Volume 6, Issue 2 (8-2019)
nbr 2019, 6(2): 217-228 |
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Naeemi T, Fakheri B. Effect of drought stress on MYB gene expression and osmotic regulator levels of five durum wheat genotypes (Triticum turgidum L.). nbr 2019; 6 (2) :217-228
URL: http://nbr.khu.ac.ir/article-1-3028-en.html
URL: http://nbr.khu.ac.ir/article-1-3028-en.html
Abstract: (5646 Views)
Plant growth is greatly influenced by environmental stresses including water deficit, salinity and extreme temperatures. Therefore, the identification of genes, especially regulatory ones whose expression enables plants to adapt to or to tolerate these abiotic stresses, is very essential. MYB proteins, a superfamily of transcription factors, play regulatory roles in developmental processes and in defense responses in plants. Members are characterized by a structurally conserved DNA-binding domain, i.e., the MYB domain. Thus, a factorial experiment in a randomized complete block design with three replications was carried out to investigate the effect of different levels of drought stress on the relative expression of TaMYB73 transcription factor gene using Real Time PCR method at Biotechnology Research Institute of Zabol University. The experimental treatments included durum wheat genotypes (Shabrang, Behrang, Karkheh, Aria and Dena) and drought levels (5, 10, 15, 20 and 25% of field capacity). Seedlings of genotypes
were cultured in pots and drought stress was exerted after 45 days (four to five leaves). Data analysis was performed using Ratio =2 -ΔΔCT and SAS software version 9.1. The results of the 2-way ANOVA for the effect of genotype, drought stress and cross-effects of drought stress on the relative expression of TaMYB73 gene and the amount of osmotic regulators (Proline and Carbohydrate) at different stress levels (20, 15, and 5% of field capacity) were significant in comparison with the normal condition (25% of field capacity) at 1% probability level. With the increase of drought stress level from 5 to 20% of crop capacity, relative to the normal level (25% crop capacity), the relative expression of TaMYB73 gene and osmotic regulators of proline and carbohydrates increased in Beharang, Karkheh and Dena genotypes. Therefore, Karkheh and Dena genotypes showed greater resistance to drought stress among the 5 genotypes studied.
were cultured in pots and drought stress was exerted after 45 days (four to five leaves). Data analysis was performed using Ratio =2 -ΔΔCT and SAS software version 9.1. The results of the 2-way ANOVA for the effect of genotype, drought stress and cross-effects of drought stress on the relative expression of TaMYB73 gene and the amount of osmotic regulators (Proline and Carbohydrate) at different stress levels (20, 15, and 5% of field capacity) were significant in comparison with the normal condition (25% of field capacity) at 1% probability level. With the increase of drought stress level from 5 to 20% of crop capacity, relative to the normal level (25% crop capacity), the relative expression of TaMYB73 gene and osmotic regulators of proline and carbohydrates increased in Beharang, Karkheh and Dena genotypes. Therefore, Karkheh and Dena genotypes showed greater resistance to drought stress among the 5 genotypes studied.
Keywords: carbohydrate, drought stress, proline, Real Time PCR method, seedling stage, transcription factor
Type of Study: Original Article |
Subject:
Genetics
Received: 2017/12/16 | Revised: 2019/09/3 | Accepted: 2018/12/1 | Published: 2019/07/30 | ePublished: 2019/07/30
Received: 2017/12/16 | Revised: 2019/09/3 | Accepted: 2018/12/1 | Published: 2019/07/30 | ePublished: 2019/07/30
References
1. Amiri Deh Ahmadi, S.R., Parsa, M. and Ganjeali, A. 2010. Effects of drought stress on morphological characteristics and yield components in different phenological stages of chickpea (Cicer arietinum L.) greenhouse conditions. - JAR. 8: 166-157.
2. Bates, S., Waldern, R.P. and Teare, E.D. 1973. Rapide determination of free proline for water stress studies - Plant Soil 39: 205-207. [DOI:10.1007/BF00018060]
3. Boon Jung, H. and Fukai, S. 1996. Effects of soil water deficit at different growth stages on rice growth and yield under upland condition. - Field Crops Res. 48: 47- 55. [DOI:10.1016/0378-4290(96)00039-1]
4. Briggle, L.W. and Curtis, B.C. 1987. A, SSSA, Madison, WI, USA. pp: 1-32.
5. Cai, H., Tian, S., Dong, H. and Guo, C. 2015. Pleiotropic effects of TaMYB3R1 on plant development and response to osmotic stress in transgenic Arabidopsis. - Gene 558: 227-234. [DOI:10.1016/j.gene.2014.12.066]
6. Cushman, J. C. and Bohnert, H. J. 2000. Genomic approaches to plant stress tolerance. - Curr. Opin. Plant Biol. 3: 117-124. [DOI:10.1016/S1369-5266(99)00052-7]
7. Dai, X., Xu, Y., Ma, Q., Xu, W., Wang, T., Xue, Y. and Chong, K. 2007. Overexpression of an R1R2R3MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in ransgenic Arabidopsis. - J. Plant Physio. 143: 17391751. [DOI:10.1104/pp.106.094532]
8. Ding Zhenhua., Li Shiming., An Xueli., Liu Xin., Qin Huanju., Wang Daowen. 2009. Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana. - J. Genet. Genomics 36: 17-29. [DOI:10.1016/S1673-8527(09)60003-5]
9. Dubos, C., Stracke, R., Grotewold, E., Weisshaar, B., Martin, C. and Lepiniec, L. 2010. MYB transcription factors in Arabidopsis. - J. Trend. Plant Sci. 15: 573-581. [DOI:10.1016/j.tplants.2010.06.005]
10. Esfandiari, E., Javadi, A., Shokrpour, M. and Shekari, F. 2011. The effect of salt stress on the antioxidant defense mechanisms on wheat seedling. - Fresen. Environ. Bull. 20: 2021-2036.
11. Fowler, S. and Thomashow, M.F. 2002. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. - Plant Cell 14: 1675-1690. [DOI:10.1105/tpc.003483]
12. Galle, A., Csiszár, J., Tari, I. and Erdei, L. 2002. Changes in water and chlorophyll fluorescence parameters under osmotic stress in wheat cultivars. - Acta Biol Szeged. 46: 85-86.
13. Gharbi, A., Rashidin, A.S. and Tarynzhad. Chlbyyany, Q. 2013. Salinity and drought tolerance of durum wheat lines under greenhouse conditions. - J. Crop Ecophysio 4: 393-410.
14. Ghorbanli, M. and Niakan, M. 2005. Studing the effect of drought stress on, protein, proline, phenolic compounds and nitrate reductase enzyme activity in Gorgan. - JSTE 5: 537-550.
15. Hoque, A. and Arima, S. 2002. Overcoming phenolic accumulation during callus induction and in vitro organogenesis in water chestnut (Trapa japonica Flerov). - In Vitro Cell Dev. Biol. Plant 38: 342-350. [DOI:10.1079/IVP2002305]
16. Irrigoyen, J.H., Emerich, D.W. and Sanchez Diaz, M. 1992. Water stress induced changes in concentration of proline and total soluble sugars in nodulated alfalfa plant. - Physiol. Pant 84: 55-66. [DOI:10.1034/j.1399-3054.1992.840109.x]
17. Javadi, S.M., Shabr, Z.S., Pour Abed, A. and Ghadiri, Sh. 2016. Reconstruction and analysis of drought tolerance gene networks in Hordeum vulgare leaf. - The First International Conference and the 9th National Biotechnology Conference of the Islamic Republic of Iran. May 3rd to 5th. Tehran.
18. Johari-Pireivatlou, M. 2010. Effect of soil water stress on yield and proline content of four wheat lines. - Afr. J. Biotechnol. 9: 36-40.
19. Lamas, A., Ullrich, C.I. and Sanz, A. 2000. Cadmium effects on transmembrance electrical potential difference, respiration and membrane permeability of rice (Oryza sativa) roots. - Plant Soil 219: 21-28 [DOI:10.1023/A:1004753521646]
20. Maali-Amiri, R.I.V., Goldenkova-Pavlova, V., Pchelkin, V.D., Tsydendambaev, A.G., Vereshchagin, A.N., Deryabin, T.I., Trunova, D.A. and Los. Nosov, A.M. 2007. Lipid fatty acid composition of potato plants transformed with the Δ12-desaturase gene from cyanobacterium. - Russ. J. Plant Physl. 54: 678-685. [DOI:10.1134/S1021443707050056]
21. Majidi Harvan, A. 1993. Physiological mechanisms of resistance to environmental constraints. - Congress abstracts Crop Iran, Tehran University, 6-9 Sep. pp: 133-134.
22. Mamnoei, E. and Seyed Sharifi, R. 2010. Study the effects of water deficit on chlorophyll fluorescence indices and the amount of proline in six barley genotypes and its relation with canopy temperature and yield. - J. Plant Biol. 2: 51-62.
23. Michalak, p. 2006. RNA world - the dark matter of evolutionary genomics. - ESEB. 19: 1768-1774. [DOI:10.1111/j.1420-9101.2006.01141.x]
24. Mohammadkhani, N. and Heidari, R. 2008. Drought-induced accumulation of soluble sugars and proline in two maize varieties. - WASJ 3: 448-453.
25. Naghavi, M.R. and Mardi, M. 2010. Characterization of genetic variation among accessions of Aegilops tauschii. - APJMBB 18: 93-96.
26. Nakashima, K. 2000. Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration-and high-salinity-responsive gene expression. - Plant Mol. Boil. 42: 657-665.
27. Paz-Ares J, Ghosal D, Wienand U, Peterson PA, Saedler H. 1987. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. - EMBO 6: 3553-3558 [DOI:10.1002/j.1460-2075.1987.tb02684.x]
28. Rahaei, M. Xue, GP., Naghavi, MR., Alizadeh, H., Schenk, PM. 2010. A MYB gene from wheat (Triticum aestivum L.) is up-regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes. - Plant Cell Rep. 29: 835-44. [DOI:10.1007/s00299-010-0868-y]
29. Riechmann, J.L. and Ratcliffe, O.J. 2000. A genomic perspective on plant transcription factors. - Curr. Opin. Plant Biol. 3: 423-434. [DOI:10.1016/S1369-5266(00)00107-2]
30. Sarmadnia, Gh. 1993. The Importance of environmental tensions in agriculture. - Key articles of the 1st Iranian Congress of Plant Breeding, University of Tehran. pp: 157-169.
31. Tabaraki, H., Fahmideh, L. and Fooladvan, Z. 2017. Study of MYB gene expression under drought stress in some bread wheat cultivars. - EJBGE 6: 95-104.
32. Tajbakhsh, D. and Pourmirza, A.S. 2003. Cultivation of crops. - Jihad Urmia University Publications 68 p.
33. Tavakoli Hassanluklo, H., Ebadi, A. and Jahanbakhsh, S. 2014. Investigation of some mechanisms of water stress tolerance in bread wheat genotypes. - J. Creal. Res. 4: .13-25.
34. Turkan, I. 2011. Plant responses to drought and salinity stress, development in a post-genomic era. - Adv. Bot. Res. 593 pp.
35. Umezawa, T. Fujita, M., Fujita, Y., Yamaguchi-Shinozaki, K. and Shinozaki, K. 2006. Engineering drought tolerance in plants: discovering and tailoring genes to unlock the future. - Curr. Opin. Plant Biol. 17: 113-122. [DOI:10.1016/j.copbio.2006.02.002]
36. Vinocur, B. and Altman, A. 2005. Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. - J. Curr. Opin. Biotech. 16: 123-132. [DOI:10.1016/j.copbio.2005.02.001]
37. Wang, W., Vinocur, B. and Altman, A. 2003. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering fortress tolerance. - J. Planta 218: 1-14. [DOI:10.1007/s00425-003-1105-5]
38. Yamaguchi-Shinozaki, K. and Shinozaki, K. 2005. Organization of cis-acting regulatory elements in osmotic- and cold-stress responsive promoters. - Trends Plant Sci. 10: 88-94. [DOI:10.1016/j.tplants.2004.12.012]
39. Zarei, L., Farshadfar, E., Haghparast, R., Rajabi, R. and Mohammadi Sarab Badieh, M. 2015. Evaluation of physiological, phenological and morphological traits associated with drought tolerance in bread wheat. - J. Creal. Res. 5: 327-340.
40. Zhang, L., Liu, G., Zhao, G., Xia, C., Jia, J., Liu, X. and Kong, X. 2014. Characterization of a wheat R2R3-MYB transcription factor gene, TaMYB19, involved in enhanced abiotic stresses in Arabidopsis. - Plant Cell Physiol. 55: 1802-1812. [DOI:10.1093/pcp/pcu109]
41. Zhu, J.K. 2002. Salt and drought stress signal transduction in plants. - Annu. Rev. Plant Biol. 53: 247-273. [DOI:10.1146/annurev.arplant.53.091401.143329]
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