Roya Karamian, Fatemeh Ghasemlou,
Volume 1, Issue 2 (12-2014)
Abstract
Saponins are secondary metabolites that are found in many plants and some animals. These compounds are high molecular weight glycosides, consisting of a sugar moiety linked to a triterpene or steroid aglycone. Many saponins have detergency properties and give stable foam in water. The genus Silene L. with more than 700 species is one of the largest genera of the family Caryophyllaceae mainly distributed in northern hemisphere. Saponins are one of the important secondary metabolites in the members of the genus. Quantitative and qualitative study of the saponins in the aerial parts and roots of three Silene species, namely S. ginodioica Ghaz. subsp. Penducularis (Fenzl ex Boiss.) Melzh., S. spergulifolia (Willd.) M. Bieb. and S. swertiifolia Boiss. were carried out by spectrophotometry and TLC methods. In this study, 5 fractions were obtained from each plant part and in both parts of the three species, fraction 1 contains the highest amounts of saponins. On the other hands, root extracts have high amounts of saponins. In addition, thin layer chromatography (TLC) of the extracts revealed some saponins spots with different Rf.
Razieh Sadat Solouki Nezhad, Hanieh Asaadi, Yaser Eshaghi Milasi, Sajjad Yazdansetad,
Volume 6, Issue 1 (5-2019)
Abstract
The production of pigments from bacteria is significant due to the low cost, high yield and ease of extract compared with other sources. Carotenoids are one of the most important pigments with antioxidant properties which are the precursor of vitamin A synthesis and have antibody overproduction ability, anti-tumor activity and inhibitory effect on the cardiovascular disease. The present study aimed to isolate and identify carotenoid-producing bacteria by high-performance liquid chromatography (HPLC) analysis of their carotenoid pigments. Twenty soil samples were collected from different regions of Tehran. After serial dilution each sample was cultured on BHI agar medium and incubated at 37°C. The pigment-producing bacteria were selected for further identification and their pigments were extracted by methanol. The screening was carried out at two levels: i) selection of the strains by visual color inspection, ii) analysis of the pigment extracts by UV-VIS spectroscopy and HPLC. The isolates were identified by phenotypic methods and their 16S rDNA gene was amplified by PCR method and sequenced. Staphylococcus epidermidis, Micrococcus aloeverae, Citricoccus alkalitolerans, Rhodococcus zopfii, Arthrobacter agilis, Dietzia natronolimnaea and Rhodococcus ruber were identified as carotenoid-producing strains. The highest rate of absorption was observed using UV-VIS analysis in Staphylococcus epidermidis and Dietzia natronolimnaea. The comparison of HPLC analysis with the standard β-carotene curve showed that the carotenoids were beta-carotene. Micro-organisms are a potential source in the production of pigments. In this study we introduced two genera of bacteria (Staphylococcus epidermidis and Dietzia natronolimnaea) with carotenoid-producing ability.
