Volume 6, Issue 2 (8-2019)                   nbr 2019, 6(2): 198-205 | Back to browse issues page

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Sagharyan M, Ganjeali A, Cheniany M. Investigating the effect of antioxidant compounds and various concentrations of BAP and NAA on the improvement of in vitro stem and root formation of Nepeta binaloudensis Jamzad . nbr 2019; 6 (2) :198-205
URL: http://nbr.khu.ac.ir/article-1-3138-en.html
Investigating the effect of antioxidant compounds and various concentrations of BAP and NAA on the improvement of in vitro stem and root formation of Nepeta binaloudensis Jamzad
Mostafa Sagharyan , Ali Ganjeali * , Monireh Cheniany
Ferdowsi University of Mashhad , ganjeali@um.ac.ir
Abstract:   (5246 Views)
Nepeta binaloudensis Jamzad is a medicinal plant endemic to Iran. It is an endangered plant due to habitat destruction and intensive harvest. We investigated the effect of antioxidants and different concentrations of BAP and NAA on in vitro stem and root formation of N. binaloudensis. Stem explants were cultured in ½ MS medium supplemented with BAP (0.5 mg/L) and different concentrations of ascorbic acid and reduced glutathione. The effect of different concentrations of BAP on the regeneration of this plant was then evaluated. Moreover, root formation of regenerated stems was investigated in the ½ MS medium supplemented with different concentrations of NAA. The results showed that the combination of antioxidants in ½ MS medium supplemented with BAP (0.5 mg/L) had a significant effect on regeneration in vitro culture. The reduced-glutathione (2 μM/ L) in comparison with other antioxidant treatments increased the stem regeneration in explants. The levels of BAP hormone (1 and 1.5 mg/L) had a significant (p-value<0.05) effect on the stem regeneration rate and the number of produced branches. The NAA (2 mg/L) increased root formation and root height average. We recommend the use of these treatments for in vitro propagation of this endangered plant.
 
Keywords: culture medium, growth optimization, in vitro culture, Lamiaceae, shoot proliferation
Full-Text [PDF 718 kb]   (1460 Downloads)    
Type of Study: Original Article | Subject: Plant Biology
Received: 2018/06/18 | Revised: 2019/08/31 | Accepted: 2018/08/2 | Published: 2019/07/30 | ePublished: 2019/07/30
References
1. Aicha, N. and Abdelmalek, E. 2014. In vitro regeneration and clonal multiplication of Thymus bleicherianus Pomel, a rare and threatened medicinal and aromatic in Morocco. - J. Med. Aromat. Plants 3: 145. [DOI:10.4172/2167-0412.1000145]
2. Aliyo, O.M. 2005. Application of tissue culture to cashew (Anacardium occidentale) breeding: an appraisal. - AJB 4: 1485-1489.
3. Andrade, L. and Basso, R. 2005. Micropropagation of Lavandula dentata from axillary buds of field-grown adult plants. - Biol. Plantarum 3: 439-442. [DOI:10.1007/s10535-005-0024-7]
4. Arikat, N.A, Javad F.M., Karam, N.S. and Shilbi, R.A. 2004. Micropropagatin and accumulation of essential oil in wild sage (Salvia fruticosa Mill.). - Sci. Hortic. 100: 193-202. [DOI:10.1016/j.scienta.2003.07.006]
5. Arnon, D. and Hoagland, D. 1940. Crop production in artificial culture solutions in soils with special reference to factors influencing yields and absorption of inorganic nutrients. - J. Soil Sci. 50: 463-485.
6. Daniel, A., Kalidass, C. and Mohan, V.R. 2010. In vitro multiple shoot induction through axillary bud of Ocimum basilicum L. an important medicinal plant. - IJBT 5: 24-28.
7. De Pinto, M.C., Tommasi, F. and De Gara, L. 2000. Enzymes of the ascorbate biosynthesis and ascorbate-glutathione cycle in cultured cells of tobacco Bright Yellow 2. - Plant Physiol. Biochem. 38: 541-550. [DOI:10.1016/S0981-9428(00)00773-7]
8. Dibax, R., Eisfeld, C., Chuquel, F., Koehler, H. and Quoirin, M. 2005. Plant regeneration from cotyledonary explants of Eucalyptus camalduensis. - Sci. Agricola. 62: 406-412. [DOI:10.1590/S0103-90162005000400016]
9. Dutta Gupta, S. and Datta, S. 2003. Antioxidant enzyme activities during in vitro morphogenesis of gladiolus and the effect of application of antioxidants on plant regeneration. - Biol. Plant 47: 179-183. [DOI:10.1023/B:BIOP.0000022248.62869.c7]
10. Ewa, S., and Wysokinska, H. 2004. In vitro regeneration of Salvia nemerosa L. from shoot tips and leaf explants. - In vitro Cell Dev. Biol. Plant. 40: 596-602. [DOI:10.1079/IVP2004580]
11. Ghahreman, A. 1999. Biodiversity of Plants in Iran. Tehran University, Iran Synder. R.L. (1985). Hand calculating degree-days. - J. Agri. Meteoy. 35: 353-358. [DOI:10.1016/0168-1923(85)90095-4]
12. Ghannadi, A., Aghazari, F., Mehrabani, M., Mohagheg-hzadeh, A. and Mehregan, I. 2003. Quantity and comp-osition of the SDE prepared essential oil of Nepeta macrosiphon Bioss. - Iran. J. Pharm. Res. 2: 5-103.
13. Ghanti, K., Kaviraj, C.P., Venugopal, R.P., Jabeen, F.T.Z. and Rao, S. 2003. Rapid regeneration of Mentha piperita L. of shoot tip and nodal explant. - IBJT 5: 594-598.
14. Jamzad, Z. 2012. Lamiaceae. - In: Assadi, M. et al. (eds.): Flora of Iran, No. 76. - RIFR, Tehran. 1066 pp.
15. Jose, M.C.S., Romero, L. and Janeiro, L.V. 2012. Effect of Indole-3-Butyric Acid on root formation in Alnus glutinosa. - Silva Fenn. 46: 643-654. [DOI:10.14214/sf.916]
16. Joy, R.W., Patel, K.R. and Thorpe, T.A. 1988. Ascorbic acid enhancement of organogenesis in tobacco callus. - PCTOC 13: 219-22. [DOI:10.1007/BF00043670]
17. Kariyana, K. and Nisyawati, N. 2013. Effect of ascorbic acid, activated carbon and light duration on explant
18. browning of banana cultivar barangan (Musa acuminata L.) in vitro culture. - IJRRAS 16: 118-123.
19. Karuppusamy, S., Kiranmai, C., Aruna, V. and Pullaiah, T. 2006. Micropropagation of Vanasushava pedata. An endangered medicinal plant of South India. - BAPTCB 16: 85-94. [DOI:10.3329/ptcb.v16i2.1109]
20. Mederos Molina, S. 2006. Micropropagation of Salvia broussonetii Benth. a medicinal plant species. - BAPTCB 16: 19-23. [DOI:10.3329/ptcb.v16i1.1101]
21. Modarres, M., Lahouti, M., Gangeali, A. and Asili, J. 2012 Study of micropropagation of Salvia lerifolia Benth. using shoot tip. - J. Plant Biol. 4: 89-100.
22. Murashige, T., and Skoog, F.A. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Plant Physiol. 15: 473-479. [DOI:10.1111/j.1399-3054.1962.tb08052.x]
23. Nadjafi-Pour, N., Valizadeh, M., Mostafavi, E. and Maassomi, A.A. 2016. Micro propagation study of Nepeta binaloudensis Jamzad in vitro culture. International conference on new horizon of biology in agricultural Science. Tehran, Society of Modern Science and Technology.
24. Nadjafi, F., Koocheki, A., Honermeier, B. and Asili, J. 2009. Autecology, Ethnomedicinal and phytochemical studies of Nepeta binaloudensis Jamzad a highly endangered medicinal plant of Iran. - J. Essent. Oil Bear Plant 12: 37-41. [DOI:10.1080/0972060X.2009.10643699]
25. Nomura, K., Matsumoto, S., Masuda, K. and Inoue, M. 1998. Reduced glutathione promotes callus growth and shoot development in a shoot tip culture of apple stock. - Plant Cell Rep. 17: 597-600. [DOI:10.1007/s002990050449]
26. Pei, S. 2001. Ethnobotanical approaches of traditional medicine studies: some experiences from Asia. - Pharm. Biol. 39: 74-79. [DOI:10.1076/phbi.39.7.74.5869]
27. Pellny, T.K., Locato, V., Vivancos, P.D., Markovic, J., De Gara, L., Pallardó, F.V. and Foyer, C.H. 2009. Pyridine nucleotide cycling and control of intracellular redox state in relation to poly (ADPribose) polymerase activity and nuclear localization of glutathione during exponential growth of Arabidopsis cells in culture. - Mol. Plant 2: 442-456. [DOI:10.1093/mp/ssp008]
28. Rout, G.R. 2000. In vitro manipulation and propagation of medicinaplants. - Biotechnol. Adv. 18: 91-120. [DOI:10.1016/S0734-9750(99)00026-9]
29. Rustaiyan, A. and Nadji, K. 1999. Composition of the essential oils of Nepeta isphanica Boiss and Nepeta binaloudensis Jamzad from Iran. - Flavour Fragr. J. 35-37. https://doi.org/10.1002/(SICI)1099-1026(199901/02)14:1<35::AID-FFJ776>3.0.CO;2-N [DOI:10.1002/(SICI)1099-1026(199901/02)14:13.0.CO;2-N]
30. Skala, A. and Wysokinska, A. 2006. In vitro Regeneration of (Salvia nemorosa L.) from shoot tips and leaf explants. - In Vitro Cell Dev. Biol. Plant 1: 90-151.
31. Sulistiarini, D.L. 1999. Ocimum gratissimum L. In: A. P. L. Oyen and D. X. Nguyen, Eds. Plant Resources of South- East Asia No. 19: Essential Oils Plants. Prosea Foundation, Bogor. 140-142.??
32. Tawfik, A. A., and Mohamed M.F. 2007. Regeneration of Salvia officinalis L. via induction of meristematic callus. - In Vitro Cell Devel. Biol. Plant 43: 21-27. [DOI:10.1007/s11627-006-9002-9]
33. Titov, S., Bhowmik, S., Mandal, A., Alam, M. and Nasir, A. 2006. Control of phenolic compound secretion and effect of growth regulators for organ formation from Musa spp. cv. Kanthali floral bud explants. - Am. J. Biochem. Biotech. 2: 97-104. [DOI:10.3844/ajbbsp.2006.97.104]
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