Volume 6, Issue 1 (5-2019)                   nbr 2019, 6(1): 20-29 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Saffarzadeh N, Moghimi H. The study of impranil (DLN) polymer biodegradation by fungus Sarocladium kiliense. nbr 2019; 6 (1) :20-29
URL: http://nbr.khu.ac.ir/article-1-2904-en.html
The study of impranil (DLN) polymer biodegradation by fungus Sarocladium kiliense
Neshat Saffarzadeh , Hamid Moghimi *
University of Tehran , hmoghimi@ut.ac.ir
Abstract:   (6504 Views)

Impranil DLN is a class of plastics belonging to the polyurethane family with high application in textile industries. The aim of this study was to evaluate the potential of native strain to degrade impranil DLN. In this study, yeast strains were isolated from different areas and purified in minimal medium containing 1% impranil. Isolate NS-10 was selected as the superior strain capable of degrading impranil and identified through PCR and ITS gene. Esterase, urease and protease assays were carried out for the superior strain. Finally, the biodegradation of impranil was investigated. In total, 40 yeast strains were isolated and isolate NS-10 was selected as a superior strain based on impranil removal assay. NS-10 strain was identified as Sarocladium kiliense with 100% homology. Enzymatic assays showed that the S.kiliense could produce esterase, urease and protease. In addition, it could produce significant clear zones on impranil plates. Degradation rate for impranil was 100% for 10 g/l within 14 days. Finally, S.kiliense was taken to medium containing pure polyurethane film and the capacity of degradation was investigated by the scanning electron microscopy. Our results indicated that S.kiliense is capable of degrading impranil. These results could contribute to a better insight into the mechanism of plastic biodegradation.
 
 

Keywords: bioremediation, enzymatic activity, plastic, polyurethane, scanning electron microscopy
Full-Text [PDF 1041 kb]   (1575 Downloads)    
Type of Study: Original Article | Subject: Microbiology
Received: 2017/07/17 | Revised: 2019/05/5 | Accepted: 2018/09/3 | Published: 2019/04/30 | ePublished: 2019/04/30
References
1. Álvarez-Barragán, J., Domínguez-Malfavón, L., Vargas-Suárez, M., González-Hernández, R., Aguilar-Osorio, G. and Loza-Tavera, H. 2016. Biodegradative activities of selected environmental fungi on a polyester polyurethane varnish and polyether polyurethane foams. - Appl. Environ. Microbiol. 82: 5225-5235. [DOI:10.1128/AEM.01344-16]
2. Biffinger, J.C., Barlow, D.E., Cockrell, A.L., Cusick, K.D., Hervey, W.J., Fitzgerald, L.A., Nadeau, L.J., Hung, C.S., Crookes-Goodson, W.J. and Russell, J.N. 2015. The applicability of Impranil® DLN for gauging the biodegradation of polyurethanes. - Polym. Degrad. Stab. 120: 178-185. [DOI:10.1016/j.polymdegradstab.2015.06.020]
3. Cangemi, J.M., Santos, A.M.D., C Neto, S. and Chierice, G.O. 2008. Biodegradation of polyurethane derived from castor oil. - Polím. 18: 201-206. [DOI:10.1590/S0104-14282008000300004]
4. Cosgrove, L., McGeechan, P.L., Robson, G.D. and Handley, P.S. 2007. Fungal communities associated with degradation of polyester polyurethane in soil. - Appl. Environ. Microbiol. 73: 5817-5824. [DOI:10.1128/AEM.01083-07]
5. Howard, G.T., Norton, W.N. and Burks, T. 2012. Growth of Acinetobacter gerneri P7 on polyurethane and the purification and characterization of a polyurethanase enzyme. - Biodegrad. 23: 561-573. [DOI:10.1007/s10532-011-9533-6]
6. Jones, B.V., Sun, F. and Marchesi, J.R., 2007. Using skimmed milk agar to functionally screen a gut metagenomic library for proteases may lead to false positives. - Lett. Appl. Microbiol. 45: 418-420. [DOI:10.1111/j.1472-765X.2007.02202.x]
7. Khan, S., Nadir, S., Shah, Z.U., Shah, A.A., Karunarathna, S.C., Xu, J., Khan, A., Munir, S. and Hasan, F. 2017. Biodegradation of polyester polyurethane by Aspergillus tubingensis. - Environ. Pollut. 225: 469-480. [DOI:10.1016/j.envpol.2017.03.012]
8. Mahajan, N. and Gupta, P. 2015. New insights into the microbial degradation of polyurethanes. - RSC Adv. 5: 41839-41854. [DOI:10.1039/C5RA04589D]
9. Mukherjee, K., Tribedi, P., Chowdhury, A., Ray, T., Joardar, A., Giri, S. and Sil, A.K. 2011. Isolation of a Pseudomonas aeruginosa strain from soil that can degrade polyurethane diol. - Biodegrad. 22: 377-388. [DOI:10.1007/s10532-010-9409-1]
10. Osman, M., Satti, S.M., Luqman, A., Hasan, F., Shah, Z. and Shah, A.A. 2017. Degradation of Polyester Polyurethane by Aspergillus sp. Strain S45 Isolated from Soil. - J. Polym. Environ. 26: 301-310. [DOI:10.1007/s10924-017-0954-0]
11. Peng, Y.H., Shih, Y.H., Lai, Y.C., Liu, Y.Z., Liu, Y.T. and Lin, N.C. 2014. Degradation of polyurethane by bacterium isolated from soil and assessment of polyurethanolytic activity of a Pseudomonas putida strain. - Environ. Sci. Pollut. Res. 21: 9529-9537. [DOI:10.1007/s11356-014-2647-8]
12. Rowe, L. and Howard, G.T. 2002. Growth of Bacillus subtilis on polyurethane and the purification and characterization of a polyurethanase-lipase enzyme. - Int. Biodeterior. Biodegrad. 50: 33-40. [DOI:10.1016/S0964-8305(02)00047-1]
13. Ruiz, C., Main, T., Hilliard, N.P. and Howard, G.T. 1999. Purification and characterization of two polyurethanase enzymes from Pseudomonas chlororaphis. - Int. Biodeterior. Biodegrad. 43: 43-47. [DOI:10.1016/S0964-8305(98)00067-5]
14. Russell, J.R., Huang, J., Anand, P., Kucera, K., Sandoval, A.G., Dantzler, K.W., Hickman, D., Jee, J., Kimovec, F.M., Koppstein, D. and Marks, D.H. 2011. Biodegradation of polyester polyurethane by endophytic fungi. - Appl. Environ. Microbiol. 77: 6076-6084. [DOI:10.1128/AEM.00521-11]
15. Scott, G. 1990. Photo-biodegradable plastics: their role in the protection of the environment. - Polym. Degrad. Stab. 29: 135-154. [DOI:10.1016/0141-3910(90)90026-4]
16. Shah, A.A., Hasan, F., Akhter, J.I., Hameed, A. and Ahmed, S. 2008. Degradation of polyurethane by novel bacterial consortium isolated from soil. - Ann. Microbiol. 58: 381-386. [DOI:10.1007/BF03175532]
17. Shah, Z., Hasan, F., Krumholz, L., Aktas, D.F. and Shah, A.A. 2013. Degradation of polyester polyurethane by newly isolated Pseudomonas aeruginosa strain MZA-85 and analysis of degradation products by GC-MS. - Int. Biodeterior. Biodegrad. 77: 114-122. [DOI:10.1016/j.ibiod.2012.11.009]
18. Vega, R.E., Main, T. and Howard, G.T. 1999. Cloning and expression in Escherichia coli of a polyurethane-degrading enzyme from Pseudomonas fluorescens. - Int. Biodeterior. Biodegrad. 43: 49-55. [DOI:10.1016/S0964-8305(98)00068-7]
19. White, T.J., Bruns, T., Lee, S.J.W.T. and Taylor, J.W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols. - Academic Press, San Diego, CA. [DOI:10.1016/B978-0-12-372180-8.50042-1]
20. Zafar, U., Nzeram, P., Langarica-Fuentes, A., Houlden, A., Heyworth, A., Saiani, A. and Robson, G.D. 2014. Biodegradation of polyester polyurethane during commercial composting and analysis of associated fungal communities. - Bioresour. Technol. 158: 374-377. [DOI:10.1016/j.biortech.2014.02.077]
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons Licence
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.



© 2024 CC BY-NC 4.0 | Nova Biologica Reperta

Designed & Developed by : Yektaweb