Volume 7, Issue 1 (4-2020)
nbr 2020, 7(1): 76-84 |
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Mohajel Kazemi E, Pazhohandeh M, Jonoubi P, Kazemian M. The optimization of gene transfer to tomato and the study of expression possibility of salt-tolerance gene (SOS3). nbr 2020; 7 (1) :76-84
URL: http://nbr.khu.ac.ir/article-1-3209-en.html
URL: http://nbr.khu.ac.ir/article-1-3209-en.html
Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Iran , e.mohajelkazemi@tabrizu.ac.ir
Abstract: (4504 Views)
One of the main strategies to improve plant tolerance is the expression of stress-induced genes, which play a significant role in the ionic balance of plants. SOS3 is one of the important components of SOS-regulated ionic homeostasis pathway. Therefore, the expression of this gene could be an important step towards producing salt-resistant plants. In this work, we have transformed tomato (Solanum lycopersicum) by Agrobacterium (GV3101 and LBA4404) containing plasmids with SOS3 genes. The maximum regeneration rate was determined in cotyledons of CH genotype. The simultaneous use of cotyledons and hypocotyls in the culture medium had the best outcome. In addition, the best time was found to be one day after inoculation. Also, the best transgenic variety was detected for Agrobacterium GV3101, which can be attributed to the interaction between the genus Agrobacterium and the tomato variety. Transgenic plants were transferred to a culture medium containing sequestrene, which caused the acceleration of the seedling growth in particular. The presence of the SOS3 in the transgenic plants was verified by PCR and RT-PCR methods.
Type of Study: Original Article |
Subject:
Plant Biology
Received: 2018/11/21 | Revised: 2020/07/15 | Accepted: 2019/03/10 | Published: 2020/03/31 | ePublished: 2020/03/31
Received: 2018/11/21 | Revised: 2020/07/15 | Accepted: 2019/03/10 | Published: 2020/03/31 | ePublished: 2020/03/31
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