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Alireza Iranbakhsh, Alireza Ghaderi,
Volume 7, Issue 2 (7-2020)
Abstract

The aim of this research was to study the effect of iron oxide nanoparticles (FeO NPs) on the growth, differentiation, anatomy, and physiology of pepper (Capsicum annuum L.) on the basis of a completely randomized design in vitro condition. Seedlings were cultured in MS medium containing four concentrations of FeO NPs (0, 1, 10, and 20 mgl-1). Also, the effect of the different concentrations of FeO NPs on callus formation under two various hormone conditions (0.5mgl-1 2,4D+0.5 mgl-1 BAP or 0.5 mgl-1 BAP+1 mgl-1 Kin) were assessed. The results showed that the application of FeO NPs significantly increased biomass accumulation in both roots and shoots. Moreover, FeO NPs enhanced the concentrations of photosynthesis pigments (chllrophyll a, chlorophyll b, and carotenoids). The presence of FeO NPs in culture medium affected callus formation in a hormone-dependent manner. Different concentration of FeO NPs induced the callus formation under 2, 4-D and BAP treatments. However, it did not significantly increase callus formation under the kinitin and 2,4-D. The findings of this research indicated that the application of FeO NPs at optimized doses may improve plant production, especially in vitro condition.


Ali Ganjeali, Reyhaneh Bahrami Totmaj, Parwaneh Abrishamchi,
Volume 11, Issue 3 (12-2024)
Abstract

The present study aimed to investigate the effect of some bio-elicitors and silver nitrate on the content of secondary metabolites in calli derived from leaf explants of silybum marianum. In this research, the best callus was achieved on MS medium containing KIN, thus this medium was chosen for further experiment. In the second experiment, leaf explants were cultured in a selected hormone-regulated medium. After 8 weeks of callus growth, bio elicitors including yeast Yarrowia lipolytica, fungus Aspergillus niger, and bacterium Pseudomonas putida in combination with silver nitrate as a non-living elicitor were added to the leaf-derived calli. Ten days after treatment, the phenolic compound content, antioxidant capacity, silymarin content, and PAL enzyme activity in the calli were measured. The results indicate that the simultaneous application of Yarrowia yeast and silver nitrate led to an improvement in the content of phenolic derivatives, silymarin, and PAL enzyme activity, and this was significant compared to other elicitors both with silver nitrate and without it. The researchers of this experiment suggest that the combined use of bio-elicitors especially Yarrowia lipolytica yeast, and silver nitrateinduces higher synthesis of medicinal metabolites in calli derived from leaf explants. 

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