Massoud Ranjbar, Azam Pakatchi, Tayebeh Radjabian,
Volume 1, Issue 2 (12-2014)
Abstract
In this research, plant morphology and pollen micromorphology of 14 populations of 3 species belonging to two sections Plethiosphace and Hemisphace of Salvia genus in Iran were studied. In the morphological study 25 quantitative and qualitative characters were investigated and the results clearly separated the species into two groups. The pollen grains from the herbarium specimens were prepared by acetolysis method and then 4 quantitative characters were examined by light microscopy. Data was processed by principal component analysis method. Results indicated that although the pollen grains in the section were comparatively homogenous and all of them were 6-colpate with reticulate ornamentation, a considerable variation in the quantitative characters studied turned out to exist. However among the characters studied, equatorial and polar diameters of pollen grains were the most significant and based on these characters the members of these sections can be divided into two groups.
Azra Saboora, Maryam Amiri Rad, Ezat Asgarani, Tayebeh Radjabian,
Volume 5, Issue 4 (12-2018)
Abstract
DNA extraction from plant tissues often causes most problems. For example, unsuccessful removal secondary metabolites during extraction, such as phenolic compounds in aromatic and medicinal plants, cause to some mistakes in result of molecular experiments by using of the extracted DNA. Achillea wilhelmsii is a medicinal plant belong to Asteraceae family and native to Iran, there is little information about genomic data in this plant. Therefore, optimizing of the DNA extraction methods for obtaining suitable quality and quantity yield is necessary. In this study, two traditional DNA extraction methods (using fresh and herbarium leaf samples) and commercial DNA kit MAGNANTM in Yarrow have been compared. Results showed that DNA extracted from fresh leaves of yarrow according to Khanuja et al. (1999) was better than the other methods which mentioned in this research, because of the increased amount of extracted DNA and reduced harmful compounds such as RNA, polysaccharides, protein and secondary metabolites. To evaluate the quality of extracted DNA from herbarium specimens showed that, in spite of the high yielding DNA (10-50 fold ratio to the other methods), quality of this extract was low on agarose gel because of smear and broken down the molecules. It can be considered as a limitation of the extract in molecular experiments. Procedure of MAGNANTM DNA kit was appropriate for reducing time and cost of the extraction as well as low contamination to protein and RNA, but the process needs some modifications for yarrow to increase the amount of extracted DNA.
