Determination and quantification of two regulatory phytohormones extracted from Sargassum muticum and Gracilaria corticata seaweeds in the south of Iran

Saebmehr, Hadigheh; Rafiee, Farnaz; Givianrad, Mohammad Hadi; Mostafavi, Golaleh

doi:10.52547/nbr.9.2.115

Volume 9, Issue 2 (9-2022) nbr 2022, 9(2): 115-123 | Back to browse issues page

‎ 10.52547/nbr.9.2.115

‎ 20.1001.1.24236330.2022.9.2.3.5

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Saebmehr H, Rafiee F, Givianrad M H, Mostafavi G. Determination and quantification of two regulatory phytohormones extracted from Sargassum muticum and Gracilaria corticata seaweeds in the south of Iran. nbr 2022; 9 (2) :115-123
URL: http://nbr.khu.ac.ir/article-1-3528-en.html

Determination and quantification of two regulatory phytohormones extracted from Sargassum muticum and Gracilaria corticata seaweeds in the south of Iran

Hadigheh Saebmehr

, Farnaz Rafiee

, Mohammad Hadi Givianrad

, Golaleh Mostafavi ^*

Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran , golaleh.m@gmail.com

Abstract: (2181 Views)

In the present study, two regulatory phytohormones, abscisic acid and auxin, were extracted from two different macroalgae, Sargassum muticum and Gracilaria corticata, for the first time. Sampling was performed each month with three replicates, from Bushehr Province shores located in the north region of Persian Gulf for six alternative months. The alteration of seaweeds biomass and the probable impacts of some environmental factors were measured. The extracted phytohormones were isolated employing HPLC method and identified by injection of standards. The highest amount of ABA phytohormone in the two species, occurred in November. Also, the highest amount of Auxin in Sargassum muticum was reported in May, while the highest amount of this hormone in Gracilaria corticata was observed in January. In January, the maximum mean biomass in Sargassum muticum was 679gr/m², while the minimum mean of 20.66gr/m² was observed in September. Moreover, the highest average biomass in Gracilaria corticata was 423/33gr/m² in March and the lowest mean of 158g/m² was reported in November. ANOVA and Chi-squared test results showed a significant difference in all samples. These phytohormones could be applied in preparing algae liquid fertilizer in future studies.

Keywords: algal biomass, abscisic acid, auxin, hormone extraction, macroalgae

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