Volume 6, Issue 4 (12-2019)                   nbr 2019, 6(4): 367-373 | Back to browse issues page

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Ghasemian F. The evaluation of sperm chromatin status in men with different infertility factors, and its relationship with ICSI outcomes. nbr 2019; 6 (4) :367-373
URL: http://nbr.khu.ac.ir/article-1-2895-en.html
The evaluation of sperm chromatin status in men with different infertility factors, and its relationship with ICSI outcomes
Fatemeh Ghasemian *
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran , ghasemian.21@gmail.com
Abstract:   (4303 Views)

One of the most important factors related to male fertility is sperm chromatin status. Under Assisted Reproductive Techniques (ART), especially Intracytoplasmic Sperm Injection (ICSI), the natural selection of high quality sperm with intact chromatin is not possible. Therefore, embryo quality and pregnancy rate decreased for damaged DNA of sperms involved in the fertilization of eggs. Thus, this study evaluated the frequency of abnormal sperm chromatin condensation and damaged sperm chromatin in men with different infertility factors (e.g. oligozoospermia, asthenozoospermia, teratozoospermia, oligasthenoteratozoospermia, and normozoospermia) and ICSI outcomes were examined. 195 patients were examined and the sperm chromatin status was evaluated using aniline blue and toluidine blue staining. Fertilization, zygote and embryo quality, chemical pregnancy and abortion rates were calculated, too. The results showed that in the oligoasthenoteratozzoospermia samples, the damaged chromatin percentage was higher than that in normozoospermia ones. The fertilization rate, embryo quality, and pregnancy rate significantly decreased in this group. Also, a higher abortion rate was observed in this group (P<0.05). In conclusion, the frequency of damaged chromatin was observed to be different in different male infertility factors, which could influence the ICSI outcomes. Therefore, the evaluation of sperm chromatin status before cycle initiation was found to promote ICSI outcomes.
 

Keywords: assisted reproductive techniques, embryo, fertilization, pregnancy, male factor infertility
Full-Text [PDF 1195 kb]   (1932 Downloads)    
Type of Study: Original Article | Subject: Animal Biology
Received: 2017/07/6 | Revised: 2020/02/24 | Accepted: 2019/04/22 | Published: 2020/01/8 | ePublished: 2020/01/8
References
1. Aboulghar, M.A., Mansour, R.T., Serour, G.I., Fahmy, I., Kamal, A., Tawab, N.A. and Amin, Y.M. 1997. Fertilization and pregnancy rates after intracytoplasmic sperm injection using ejaculate semen and surgically retrieved sperm. - Fertil. Steril. 68: 108-111. [DOI:10.1016/S0015-0282(97)81484-7]
2. Aitken, R.J. and De Iuliis, G.N. 2007. Origins and consequences of DNA damage in male germ cells. - Reprod. Biomed. Online. 14: 727-733. [DOI:10.1016/S1472-6483(10)60676-1]
3. Avendaño, C., Franchi, A., Duran, H. and Oehninger, S. 2010. DNA fragmentation of normal spermatozoa negatively impacts embryo quality and intracytoplasmic sperm injection outcome. - Fertil. Steril. 94: 549-557. [DOI:10.1016/j.fertnstert.2009.02.050]
4. Barratt, C.L., Aitken, R.J., Björndahl, L., Carrell, D.T., de Boer, P., Kvist, U., Lewis, S.E., Perreault, S.D., Perry, M.J., Ramos, L., Robaire, B., Ward, S. and Zini, A. 2010. Sperm DNA: organization, protection and vulnerability: from basic science to clinical applications - A position report. - Hum. Reprod. 25: 824-838. [DOI:10.1093/humrep/dep465]
5. Benchaib, M., Lornage, J., Mazoyer, C., Lejeune, H., Salle, B. and François Guerin, J. 2007. Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. - Fertil. Steril. 87: 93-100. [DOI:10.1016/j.fertnstert.2006.05.057]
6. Brinkworth, M.H. 2000. Paternal transmission of genetic damage: findings in animals and humans. - Int. J. Androl. 23: 123-135. [DOI:10.1046/j.1365-2605.2000.00222.x]
7. Bungum, M., Humaidan, P., Axmon, A., Spano, M., Bungum, L., Erenpreiss, J. and Giwercman, A. 2007. Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. - Hum. Reprod. 22: 174-179. [DOI:10.1093/humrep/del326]
8. Campbell, A.J. and Irvine, D.S. 2000. Male infertility and intracytoplasmic sperm injection (ICSI). - Br. Med. Bull. 56: 616-629. [DOI:10.1258/0007142001903427]
9. Collins, J.A., Barnhart, K.T. and Schlegel, P.N. 2008. Do sperm DNA integrity tests predict pregnancy with in vitro fertilization? - Fertil. Steril. 89: 823-831. [DOI:10.1016/j.fertnstert.2007.04.055]
10. Duran, E.H., Morshedi, M., Taylor, S. and Oehninger, S. 2002. Sperm DNA quality predicts intrauterine insemination outcome: a prospective cohort study. - Hum. Reprod. 17: 3122-2128. [DOI:10.1093/humrep/17.12.3122]
11. Ebner, T., Yaman, C., Moser, M., Sommergruber, M., Pölz, W. and Tews, G. 2001. Embryo fragmentation in vitro and its impact on treatment and pregnancy outcome. - Fertil. Steril. 76: 281-285. [DOI:10.1016/S0015-0282(01)01904-5]
12. Hammadeh, M.E., al-Hasani, S., Stieber, M., Rosenbaum, P., Küpker, D., Diedrich, K. and Schmidt, W. 1996. The effect of chromatin condensation (aniline blue staining) and morphology (strict criteria) of human spermatozoa on fertilization, cleavage and pregnancy rates in an intracytoplasmic sperm injection programme. - Hum. Reprod. 11: 2468-2471. [DOI:10.1093/oxfordjournals.humrep.a019139]
13. Larson, K.L., DeJonge, C.J., Barnes, A.M., Jost, L.K. and Evenson, D.P. 2000. Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques. - Hum. Reprod. 15: 1717-1722. [DOI:10.1093/humrep/15.8.1717]
14. Larson-Cook, K.L., Brannian, J.D., Hansen, K.A., Kasperson, K.M., Aamold, E.T. and Evenson, D.P. 2003. Relationship between the outcomes of assisted reproductive techniques and sperm DNA fragmentation as measured by the sperm chromatin structure assay. - Fertil. Steril. 80: 895-902. [DOI:10.1016/S0015-0282(03)01116-6]
15. Morris, I.D., Ilott, S., Dixon, L. and Brison, D.R. 2002 The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development. - Hum. Reprod. 17: 990-998. [DOI:10.1093/humrep/17.4.990]
16. Pacey, A.A. 2010. Quality assurance and quality control in the laboratory andrology. - Asian J. Androl. 12: 21-25. [DOI:10.1038/aja.2009.16]
17. Perreault, S.D. 2003. Distinguishing between fertilization failure and early pregnancy loss when identifying male-mediated adverse pregnancy outcomes. - Adv. Exp. Med. Biol. 518:189-198. [DOI:10.1007/978-1-4419-9190-4_16]
18. Practice Committee of American Society for Reproductive Medicine. 2008. The clinical utility of sperm DNA integrity testing. - Fertil. Steril. 90: 178-180. [DOI:10.1016/j.fertnstert.2008.08.054]
19. Ramos, L., Kleingeld, P., Meuleman, E., van Kooy, R., Kremer, J., Braat, D. and Wetzels, A. 2002. Assessment of DNA fragmentation of spermatozoa that were surgically retrieved from men with obstructive azoospermia. - Fertil. Steril. 77: 233-237. [DOI:10.1016/S0015-0282(01)02962-4]
20. Robinson, L., Gallos, I.D., Conner, S.J., Rajkhowa, M., Miller, D., Lewis, S., Kirkman-Brown, J. and Coomarasamy, A. 2012. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta‑analysis. - Hum. Reprod. 27: 2908-2917. [DOI:10.1093/humrep/des261]
21. Sadeghi, M.R., Lakpour, N., Heidari-Vala, H., Hodjat, M., Amirjannati, N., Hossaini Jadda, H., Binaafar, S. and Akhondi, M.M. 2011. Relationship between sperm chromatin status and ICSI outcome in men with obstructiveazoospermia and unexplained infertile normozoospermia. - Rom. J. Morphol. Embryol. 52: 645-651.
22. Savitz, D.A. 2003. Paternal exposure to known mutagens and health of the offspring: ionizing radiation and tobacco smoke. - Adv. Exp. Med. Biol. 518: 49-57. [DOI:10.1007/978-1-4419-9190-4_5]
23. Scott, L., Alvero, R., Leondires, M. and Miller, B. 2000. The morphology of human pronuclear embryos is positively related to blastocyst development and implantation. - Hum. Reprod. 15: 2394-2403. [DOI:10.1093/humrep/15.11.2394]
24. Sharma, R.K., Said, T. and Agarwal, A. 2004. Sperm DNA damage and its clinical relevance in assessing reproductive outcome. - Asian. J. Androl. 6: 139-148.
25. Spanò, M., Bonde, J.P., Hjøllund, H.I., Kolstad, H.A., Cordelli, E. and Leter, G. 2000. Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team. - Fertil. Steril. 73: 43-50. [DOI:10.1016/S0015-0282(99)00462-8]
26. Tomlinson, M., Lewis, S. and Morroll, D. 2013. Sperm quality and its relationship to natural and assisted conception: British Fertility Society guidelines for practice. - Hum. Fertil. 16: 175-193. [DOI:10.3109/14647273.2013.807522]
27. Weng, S.L., Chiu, C.M., Lin, F.M., Huang, W.C., Liang, C., Yang, T., Yang, T.L., Liu, C.Y., Wu, W.Y., Chang, Y.A., Chang, T.H. and Huang, H.D. 2014. Bacterial communities in semen from men of infertile couples: metagenomics sequencing reveals relationships of seminal microbiota to semen quality. - PLOS ONE 9: e110152. [DOI:10.1371/journal.pone.0110152]
28. World Health Organization. 2010. WHO laboratory manual for the examination and processing of human semen. 5th ed. - World Health organization, Geneva. 271 pp.
29. Zini, A., Boman, J.M., Belzile, E. and Ciampi, A. 2008. Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta‑analysis. - Hum. Reprod. 23: 2663-2668. [DOI:10.1093/humrep/den321]
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