Nowadays, nanoparticles (NPs) have the great potential application in different industries. Among all NPs, titanium dioxide NPs is the biggest ecotoxicological and ecophysiology concerns due to the increase of anthropogenic input into the aquatic ecosystems. In this study, the effects of titanium dioxide NPs enriched yeast on the growth, survival; digestive enzymes activity and lipid metabolism in Artemia urmiana (AU) and Artemia franciscana (AF) were investigated. The experiment was designed in two treatments (control and enriched yeast with titanium dioxide NPs) and each with four replicates for both Artemia species. At the end of experiment, the results indicated that titanium dioxide nanoparticles did not affect on the Artemia species growth but significantly increased AF survival. No significant difference was observed in AU survival. Also the results showed, NPs significantly decrease AU digestive enzymes activity and reverse pattern was observed for AF. The effect of NPs on the body lipid content was investigated in Artemia species and the results revealed that all the NPs decrease this parameter in AU but did not affect on AF lipid body content. The results obtained in this experiment, suggest that the eco-physiological effects of titanium dioxide NPs different in Artemia urmiana and Artemia franciscana.