Gypsophila is the fourth biggest genus of Caryophyllaceae. Presence or absence of indumentum on different parts of the plant is one of the most important diagnostic characters between the species of this genus. The indumentum of 13 taxa of this genus were examined using scanning electron microscopy. All taxa had multicellular and glandular trichomes. Size of trichomes can represent as distinguishing trait between morphologically closed species. Although the most important characters in the delimitation of the sections were morphological, trichomes characters were also supportive. The indumentum was constant among different populations of each species except for two species whose glabrous specimens were described here as new varieties: Gypsophila pilosa var. glabra and Gypsophila polyclada var. leioclada