Nasrin Esmailnejad, Jalil Khara, Mehrdad Akhgari,
Volume 6, Issue 4 (12-2019)
Abstract
To investigate the effect of herbicide Trifluralin and the role of magnetized water on enzymatic activities and content of proline and growth substances including gibberellin (GA3) and cytokinin (6- benzyl amino purine), an experiment was conducted using squash (Cucurbita pepo L. var. Shiraz Hybrid F1) seedlings. Four levels of trifluralin (0, 5, 15 and 25 ppm) and irrigation by distilled and magnetized water were applied in a completely randomized design in 3 replicates at Urmia University in 2016. Different levels of trifluralin were added to pots 2 days after planting. Seedlings were treated by modified Hoagland solution with diurnal temperature 30:18 °C, relative humidity of 70-80 percent and light period of 16:8 hr, during a 5-week growing period in growth chamber. The effect of magnetized water and trifluralin on ornithine amino-transferase (OAT), proline dehydrogenase (PDH) and content of proline in both shoots and roots as well as GA3 and cytokinin was significant (p>0.01) according to ANOVA. The content of proline and OAT in roots and shoots as well as GA3 and cytokinin increased significantly, although the PDH of roots and shoots decreased under the influence of magnetized water. The content of proline and OAT increased and GA3, cytokinin and the activity of PDH declined by the increase of the levels of trifluralin. Overall, it can be concluded that the growth and biochemical indices of squash plants were improved by magnetized water under toxicity of herbicide trifluralin.
Fereshteh Heidargholinezhad, Yousef Hamidoghli, Valliollah Ghasemiomran, Pouria Biparva,
Volume 9, Issue 4 (12-2022)
Abstract
Centella asiatica, is well known to be a valuable medicinal plant for producing valuable compounds such as asiaticoside, asiatic acid, madcasoside and madcasic acid. The plant is believed to improve memory, lower blood pressure, be a strong antioxidant and anticancer. Therefore, it is important to optimize tissue culture methods in order to facilitate the extraction of medicinal compounds, gene transfer as well as improvement of medicinal properties of the plant. Calli prepared from various medicinal plants can be used to increase the amount of medicinal compounds in the cell suspension culture and gene transfer. The aim of this study was to investigate the effect of different concentrations of two hormones, BAP and NAA, on leaf explant for callus initiation, as one of the important sources of secondary metabolites production. For this purpose, leaf explants were treated with 6 different concentrations of BAP (0, 0.5, 1, 1.5, 2.5, 3.5 mg/L) and 5 different concentrations of NAA (0, 0.25, 0.5, 1, 2 mg/L). The results of this study showed that the best callus was obtained by the combination 1.5 mg/L of BAP and 0.5 mg/L of NAA, resulting in the leaf explants with callus induction of 100%, fresh weight of 1.457 gr and callus diameter of 1.459 cm. The results showed that two hormones of BAP and NAA have synergistic effects on the increase of the quality and quantity of the produced calli.
