Volume 7, Issue 2 (7-2020)                   nbr 2020, 7(2): 145-152 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jangi M, Mohammadgholi A, Divsalr A. The investigation of the effects of synthesized Zinc oxide nanoparticles on the DNA using green chemistry . nbr. 2020; 7 (2) :145-152
URL: http://nbr.khu.ac.ir/article-1-3266-en.html
Departement of Biology, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Abstract:   (351 Views)
In this study, the extract of coffee powder for green synthesis of zinc oxide nanoparticles has been used because it is compatibility with the environment and it does not produce any toxic substances in the reaction. Then, the interaction of zinc oxide nanoparticles with calf thymus DNA with various spectroscopic methods such as UV-Visible, fluorescence and circular dichroism (CD) techniques was investigated. UV-Visible data showed that zinc oxide nanoparticles induced denaturation in DNA in a dose-dependent manner at both the room and physiologic temperatures. Extrinsic fluorescence emission of ethidium bromide (EB) results also showed that the increase of zinc oxide nanoparticles concentrations, decreases the emission intensity of EB. This may be the result of the intercalation of nanoparticles. Also, CD data showed that the synthesized zinc oxide caused structural changes in DNA. Finally, according to our results, it can be concluded that zinc oxide nanoparticles can bind with DNA and induce some structural changes in DNA structure.
 
 
Full-Text [PDF 482 kb]   (285 Downloads)    
Type of Study: Original Article | Subject: Genetics
Received: 2019/05/6 | Revised: 2020/07/1 | Accepted: 2019/09/16 | Published: 2020/06/30 | ePublished: 2020/06/30

Add your comments about this article : Your username or Email:
CAPTCHA

Creative Commons Licence
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.



© 2020 All Rights Reserved | Nova Biologica Reperta

Designed & Developed by : Yektaweb