دوره 6، شماره 3 - ( 7-1398 )                   جلد 6 شماره 3 صفحات 291-284 | برگشت به فهرست نسخه ها


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Cucho H, López Y, Caldeira C, Valverde A, Ordóñez C, Soler C. Comparison of three different staining methods for the morphometric characterization of Alpaca (Vicugna pacos) sperm, using ISAS® CASA-Morph system. NBR 2019; 6 (3) :284-291
URL: http://nbr.khu.ac.ir/article-1-3198-fa.html
کوچو هرنان، لوپز یولوی، کالدیرا کارینا، والورده آنتونی، اوردونز سزار، سولر کارلس. مقایسه سه روش رنگ آمیزی مختلف برای توصیف مورفومتریک اسپرم آلپاکا (Vicugna pacos) با استفاده از سیستم ISAS® CASA-Morph. یافته‌ های نوین در علوم زیستی. 1398; 6 (3) :284-291

URL: http://nbr.khu.ac.ir/article-1-3198-fa.html


دانشگاه ملی سن آنتونیو آباد دل کوسکو
چکیده:   (7174 مشاهده)
مورفومتری اسپرم بخشی از آنالیز مایع منی مبتنی بر فناوری CASA است و نقش مهمی در پیش­ بینی باروری مردان نشان داده است. این آنالیز برپایه استفاده از تكنيك هاي مختلف رنگ ­آمیزی است، اگرچه نشان داده شده است که رنگ ­آمیزی می تواند تغییرات چشمگیری در مورفومتری سلول در گونه­ های مختلف ایجاد کند. هدف از این مطالعه، بررسی تفاوتهای موجود در مورفومتري اسپرم درآلپاکا بود كه با استفاده از تكنيك ­هاي مختلف رنگ ­آمیزی انجام شد. یک انزال ازهریک از پنج آلپاکای بالغ مورد استفاده قرار گرفت. نمونه­ ها با استفاده از روش جراحی مجرای دفران جمعآوری شدند. سه روش رنگ­ آمیزی (یعنیHemacolor ، Harri’s Henatoxylin و Diff-Quik) مورد بررسی قرار گرفت. تجزیه و تحلیل مورفومتریک با استفاده از سیستم ISAS® v1 CASA-Morph، با بزرگنمایی 100x و میکروسکوپ زمینه روشن و یک دوربین فیلمبرداری دیجیتالی با وضوح نهایی 08/0 میکرومتر بر پیکسل انجام شد. تقریباً تعداد 200 سلول به صورت تصادفی از هر نمونه انتخاب شد وانتخاب تکنیک رنگ ­آمیزی با توجه به اهمیت پارامترهای اندازه سر اسپرم وبخش میانی انتخاب شد. بین تکنیک ­های به کار رفته تقریباً کلیه پارامترها در سطوح متفاوت تفاوت­ هایی را از خود نشان دادند. به طور کلی، سلول­ هایی که سر بزرگ داشتند با تکنیک Harri’s Henatoxylin و آنهایی که دارای سر کوچک بودند با Diff-Quik رنگ آمیزی شدند. پارامترهای تفکیک کننده برای تمایز بهتر بین حیوانات، عرض سر، سطح و درصد آکروزوم بود. در نتیجه، مانند سایر گونه ها، نتایج مورفومتری اسپرم آلپاکا نیز به تکنیک رنگ ­آمیزی به کاررفته، حساس بود. این دلالت بر لزوم ذکر واضح و دقیق تکنیک رنگ ­آمیزی استفاده شده در هر مورد برای انجام مقایسه بین مطالعات مختلف روی گونه­ های مشابه دارد. هیچ یک از تکنیک ­های به کار گرفته شده برتر از سایرین نبود. علاوه بر این، روشی که برای به دست آوردن نمونه­ ها استفاده شد، سودمندی و سادگی آن را برای نمونه برداری­ های مکرر نشان داد.
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نوع مطالعه: مقاله پژوهشی | موضوع مقاله: علوم جانوری
دریافت: 1397/8/9 | ویرایش نهایی: 1398/8/1 | پذیرش: 1397/11/10 | انتشار: 1398/7/27 | انتشار الکترونیک: 1398/7/27

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