Ms Mohammadreza Pourmohammad, Dr Jina Khayatzadeh, Ms Vahid Hanafi, Dr Mehrdad Iranshahi,
Volume 11, Issue 3 (12-2024)
Abstract
Background: Nowadays, due to the increasing growth of production and consumption of nanoparticles, problems such as their entry into fersh waters and aquatic poisoning have arisen. Iron oxide nanoparticles are among the most widely used in this field. Alfalfa (Medicago sativa), a flowering plant belonging to the legume family, contains high amounts of protein, calcium, various vitamins and antioxidant effects. The aim of this research was to investigate the combined effect of iron oxide nanoparticles (Fe2O3)and alfalfa plant on growth parameters and pathology of digestive tract, liver and kidney of koi fish (an example of carp fish with a variety of colors and eye-catching effect).
Materials and Methods: In this experimental research, the number of 60 koi fish larvae were randomly divided into 6 groups including the control group and 5 experimental groups. The effect of feeding koi fish larvae with biomar combined with 10% and 20% alfalfa and being exposed to iron oxide nanoparticles with a concentration of 100 mg/l for 14 days in 5 treatment groups was investigated in comparison with the control group. The collected data were analyzed by Excel and SPSS 20 statistical software.
