Enzymatic diversity in the haloactinomycetes isolated from rhizosphere of the plants in saline soils at the periphery of Dasht-e-Kavir desert

Salehghamari, Ensieh

doi:10.52547/nbr.9.3.193

Volume 9, Issue 3 (12-2022) nbr 2022, 9(3): 193-199 | Back to browse issues page

‎ 10.52547/nbr.9.3.193

‎ 20.1001.1.24236330.1401.9.3.4.4

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Salehghamari E. Enzymatic diversity in the haloactinomycetes isolated from rhizosphere of the plants in saline soils at the periphery of Dasht-e-Kavir desert. nbr 2022; 9 (3) :193-199
URL: http://nbr.khu.ac.ir/article-1-3535-en.html

Enzymatic diversity in the haloactinomycetes isolated from rhizosphere of the plants in saline soils at the periphery of Dasht-e-Kavir desert

Ensieh Salehghamari ^*

Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Karaj, Iran , esaleh@khu.ac.ir

Abstract: (2476 Views)

Saline rhizospheric areas are untouched environments for isolating actinomycetes with the potential of valuable salt tolerant enzyme production. In this study, we isolated and evaluated a total number of of 26 actinomycete strains from plant rhizosphere of the periphery of Dasht-e-Kavir desert. Isolated actinomycetes produced different enzymes. Among them 50, 46, 39, 27, 10 and 7 % of the isolates produced amylase, lipase, protease, gelatinase, lecithinase and urease, respectively. The most frequently produced enzymes among the isolates were amylase, lipase and protease. Combined hydrolytic activity was also detected in some actinomycete strains. Among the isolates, strains Q1, Q4 and Q11 with the most diverse enzymes production, were identified and their 16s rRNA analysis showed that they are mostly similar to the Streptomyces scopiformis, Streptomyces argenteolus and Streptomyces manipurensis, respectively. Finally, due to the enzymatic diversity obtained and the valubility of the halophilic bacterial enzymes in industry, it seems that actinomycetes isolated from this saline habitat are potentially suitable for biotechnological applications.

Keywords: amylase, halophiles, hydrolase, screening, Streptomyces

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