre-test (P=0.186), while in the test group it still had a significant increase (P=0.022). In addition, there was a significant difference between the groups in CRP and TAC values (P=0.024, P=0.05) and a non-significant difference in MDA levels (P=0.129).
Conclusion
Curcumin modulates some of the inflammatory and oxidative stress indices induced by endurance exercise (the FIFA test).
Mojtaba Salehpour, Ali Sarlak, Hadi Samadi,
Volume 21, Issue 26 (12-2023)
Abstract
Introduction: The increase of iron due to aging affects the process of ferroptosis, and the final product of lipid peroxidation, MDA, is the central regulator of ferroptosis. Therefore, the aim of the research was to determine the effect of eight weeks of endurance training on the expression of GPX4 and MDA in the soleus muscle of aged male rats.
Materials and Methods: This study is of an experimental type. 20 healthy rats were divided into two groups of 10 each: training group and control group. The training was done incrementally for eight weeks. 48 hours after the last training session, the soleus muscle of mice was surgically removed and the amount of variables was measured by gene expression and ELISA methods.
Results: There was no significant difference in the level of GPX4 gene expression in the soleus muscle of exercise group rats compared to control group rats (p=0.4079). But the amount of MDA in the training group was significantly reduced compared to the control group (p=0.0028).
Conclusion: According to the findings of the present research, it seems that the effect of endurance training in this research shows a decrease in ferroptosis. The absence of a significant difference in the level of GPX4 gene expression is a sign that endurance training with this intensity does not affect it.
